Abstract: Embodiments herein exploit the optical sectioning capability of reflectance confocal microscopy to non-invasively survey the dermal-epidermal junction (DEJ), noting the irregularities associated with malignancy. Methods are provided to aid a clinician in diagnosing melanoma through pattern recognition to extract pertinent diagnostic information from large 3D confocal images. Identifying the combination of pagetoid melanocytes and DEJ breakdown increases the accuracy of detection. A method may be used to process a 3D confocal volume of images taken by a clinician of a suspicious lesion and deduce the depth location z of the first reflective surface (FRS) at each x-y position. This FRS is where the most superficial melanin resides. In this manner, the stratum corneum and epidermis are digitally stripped and no longer distract the clinician from the more diagnostically relevant pigmented cell network. The FRS is putatively either the DEJ for benign nevi or the depth of a pagetoid melanocyte at x,y above the DEJ.

Abstract: Embodiments herein relate to the field of medical monitoring, and, more specifically, to a fiberoptic probe for monitoring tissue oxygenation and a method for using such a probe. A non-invasive method of measuring tissue oxygenation includes, in some embodiments, illuminating a tissue surface with a first fiberoptic fiber, receiving light from the tissue surface with a second fiberoptic fiber, measuring the absorption spectra of oxy- and deoxy-hemoglobin in the light, and calculating a tissue oxygenation value based on the absorption spectra.

Abstract: Embodiments herein exploit the optical sectioning capability of reflectance confocal microscopy to non-invasively survey the dermal-epidermal junction (DEJ), noting the irregularities associated with malignancy. Methods are provided to aid a clinician in diagnosing melanoma through pattern recognition to extract pertinent diagnostic information from large 3D confocal images. Identifying the combination of pagetoid melanocytes and DEJ breakdown increases the accuracy of detection. A method may be used to process a 3D confocal volume of images taken by a clinician of a suspicious lesion and deduce the depth location z of the first reflective surface (FRS) at each x-y position. This FRS is where the most superficial melanin resides. In this manner, the stratum corneum and epidermis are digitally stripped and no longer distract the clinician from the more diagnostically relevant pigmented cell network. The FRS is putatively either the DEJ for benign nevi or the depth of a pagetoid melanocyte at x,y above the DEJ.