Abstract: A high sensitivity array-based detection system includes a plurality of probes immobilized onto a plurality of locations provided on a surface of a patterned array, such as a microarray. At least one target selectively binds to at least one of the plurality of probes when disposed proximate thereto under appropriate conditions, such as temperature and pH. The target includes at least one bound particle. The bound particle includes a plurality of label molecules. Alternatively, the target can be immobilized to the surface of the array, such as from a sample suspected of including the target. An excitation light source provides excitation light incident to one or more locations on the patterned array. A photodetector detects emanated signals from the patterned array. The photodetector can be a commercially available digital camera.