Abstract: The present invention provides purified disease detection and treatment molecule polynucleotides (mddt). Also encompassed are the polypeptides (MDDT) encoded by mddt. The invention also provides for the use of mddt, or complements, oligonucleotides, or fragments thereof in diagnostic assays. The invention further provides for vectors and host cells containing mddt for the expression of MDDT. The invention additionally provides for the use of isolated and purified MDDT to induce anitbodies and to screen libraries of compounds and the use of anti-MDDT antibodies in diagnostic assays. Also provided are microarrays containing mddt and methods of use.

Abstract: An automated apparatus for use in cleaving, deprotecting and purifying synthesized polypeptides immobilized on solid phase particles. The apparatus includes cleavage and extraction vessels where peptide cleavage from solid-phase particles, and extraction of scavenger reagents occurs, respectively, and structure for automated transfer of (i) reaction solutions into and out of the cleavage vessel, (ii) peptide solution from the cleavage vessel to the extraction vessel, and (iii) extraction solvent into the extraction vessel, in dispersed form. Excess extraction solvent is removed from a side arm in the extraction vessel.