Patents by Inventor Steven A. Soper

Steven A. Soper has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20190346422
    Abstract: The present invention is directed to a device that comprises a biomolecular processor and one or more nanotubes. Each biomolecular processor comprises a bioreactor chamber defined by a solid substrate, a plurality of spaced support structures within said bioreactor chamber and attached to the solid substrate, and one or more capture molecules immobilized to some or all of said plurality of spaced support structures, said one or more capture molecules suitable to bind to a portion of a target nucleic acid molecule in a sample. The device also comprises one or more nanotubes defined by the solid substrate and fluidically coupled to the bioreactor chamber. Each of the one or more nanotubes has a passage extending between an input end proximate to the bioreactor chamber and an output end distal to the bioreactor chamber, and comprises one or more nanopores within the passage with each nanopore having a reduced diameter relative to the passage.
    Type: Application
    Filed: July 17, 2019
    Publication date: November 14, 2019
    Inventors: Steven A. Soper, Francis Barany, Sunggook Park, Michael Murphy, Collin J. McKinney, John William Efcavitch, Mateusz Hupert
  • Patent number: 10429376
    Abstract: Microdevices are disclosed to efficiently, accurately, and rapidly isolate and enumerate rare cells, such as circulating tumor cells, from liquids such as whole blood. The system employs multiple parallel meandering channels having a width on the order of 1-2 cell diameters. The microdevices can be produced at low-cost, may readily be automated, and in many instances may be used without pre-processing of the sample. They may be used to isolate and enumerate rare cells, including for example the detection and diagnosis of cancers, cancer staging, or evaluating the effectiveness of a therapeutic intervention, or detecting pathogenic bacteria. The device may optionally be used to nondestructively capture and later to release target cells.
    Type: Grant
    Filed: May 13, 2009
    Date of Patent: October 1, 2019
    Assignee: BOARD OF SUPERVISORS OF LOUISIANA STATE UNIVERSITY AND AGRICULTURAL AND MECHANICAL COLLEGE
    Inventors: Steven A. Soper, Michael C. Murphy, June Feng, Robin L. McCarley, André A. Adams
  • Patent number: 10416150
    Abstract: Microdevices are disclosed to efficiently, accurately, and rapidly isolate and enumerate rare cells, such as circulating tumor cells, from liquids such as whole blood. The system employs multiple parallel meandering channels having a width on the order of 1-2 cell diameters. The microdevices can be produced at low-cost, may readily be automated, and in many instances may be used without pre-processing of the sample. They may be used to isolate and enumerate rare cells, including for example the detection and diagnosis of cancers, cancer staging, or evaluating the effectiveness of a therapeutic intervention, or detecting pathogenic bacteria. The device may optionally be used to nondestructively capture and later to release target cells.
    Type: Grant
    Filed: March 26, 2018
    Date of Patent: September 17, 2019
    Assignee: BOARD OF SUPERVISORS OF LOUISIANA STATE UNIVERSITY AND AGRICULTURAL AND MECHANICAL COLLEGE
    Inventors: Steven Soper, Michael Murphy, June Feng Schneider, Robin McCarley, André Adams
  • Patent number: 10393726
    Abstract: The present invention is directed to a device that comprises a biomolecular processor and one or more nanotubes. Each biomolecular processor comprises a bioreactor chamber defined by a solid substrate, a plurality of spaced support structures within said bioreactor chamber and attached to the solid substrate, and one or more capture molecules immobilized to some or all of said plurality of spaced support structures, said one or more capture molecules suitable to bind to a portion of a target nucleic acid molecule in a sample. The device also comprises one or more nanotubes defined by the solid substrate and fluidically coupled to the bioreactor chamber.
    Type: Grant
    Filed: March 23, 2016
    Date of Patent: August 27, 2019
    Assignees: The University of North Carolina at Chapel Hill, Cornell University, Board of Supervisors of Louisiana State University and Agricultural and Mechanical College
    Inventors: Steven A. Soper, Francis Barany, Sunggook Park, Michael Murphy, Collin J. McKinney, John William Efcavitch, Mateusz Hupert
  • Publication number: 20180346973
    Abstract: The present invention is directed methods for identifying, in a sample, one or more target nucleotide sequences differing from other nucleotide sequences in the sample by one or more nucleotides, one or more copy numbers, one or more transcript sequences, and/or one or more methylated residues, using ligation detection reactions, polymerase mediated extension reactions, and/or cleavage reactions. The present invention is also directed to methods for identifying, in a sample, one or more nucleotides in a target nucleotide sequence.
    Type: Application
    Filed: March 23, 2016
    Publication date: December 6, 2018
    Inventors: Francis Barany, John William Efcavitch, Steven A. Soper, Sunggook Park
  • Publication number: 20180252698
    Abstract: Microdevices are disclosed to efficiently, accurately, and rapidly isolate and enumerate rare cells, such as circulating tumor cells, from liquids such as whole blood, The system employs multiple parallel meandering channels having a width on the order of 1-2 cell diameters. The microdevices can be produced at low-cost, may readily be automated, and in many instances may be used without pre-processing of the sample. They may he used to isolate and enumerate rare cells, including for example the detection and diagnosis of cancers, cancer staging, or evaluating the effectiveness of a therapeutic intervention, or detecting pathogenic bacteria, The device may optionally he used to nondestructively capture and later to release target cells.
    Type: Application
    Filed: March 26, 2018
    Publication date: September 6, 2018
    Inventors: Steven Soper, Michael Murphy, June Feng Schneider, Robin McCarley, André Adams
  • Publication number: 20180187257
    Abstract: The present invention relates to a device comprising a biomolecular processor. Each biomolecular processor has one or more bioreactor chambers defined by a solid substrate; a support structure within each bioreactor; a cleaving enzyme immobilized to the support structure and operatively positioned within the bioreactor chamber to cleave monomer or multimer units of a biopolymer molecule operatively engaged by the cleaving enzyme; and one or more time-of-flight channels formed in the solid substrate and fluidically coupled to said one or more bioreactor chambers. Each of the time-of-flight channels have two or more sensors including at least (i) a first sensor contacting the time-of-flight channel proximate to the input end of the channel and (ii) a second sensor contacting the time-of-flight channel proximate to the output end of channel. The present invention further relates to methods of sequencing and identifying biopolymer molecules using the device.
    Type: Application
    Filed: February 19, 2018
    Publication date: July 5, 2018
    Inventors: Steven A. SOPER, Francis BARANY, George GRILLS, Robin McCARLEY, Collin J. McKINNEY, Doral MOLDOVAN, Michael C. MURPHY, Dimitris NIKITOPOULOS, Sunggook PARK, Elizabeth J. PODLAHA-MURPHY
  • Patent number: 9952212
    Abstract: This invention provides methods and compositions for capturing circulating tumor cells (CTCs) as well as various divergent CTC phenotypes using seprase-specific affinity reagents. Methods of analyzing CTCs and assessing their metastatic potential in vivo and in vitro are also disclosed.
    Type: Grant
    Filed: December 18, 2015
    Date of Patent: April 24, 2018
    Assignees: Roche Molecular Systems, Inc., The University of North Carolina Chapel Hill
    Inventors: George Martin, Steven Soper, Makgorzata Witek, Jen Jen Yeh
  • Publication number: 20180074039
    Abstract: The present invention is directed to a device that comprises a biomolecular processor and one or more nanotubes. Each biomolecular processor comprises a bioreactor chamber defined by a solid substrate, a plurality of spaced support structures within said bioreactor chamber and attached to the solid substrate, and one or more capture molecules immobilized to some or all of said plurality of spaced support structures, said one or more capture molecules suitable to bind to a portion of a target nucleic acid molecule in a sample. The device also comprises one or more nanotubes defined by the solid substrate and fluidically coupled to the bioreactor chamber.
    Type: Application
    Filed: March 23, 2016
    Publication date: March 15, 2018
    Inventors: Steven A. Soper, Francis Barany, Sunggook Park, Michael Murphy, Collin J. Mckinney, John William Efcavitch, Mateusz Hupert
  • Patent number: 9909173
    Abstract: The present invention relates to a device comprising a biomolecular processor. Each biomolecular processor has one or more bioreactor chambers defined by a solid substrate; a support structure within each bioreactor; a cleaving enzyme immobilized to the support structure and operatively positioned within the bioreactor chamber to cleave monomer or multimer units of a biopolymer molecule operatively engaged by the cleaving enzyme; and one or more time-of-flight channels formed in the solid substrate and fluidically coupled to said one or more bioreactor chambers. Each of the time-of-flight channels have two or more sensors including at least (i) a first sensor contacting the time-of-flight channel proximate to the input end of the channel and (ii) a second sensor contacting the time-of-flight channel proximate to the output end of channel. The present invention further relates to methods of sequencing and identifying biopolymer molecules using the device.
    Type: Grant
    Filed: February 10, 2014
    Date of Patent: March 6, 2018
    Assignees: Cornell University, University of North Carolina at Chapel Hill, Northeastern University, Board of Supervisors of Louisiana State University and Agriculture and Mechanical College
    Inventors: Steven A. Soper, Francis Barany, George Grills, Robin McCarley, Collin J. McKinney, Dorel Moldovan, Michael C. Murphy, Dimitris Nikitopoulos, Sunggook Park, Elizabeth J. Podlaha-Murphy
  • Patent number: 9718676
    Abstract: A method is disclosed for fabricating free-standing polymeric nanopillars or nanotubes with remarkably high aspect ratios. The nanopillars and nanotubes may be used, for example, in integrated microfluidic systems for rapid, automated, high-capacity analysis or separation of complex protein mixtures or their enzyme digest products. One embodiment, preferably fabricated entirely from polymer substrates, comprises a cell lysis unit; a solid-phase extraction unit with free-standing, polymeric nanostructures; a multi-dimensional electrophoretic separation unit with high peak capacity; a solid-phase nanoreactor for the proteolytic digestion of isolated proteins; and a chromatographic unit for the separation of peptide fragments from the digestion of proteins.
    Type: Grant
    Filed: September 12, 2007
    Date of Patent: August 1, 2017
    Assignee: Board of Supervisors of Louisiana State University And Agricultural and Mechanical College
    Inventors: Steven A. Soper, Robin L. McCarley, Guofang Chen, Hamed Shadpour
  • Patent number: 9670540
    Abstract: The present invention is directed to methods for capturing, amplifying and identifying one or more of a plurality of target nucleotide sequences in a sample. The present invention is further directed to a device comprising a solid support having a plurality of wells or pillars and a plurality of oligonucleotides attached to the wells or pillars. Other aspects of the invention are directed to methods of making such devices.
    Type: Grant
    Filed: July 23, 2012
    Date of Patent: June 6, 2017
    Assignees: Cornell University, Board of Supervisors of Louisiana State University and Agricultural and Mechanical College
    Inventors: Francis A. Barany, Steven A. Soper, George Grills, Yu-wei Cheng, Jianmin Huang, Hong Wang, Malgorzata A. Witek, Daniel Sang-won Park, Michael C. Murphy, Robin Lindsey McCarley, Mateusz L. Hupert
  • Publication number: 20160359179
    Abstract: A fluid array, comprises: (a) a fluid input header, (b) a fluid output header, and (c) a plurality of (preferably at least 26) parallel fluid channels, each of said fluid channels connected to both said fluid input header and said fluid output header in a Z-array configuration. Methods of using the same and fuel cells comprising such arrays are also described, along with methods of optimizing flow therein.
    Type: Application
    Filed: February 24, 2015
    Publication date: December 8, 2016
    Inventors: Joshua Jackson, Mateusz L. Hupert, Steven Soper
  • Publication number: 20160139116
    Abstract: This invention provides methods and compositions for capturing circulating tumor cells (CTCs) as well as various divergent CTC phenotypes using seprase-specific affinity reagents. Methods of analyzing CTCs and assessing their metastatic potential in vivo and in vitro are also disclosed.
    Type: Application
    Filed: December 18, 2015
    Publication date: May 19, 2016
    Inventors: George Martin, Steven Soper, Makgorzata Witek, Jen Jen Yeh
  • Patent number: 9250242
    Abstract: This invention provides methods and compositions for capturing circulating tumor cells (CTCs) as well as various divergent CTC phenotypes using seprase-specific affinity reagents. Methods of analyzing CTCs and assessing their metastatic potential in vivo and in vitro are also disclosed.
    Type: Grant
    Filed: November 8, 2013
    Date of Patent: February 2, 2016
    Assignees: ROCHE MOLECULAR SYSTEMS, INC., THE UNIVERSITY OF NORTH CAROLINA
    Inventors: George Martin, Steven Soper, Makgorzata Witek, Jen Jen Yeh
  • Publication number: 20150361489
    Abstract: The present invention relates to a device comprising a biomolecular processor. Each biomolecular processor has one or more bioreactor chambers defined by a solid substrate; a support structure within each bioreactor; a cleaving enzyme immobilized to the support structure and operatively positioned within the bioreactor chamber to cleave monomer or multimer units of a biopolymer molecule operatively engaged by the cleaving enzyme; and one or more time-of-flight channels formed in the solid substrate and fluidically coupled to said one or more bioreactor chambers. Each of the time-of-flight channels have two or more sensors including at least (i) a first sensor contacting the time-of-flight channel proximate to the input end of the channel and (ii) a second sensor contacting the time-of-flight channel proximate to the output end of channel. The present invention further relates to methods of sequencing and identifying biopolymer molecules using the device.
    Type: Application
    Filed: February 10, 2014
    Publication date: December 17, 2015
    Inventors: Steven A. SOPER, Francis BARANY, George GRILLS, Robin McCARLEY, Collin J. McKINNEY, Dorel MOLDOVAN, Michael C. MURPHY, Dimitris NIKITOPOULOS, Sunggook PARK, Elizabeth J. PODLAHA-MURPHY
  • Publication number: 20150099642
    Abstract: The present invention is directed to methods for capturing, amplifying and identifying one or more of a plurality of target nucleotide sequences in a sample. The present invention is further directed to a device comprising a solid support having a plurality of wells or pillars and a plurality of oligonucleotides attached to the wells or pillars. Other aspects of the invention are directed to methods of making such devices.
    Type: Application
    Filed: July 23, 2012
    Publication date: April 9, 2015
    Applicants: Board of Supervisors of Louisiana State University and Agricultural and Mechanical College, CORNELL UNIVERSITY
    Inventors: Francis A. Barany, Steven A. Soper, George Grills, Yu-wei Cheng, Jianmin Huang, Malgorzata A. Witek, Daniel San-won Park, Michael C. Murphy, Robin Lindsey McCarley, Mateusz L. Hupert
  • Patent number: 8758974
    Abstract: A method is described for the direct photochemical modification and micro-patterning of polymer surfaces, without the need to use a photoresist. For example, micropatterns of various functional chemical groups, biomolecules, and metal films have been deposited on poly(carbonate) and poly(methyl methacrylate) surfaces. These patterns may be used, for example, in integrated electronics, capture elements, or sensing elements in micro-fluidic channels.
    Type: Grant
    Filed: December 12, 2003
    Date of Patent: June 24, 2014
    Assignee: Board of Supervisors of Louisiana State University And Agricultural and Mechanical College
    Inventors: Steven A. Soper, Robin L. McCarley, Bikas Vaidya
  • Publication number: 20140134646
    Abstract: This invention provides methods and compositions for capturing circulating tumor cells (CTCs) as well as various divergent CTC phenotypes using seprase-specific affinity reagents. Methods of analyzing CTCs and assessing their metastatic potential in vivo and in vitro are also disclosed.
    Type: Application
    Filed: November 8, 2013
    Publication date: May 15, 2014
    Applicants: ROCHE MOLECULAR SYSTEMS, INC., THE UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL, BIOFLUIDICA, INC
    Inventors: George Martin, Steven Soper, Makgorzata Witek, Jen Jen Yeh
  • Patent number: 8524891
    Abstract: Asymmetrically substituted metal-phthalocyanine compounds are disclosed. These compounds and other phthalocyanine-derivatives are used in bioimaging, bioanalysis, FRET and quenching techniques, photodynamic therapy, DNA analysis for cells, proteins, tissues and other biological entities, and other applications. Near-infrared fluorescence minimizes matrix effects typically seen in other methods of analyzing biochemical entities in cells, proteins, tissues and other biological entities.
    Type: Grant
    Filed: July 14, 2006
    Date of Patent: September 3, 2013
    Assignee: Board of Supervisors of Louisiana State University and Agricultural and Mechanical College
    Inventors: Robert P. Hammer, Steven A. Soper, Serhii Pakhomov, Timothy J. Jensen, Michael W. Allen, Irina V. Nesterova, Maria da Graça Henriques Vicente