Abstract: Systems and methods for determining cell type are provided. Information is obtained including, for each spot in a set of capture spots, a position in a tissue image and analyte abundances in the tissue. A current iteration of cell types, set to a maximum number, is determined using the analyte abundances, where each cell type has an abundance value for each analyte. When the current iteration exceeds a minimum number of cell types, a distance metric between each cell type in the current iteration is determined based on cell type analyte abundance values and the current iteration is reformed by merging a first and second cell type having a smallest distance metric. The procedure is repeated until the current iteration matches the minimum number. For each current iteration, output is determined including, for each cell type, for each spot, a proportion of cells in the spot having the cell type.

Abstract: Provided herein are methods of characterizing tumors or a region of interest in a biological sample.

Abstract: Provided herein are methods of characterizing tumors or a region of interest in a biological sample.

Abstract: Systems and methods for identifying regions of aneuploidy in a tissue include obtaining nucleic acid sequence reads, each including a spatial barcode, associating the read with a feature in a two-dimensional array of features on a substrate contacting the tissue, and a unique molecular identifier (UMI). The reads serve to determine a count data structure comprising, for each of a plurality of genomic regions, a respective UMI count for each feature in the two-dimensional array of features on the substrate. For each feature in the array of features, a respective bin count is made for each respective bin in a plurality of bins corresponding to the respective feature, where the plurality of bins span a genome. Copy number state respective features in the array are determined using feature bin counts. The copy number state of each feature in the array of features serves to identify regions of tissue aneuploidy.

Abstract: Provided herein are methods of characterizing tumors or a region of interest in a biological sample.

Abstract: Provided herein are methods of determining a location of one or more analytes of interest in a cancer sample. Also provided herein are methods of detecting expression of one or more analytes in a cancer (e.g., breast cancer) sample. Further provided are methods of diagnosing and treating breast cancer.

Abstract: Provided herein are methods of detecting biomarkers and/or candidate biomarkers for heart disorders and uses of the same.

Abstract: A roller skate having enhanced steerability and stability is disclosed. The skate includes a platform for supporting a skater's foot and front and rear wheel trucks secured to the underside of the platform. A pair of front wheels is rotatably mounted in transverse axial alignment on the front wheel truck and a pair of rear wheels is rotatably mounted in transverse axial alignment on said rear wheel truck. The pairs of front and rear wheels are also in parallel axial alignment with each other and mounted on their respective wheel trucks for resiliently controlled, tilting movement about downwardly inclined longitudinal axes. In addition, a fifth wheel is rotatably mounted on the front wheel truck between the pairs of front and rear wheels and in parallel axial alignment with the wheel pairs.

Abstract: A mop handle connector which is removably attached to the end of a mop handle and is typically fitted with a clamp to facilitate removable attachment of a dust mop frame to the mop handle. In a preferred embodiment the mop handle connector is characterized by a connector plate, one end of which is threaded into the end of the mop handle and a conventional clamping mechanism is pivotally attached to the opposite end of the connector plate. A lock ring may be slidably disposed on the connector plate and can be selectively brought into engagement with the clamping mechanism to maintain the attached dust mop frame in a rigid position with respect to the mop handle, or disengaged from the clamping mechanism to allow the attached dust mop frame to pivot with respect to the mop handle.

Abstract: Methods are provided for generating a square wave of variable duty cycle using a microprocessor and an interval timer. The duty cycle can be changed without changing the period. The duty cycle is changed on a rising or a falling edge of the signal as desired.

Abstract: A programmable digital circuit is provided for bus control of a fast microprocessor such as a V33 microprocessor. The digital circuit includes a bus controller, a wait state controller and a clock generator generating a processor clock and a system clock with but a small skew between them.

Abstract: Methods are provided for generating a square wave of variable duty cycle using a microprocessor and an interval timer. The duty cycle can be changed without changing the period. The duty cycle is changed on a rising or a falling edge of the signal as desired.

Abstract: An injection-molded receiver for securing a mop handle, broom handle or alternative cleaning or utility handle to a mop, broom or other cleaning or utility implement or tool having a threaded mounting stud. The injection-molded receiver includes a receiver cap having a cap opening for receiving and mounting one end of the handle and a cap base integrally formed in the receiver cap and fitted with an internally-threaded insert having flange cleats which is injection-molded into the cap base for threadably receiving the threaded mounting stud attached to the cleaning or utility implement, for example, a screw-type mop head. Injection-molding of the insert in the cap base of the receiver facilitates stability, longevity and ease of coupling a screw-type mop or similar utility implement or tool to a handle.

Abstract: An NMR spectroscopy method monitors a component of a sample, the component having two resonances in coupled together different spectral regions. The first resonance lies in a region including a resonance of a second component of a sample while the second resonance lies in a spectral region including a resonance of a third component of the sample. Two spin-echo pulse sequences each consisting of pulses of a predetermined frequency are applied, the spin-echo resonance spectra together being used to obtain the second resonance of the first component with the resonance of the third component suppressed.

Abstract: The present invention entails a method and apparatus for cleaning and maintaining large areas of low to medium pile carpet, such as is found in hotels, airports, etc. The invention employs a mobile carpet cleaning machine which is propelled over the carpet to be cleaned. The principal features of the carpet cleaning machine are an elongated cylindrical brush, a vacuum system with a floating vacuum head, and a blower. In operation the cylindrical brush is set into a rotating motion and engages the underlying carpet. A cleaning solution is sprayed onto the rotating brush which in turn transfers the solution onto the underlying carpet. The vacuum head then passes over the carpet directly behind the rotating brush. The blower pulls air through the vacuum head and the vacuum head effectively picks up a combined mixture of air and solution. This mixture of air and solution is pulled upwardly from the vacuum head into the top of a recovery tank.

Abstract: The present invention relates to a cleaning solution dispenser attachment for a rotary floor cleaning machine that is adapted to dispense cleaning solution on demand as the floor cleaning machine is moved over an area. Cleaning solution dispenser attachment is designed to be detachably secured to various size motor housings of a rotary floor cleaning machine, and can be rotatively adjusted thereon such that a dispenser arm or jet tube that extends outwardly therefrom can be positioned at various locations around the motor housing.