Patents by Inventor Stephen R. Fahnestock
Stephen R. Fahnestock has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 9278138Abstract: Short single chain peptides having affinity for a target surface often lack the binding durability required for certain commercial applications. One way to improve durability is to promote multivalent binding by linking together binding sequences using peptide linkers. However, the resulting single chain binding peptides often suffer from linker entropy. It has been discovered that the use of rigid peptide linkers when linking together multiple binding sequences enhances the binding affinity of the resulting single chain peptide.Type: GrantFiled: February 27, 2014Date of Patent: March 8, 2016Assignee: E. I. DU PONT DE NEMOURS AND COMPANYInventors: Qiong Cheng, Stephen R. Fahnestock, Hongxian He, Kristy N. Kostichka, Hong Wang
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Patent number: 9115182Abstract: Compositions and methods are provided using fusion peptides comprising at least one multi-functional solubility tag having an effective number of cross-linkable cysteines residues. The multi-functional peptidic solubility tags facilitate efficient fusion peptide production, easier downstream processing of the fusion peptide, and provide functional surface properties when coupled to a target material while the cross-linkable cysteines provide enhanced durability when binding the fusion peptide to a target material.Type: GrantFiled: February 20, 2013Date of Patent: August 25, 2015Assignee: E I DU PONT DE NEMOURS AND COMPANYInventors: Stephen R Fahnestock, Kari A Fosser, Tanja Maria Gruber, Pierre E Rouviere, Linda Jane Solomon
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Patent number: 9062312Abstract: Compositions and methods using fusion peptides comprising multi-functional solubility tags are provided. The multi-functional peptidic solubility tags facilitates more efficient fusion peptide production, easier downstream processing of the fusion peptide, and may be used to provide functional surface properties when coupled to a target material.Type: GrantFiled: June 20, 2012Date of Patent: June 23, 2015Assignee: Danisco US Inc.Inventors: Pierre E Rouviere, Tanja Maria Gruber, Kari A Fosser, Stephen R Fahnestock
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Publication number: 20140241999Abstract: Short single chain peptides having affinity for a target surface often lack the binding durability required for certain commercial applications. One way to improve durability is to promote multivalent binding by linking together binding sequences using peptide linkers. However, the resulting single chain binding peptides often suffer from linker entropy. It has been discovered that the use of rigid peptide linkers when linking together multiple binding sequences enhances the binding affinity of the resulting single chain peptide.Type: ApplicationFiled: February 27, 2014Publication date: August 28, 2014Applicant: E. I. DU PONT DE NEMOURS AND COMPANYInventors: Qiong Cheng, Stephen R. Fahnestock, Hongxian He, Kristy N. Kostichka, Hong Wang
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Patent number: 8815550Abstract: Disclosed herein are compositions and methods to target enzymatic peracid production to a target surface. The peracid benefit agent produced by the targeted perhydrolytic enzyme can be use for a variety of applications such as bleaching, whitening, disinfecting, destaining, deodorizing, and combinations thereof. Specifically, a fusion protein comprising a perhydrolytic enzyme and at least one peptidic component having affinity for a target surface (excluding body surfaces and oral care surfaces) is used in combination with a suitable substrate and a source of peroxygen to enzymatically produce a peracid on or near the surface of the target material. In a preferred aspect, the target surface is a cellulosic material.Type: GrantFiled: November 13, 2013Date of Patent: August 26, 2014Assignee: E I du Pont de Nemours and CompanyInventors: Robert DiCosimo, Scott D Cunningham, Stephen R Fahnestock, Tanja Maria Gruber, Mark S Payne, Pierre E Rouviere, Linda Jane Solomon, Hong Wang
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Publication number: 20140221608Abstract: An acid-cleavable peptide linker comprising aspartic acid and proline residues is disclosed. The acid-cleavable peptide linker provides an altered sensitivity to acid-hydrolytic release of peptides of interest from fusion peptides of the formula PEP1-L-PEP2. The inventive linker, L, is described in various embodiments, each of which provides substantially more rapid acid-release of peptides of interest than does a single aspartic acid-proline pair. In an additional aspect, a method of increasing the stability of an acid cleavable linkage to acid hydrolysis is also provided.Type: ApplicationFiled: April 15, 2014Publication date: August 7, 2014Applicant: E I DU PONT DE NEMOURS AND COMPANYInventors: LAURA A BEDZYK, Stephen R. Fahnestock, Tanja Maria Gruber, Daniel P. O'Keefe, Pierre E Rouviere
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Patent number: 8748399Abstract: An acid-cleavable peptide linker comprising aspartic acid and proline residues is disclosed. The acid-cleavable peptide linker provides an altered sensitivity to acid-hydrolytic release of peptides of interest from fusion peptides of the formula PEP1-L-PEP2. The inventive linker, L, is described in various embodiments, each of which provides substantially more rapid acid-release of peptides of interest than does a single aspartic acid-proline pair. In an additional aspect, a method of increasing the stability of an acid cleavable linkage to acid hydrolysis is also provided.Type: GrantFiled: November 12, 2013Date of Patent: June 10, 2014Assignee: E I du Pont de Nemours and CompanyInventors: Laura A Bedzyk, Stephen R Fahnestock, Tanja Maria Gruber, Daniel P O'Keefe, Pierre E Rouviere
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Patent number: 8697654Abstract: Short single chain peptides having affinity for a target surface often lack the binding durability required for certain commercial applications. One way to improve durability is to promote multivalent binding by linking together binding sequences using peptide linkers. However, the resulting single chain binding peptides often suffer from linker entropy. It has been discovered that the use of rigid peptide linkers when linking together multiple binding sequences enhances the binding affinity of the resulting single chain peptide.Type: GrantFiled: December 8, 2009Date of Patent: April 15, 2014Assignee: E I du Pont de Nemours and CompanyInventors: Qiong Cheng, Stephen R. Fahnestock, Hongxian He, Kristy N. Kostichka, Hong Wang
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Publication number: 20140080998Abstract: An acid-cleavable peptide linker comprising aspartic acid and proline residues is disclosed. The acid-cleavable peptide linker provides an altered sensitivity to acid-hydrolytic release of peptides of interest from fusion peptides of the formula PEP1-L-PEP2. The inventive linker, L, is described in various embodiments, each of which provides substantially more rapid acid-release of peptides of interest than does a single aspartic acid-proline pair. In an additional aspect, a method of increasing the stability of an acid cleavable linkage to acid hydrolysis is also provided.Type: ApplicationFiled: November 12, 2013Publication date: March 20, 2014Applicant: E I DU PONT DE NEMOURS AND COMPANYInventors: LAURA A BEDZYK, Stephen R. Fahnestock, Tanja Maria Gruber, Daniel P. O'Keefe, Pierre E Rouviere
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Publication number: 20140073029Abstract: Disclosed herein are compositions and methods to target enzymatic peracid production to a target surface. The peracid benefit agent produced by the targeted perhydrolytic enzyme can be use for a variety of applications such as bleaching, whitening, disinfecting, destaining, deodorizing, and combinations thereof. Specifically, a fusion protein comprising a perhydrolytic enzyme and at least one peptidic component having affinity for a target surface (excluding body surfaces and oral care surfaces) is used in combination with a suitable substrate and a source of peroxygen to enzymatically produce a peracid on or near the surface of the target material. In a preferred aspect, the target surface is a cellulosic material.Type: ApplicationFiled: November 13, 2013Publication date: March 13, 2014Applicant: E I DU PONT DE NEMOURS AND COMPANYInventors: ROBERT DICOSIMO, Scott D. Cunningham, Stephen R. Fahnestock, Tanja Maria Gruber, Mark S. Payne, Pierre E. Rouviere, Linda Jane Solomon, Hong Wang
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Patent number: 8652455Abstract: Disclosed herein are compositions and methods to target enzymatic peracid production to a target surface. The peracid benefit agent produced by the targeted perhydrolytic enzyme can be use for a variety of applications such as bleaching, whitening, disinfecting, destaining, deodorizing, and combinations thereof. Specifically, a fusion protein comprising a perhydrolytic enzyme and at least one peptidic component having affinity for a target surface (excluding body surfaces and oral care surfaces) is used in combination with a suitable substrate and a source of peroxygen to enzymatically produce a peracid on or near the surface of the target material. In a preferred aspect, the target surface is a cellulosic material.Type: GrantFiled: December 19, 2011Date of Patent: February 18, 2014Assignee: E I du Pont de Nemours and CompanyInventors: Robert DiCosimo, Scott D. Cunningham, Stephen R. Fahnestock, Tanja Maria Gruber, Mark S. Payne, Pierre E. Rouviere, Linda Jane Solomon, Hong Wang
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Patent number: 8617843Abstract: The invention relates to the recombinant expression of a peptide of interest in the form of a fusion protein comprising a solubility tag. The fusion protein comprises at least two portions separated by a cleavable peptide sequence wherein one portion is devoid of cysteine residues and the second portion comprises an effective number of cross-linkable cysteine residues. After cell lysis and isolation of the fusion protein, the fusion protein is subsequently cleaved into a mixture of first and second portions. Oxidative cross-linking is used to selectively precipitate one of the two portions to facilitate simple and effective separation of the peptide of interest.Type: GrantFiled: April 6, 2011Date of Patent: December 31, 2013Assignee: E I du Pont de Nemours and CompanyInventors: Albert W. Alsop, Qiong Cheng, Linda Jane Solomon, Stephen R. Fahnestock, Tanja Maria Gruber, Pierre E. Rouviere
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Patent number: 8609621Abstract: An acid-cleavable peptide linker comprising aspartic acid and proline residues is disclosed. The acid-cleavable peptide linker provides an altered sensitivity to acid-hydrolytic release of peptides of interest from fusion peptides of the formula PEP1-L-PEP2. The inventive linker, L, is described in various embodiments, each of which provides substantially more rapid acid-release of peptides of interest than does a single aspartic acid-proline pair. In an additional aspect, a method of increasing the stability of an acid cleavable linkage to acid hydrolysis is also provided.Type: GrantFiled: September 7, 2011Date of Patent: December 17, 2013Assignee: E I du Pont de Nemours and CompanyInventors: Laura A. Bedzyk, Stephen R. Fahnestock, Tanja Maria Gruber, Daniel P. Okeefe, Pierre E. Rouviere
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Patent number: 8481678Abstract: Peptide-based reagents comprising at least one tooth surface-binding peptide for delivery of at least one polymer-coated white colorant to the surface of teeth are provided. The peptide-based reagent may comprise at least one tooth-binding peptide coupled to, either directly or through one or more spacers, a polymer-binding peptide capable of binding to the polymeric coating on a polymer-coated white colorant. Methods of delivering a polymer-coated white colorant to a tooth surface are also provided as well as oral care compositions comprising the present peptide-based reagents. The peptide-based reagents may be used in conjunction with at least one polymer-coated white colorant to improve the cosmetic appearance of teeth.Type: GrantFiled: February 2, 2010Date of Patent: July 9, 2013Assignee: E I du Pont de Nemours and CompanyInventors: Douglas Robert Anton, Scott D. Cunningham, Stephen R. Fahnestock, Kari A. Fosser, Hong Wang
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Publication number: 20130164353Abstract: Compositions and methods using fusion peptides comprising multi-functional solubility tags are provided. The multi-functional peptidic solubility tags facilitates more efficient fusion peptide production, easier downstream processing of the fusion peptide, and may be used to provide functional surface properties when coupled to a target material.Type: ApplicationFiled: June 20, 2012Publication date: June 27, 2013Applicant: E I DU PONT DE NEMOURS AND COMPANYInventors: PIERRE E. ROUVIERE, Tanja Maria Gruber, Kari A. Fosser, Stephen R. Fahnestock
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Publication number: 20130157927Abstract: Compositions and methods are provided using fusion peptides comprising at least one multi-functional solubility tag having an effective number of cross-linkable cysteines residues. The multi-functional peptidic solubility tags facilitate efficient fusion peptide production, easier downstream processing of the fusion peptide, and provide functional surface properties when coupled to a target material while the cross-linkable cysteines provide enhanced durability when binding the fusion peptide to a target material.Type: ApplicationFiled: February 20, 2013Publication date: June 20, 2013Inventors: STEPHEN R. FAHNESTOCK, Kari A. Fosser, Tanja Maria Gruber, Pierre E. Rouviere, Linda Jane Solomon
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Patent number: 8354381Abstract: Oral care compositions, oral care systems, oral surface-binding peptides, and a method for applying particles to an oral surface are provided. The oral care system comprises at least one peptidic component comprising a first binding element having affinity for an oral surface and a second binding element having affinity for a ligand property of a particle.Type: GrantFiled: March 26, 2010Date of Patent: January 15, 2013Assignee: E I du Pont de Nemours and CompanyInventors: Stephen R. Fahnestock, Kari A. Fosser, Hong Wang, Pierre E. Rouviere, Tanja Maria Gruber, Douglas Robert Anton
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Publication number: 20130011356Abstract: Hair-binding peptides were isolated for their use in a variety of personal care formulations and applications. The isolation of hair-binding peptides was accomplished by enrichment using mRNA-display selection technology. Hair care compositions comprising peptide-based reagents prepared comprising the hair-binding peptides are also provided.Type: ApplicationFiled: September 13, 2012Publication date: January 10, 2013Applicant: E I DU PONT DE NEMOURS AND COMPANYInventors: Stephen R. Fahnestock, Eberhard Schneider, Gregor Schurmann, Peter Wagner
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Publication number: 20120321581Abstract: Disclosed herein are compositions and methods to target enzymatic peracid production to a target surface. The peracid benefit agent produced by the targeted perhydrolytic enzyme can be use for a variety of applications such as bleaching, whitening, disinfecting, destaining, deodorizing, and combinations thereof. Specifically, a fusion protein comprising a perhydrolytic enzyme and at least one peptidic component having affinity for a target surface (excluding body surfaces and oral care surfaces) is used in combination with a suitable substrate and a source of peroxygen to enzymatically produce a peracid on or near the surface of the target material. In a preferred aspect, the target surface is a cellulosic material.Type: ApplicationFiled: December 19, 2011Publication date: December 20, 2012Applicant: E.I. DU PONT DE NEMOURS AND COMPANYInventors: Robert DiCosimo, Scott D. Cunningham, Stephen R. Fahnestock, Tanja Maria Gruber, Mark S. Payne, Pierre E. Rouviere, Linda Jane Solomon, Hong Wang
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Publication number: 20120317733Abstract: Disclosed herein are compositions and methods to treat hair with a peracid-based benefit agent. The peracid benefit agent can be used for hair bleaching, hair weakening, hair removal, hair waiving, hair straightening or any combination thereof. The peracid may be enzymatically generated from a carboxylic acid ester substrate using an enzyme having perhydrolytic activity (perhydrolase) in the presence of a source of peroxygen. A fusion protein comprising the perhydrolase coupled to a hair-binding domain, either directly or through an optional linker, may be used to target the perhydrolytic activity to the hair surface.Type: ApplicationFiled: December 19, 2011Publication date: December 20, 2012Applicant: E.I. DU PONT DE NEMOURS AND COMPANYInventors: Dexter A. Chisholm, Xiumin Cui, Scott D. Cunningham, Robert DiCosimo, Stephen R. Fahnestock, Tanja Maria Gruber, Yongqing Huang, Xueping Jiang, Anju Parthasarathy, Mark S. Payne, Pierre E. Rouviere, Hong Wang