Patents by Inventor Stephen Toddo

Stephen Toddo has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Selective optimization of a ribosome binding site for protein production
    Patent number: 10696963
    Abstract: A method for optimising a nucleotide sequence for protein expression in a host cell, comprising the steps of: (a) constructing an expression library comprising a large number of variants of the sequence to be expressed operatively cloned in an expression vector, wherein (i) the sequence of the 6 nucleotides immediately upstream (5?-direction) of the first codon of the coding sequence to be expressed is completely or partially randomized; (ii) the sequence of the second and third codons of the coding sequence to be expressed is randomized, wherein the randomization of the second and third codons is limited to changes not altering the amino-acids encoded by said codons; (b) screening the library with regard to efficiency of protein expression in the host cell; and (c) selecting a sequence resulting in a desired level of efficiency of protein expression.
    Type: Grant
    Filed: December 15, 2015
    Date of Patent: June 30, 2020
    Assignee: CloneOpt AB
    Inventors: Daniel Daley, Kiavash Mirzadeh, Stephen Toddo, Suchithra Guntur
  • SELECTIVE OPTIMIZATION OF A RIBOSOME BINDING SITE FOR PROTEIN PRODUCTION
    Publication number: 20180273934
    Abstract: A method for optimising a nucleotide sequence for protein expression in a host cell, comprising the steps of: (a) constructing an expression library comprising a large number of variants of the sequence to be expressed operatively cloned in an expression vector, wherein (i) the sequence of the 6 nucleotides immediately upstream (5?-direction) of the first codon of the coding sequence to be expressed is completely or partially randomized; (ii) the sequence of the second and third codons of the coding sequence to be expressed is randomized, wherein the randomization of the second and third codons is limited to changes not altering the amino-acids encoded by said codons; (b) screening the library with regard to efficiency of protein expression in the host cell; and (c) selecting a sequence resulting in a desired level of efficiency of protein expression.
    Type: Application
    Filed: December 15, 2015
    Publication date: September 27, 2018
    Inventors: Daniel Daley, Kiavash Mirzadeh, Stephen Toddo, Suchithra Guntur