Abstract: A fishing line spooling assembly includes a bracket that can be positioned on a support surface and a pair of panels attached to the bracket. A first screw is extendable through each of the panels to rotatably retain a spool of fishing line between the panels. A window is slidable into each of the panels such that the window defines a front threshold of a spool space defined between the panels. The window has a line hole extending through the window thereby facilitating fishing line on the spool of fishing line to be extended through the line hole thereby facilitating the fishing line to be wound around a reel of a fishing pole. A pair of second screws is each coupled to and extends upwardly from the lower portion of the bracket thereby facilitating a respective spool of fishing line positioned on the second screws for storage.