Abstract: The present invention provides a method for the assessment of cholesterol esterification through physiologically relevant pathways and incorporates the function of individual subject's endogenous HDL particles. This ex vivo approach avoids the use of an artificial substrate and provides for determination of LCAT activity that includes both the contribution of a subject's endogenous HDL function and endogenous LCAT protein, and is therefore a significant improvement to the biologic relevance.

Abstract: The present invention relates to an improved method for the isolation of high density lipoprotein (HDL) or HDL-like particles. The method of the present invention provides for the isolation of a more highly purified HDL that requires substantially less time than standard methods. The present invention relates to an improved method for the isolation of high density lipoprotein or HDL-like particles comprising the steps of precipitation of apoprotein B-containing proteins and a single ultracentrifugation to recover high density lipoprotein or HDL-like particles.

Abstract: An engine cooling system in which a coolant flows through a coolant circuit includes: a pump for circulating coolant under pressure through the coolant circuit; a radiator provided in the coolant circuit, wherein the radiator cools coolant passing through the coolant circuit; a by-pass conduit, wherein the by-pass conduit allows coolant to by-pass the radiator, a flow control valve, which regulates the flow rate of coolant flowing through the radiator and the by-pass conduit; and a controller, wherein the controller controls the flow control valve in response to input signals from at least one pressure sensor and at least one temperature sensor in the coolant circuit. The flow control valve includes a first controllable valve located upstream of the radiator and downstream of the by-pass conduit, and a second controllable valve located in the by-pass conduit.

Abstract: An engine cooling system in which a coolant flows through a coolant circuit includes: a pump for circulating coolant under pressure through the coolant circuit; a radiator provided in the coolant circuit, wherein the radiator cools coolant passing through the coolant circuit; a by-pass conduit, wherein the by-pass conduit allows coolant to by-pass the radiator, a flow control valve, which regulates the flow rate of coolant flowing through the radiator and the by-pass conduit; and a controller, wherein the controller controls the flow control valve in response to input signals from at least one pressure sensor and at least one temperature sensor in the coolant circuit. The flow control valve includes a first controllable valve located upstream of the radiator and downstream of the by-pass conduit, and a second controllable valve located in the by-pass conduit.

Abstract: A system, method and computer program product are provided for creation of a network training environment that simulates a large network as a training target and using simulation and virtual network technologies together with actual network resources to teach computer network exploitation and computer network attack techniques in training exercises for persons responsible for safeguarding networks and for probing and attacking others' networks. The system, method, and computer program product further support integration of real hosts for more realistic exercises.