Abstract: Described herein are digital microfluidic (DMF) devices and corresponding methods for managing reagent solution evaporation during a reaction. Reactions on the DMF devices described here are performed in an air or gas matrix. The DMF devices include a means for performing reactions at different temperatures. To address the issue of evaporation of the reaction droplet especially when the reaction is performed at higher temperatures, a means for introducing a replenishing droplet has been incorporated into the DMF device. A replenishing droplet is introduced every time when it has been determined that the reaction droplet has fallen below a threshold volume. Detection and monitoring of the reaction droplet may be through visual, optical, fluorescence, colorimetric, and/or electrical means.

Abstract: Described herein are digital microfluidic (DMF) devices and corresponding methods for managing reagent solution evaporation during a reaction. Reactions on the DMF devices described here are performed in an air or gas matrix. The DMF devices include a means for performing reactions at different temperatures. To address the issue of evaporation of the reaction droplet especially when the reaction is performed at higher temperatures, a means for introducing a replenishing droplet has been incorporated into the DMF device. A replenishing droplet is introduced every time when it has been determined that the reaction droplet has fallen below a threshold volume. Detection and monitoring of the reaction droplet may be through visual, optical, fluorescence, colorimetric, and/or electrical means.

Abstract: Described herein are digital microfluidic (DMF) devices and corresponding methods for managing reagent solution evaporation during a reaction. Reactions on the DMF devices described here are performed in an air or gas matrix. The DMF devices include a means for performing reactions at different temperatures. To address the issue of evaporation of the reaction droplet especially when the reaction is performed at higher temperatures, a means for introducing a replenishing droplet has been incorporated into the DMF device. A replenishing droplet is introduced every time when it has been determined that the reaction droplet has fallen below a threshold volume. Detection and monitoring of the reaction droplet may be through visual, optical, fluorescence, colorimetric, and/or electrical means.

Abstract: The present invention relates, in part, to engineered viruses (e.g., engineered phages), phage cocktails, and methods of producing and/or identifying viruses for targeting pathogenic bacteria.

Abstract: Described herein are digital microfluidic (DMF) devices and corresponding methods for managing reagent solution evaporation during a reaction. Reactions on the DMF devices described here are performed in an air or gas matrix. The DMF devices include a means for performing reactions at different temperatures. To address the issue of evaporation of the reaction droplet especially when the reaction is performed at higher temperatures, a means for introducing a replenishing droplet has been incorporated into the DMF device. A replenishing droplet is introduced every time when it has been determined that the reaction droplet has fallen below a threshold volume. Detection and monitoring of the reaction droplet may be through visual, optical, fluorescence, colorimetric, and/or electrical means.

Abstract: Described herein are digital microfluidic (DMF) devices and corresponding methods for managing reagent solution evaporation during a reaction. Reactions on the DMF devices described here are performed in an air or gas matrix. The DMF devices include a means for performing reactions at different temperatures. To address the issue of evaporation of the reaction droplet especially when the reaction is performed at higher temperatures, a means for introducing a replenishing droplet has been incorporated into the DMF device. A replenishing droplet is introduced every time when it has been determined that the reaction droplet has fallen below a threshold volume. Detection and monitoring of the reaction droplet may be through visual, optical, fluorescence, colorimetric, and/or electrical means.

Abstract: Described herein are digital microfluidic (DMF) devices and corresponding methods for managing reagent solution evaporation during a reaction. Reactions on the DMF devices described here are performed in an air or gas matrix. The DMF devices include a means for performing reactions at different temperatures. To address the issue of evaporation of the reaction droplet especially when the reaction is performed at higher temperatures, a means for introducing a replenishing droplet has been incorporated into the DMF device. A replenishing droplet is introduced every time when it has been determined that the reaction droplet has fallen below a threshold volume. Detection and monitoring of the reaction droplet may be through visual, optical, fluorescence, colorimetric, and/or electrical means.

Abstract: The present disclosure relates to the delivery of polynucleotides and/or oligonucleotides using silica delivery platforms, e.g., silica carriers or protocells. In particular, in the present disclosure, polynucleotides in the form of plasmids expressing siRNA may be administered as cargo in the silica delivery platform to a patient or subject to inhibit and/or treat cancer in a patient. In one aspect, the silica delivery platform that have been charged with cargo comprising plasmid DNA (in particular, CRISPR ds plasmid DNA) which expresses siRNA, shRNA, mRNA and other RNA which may be used to administer these plasmids to patients in order to effect inhibition of cancer cells (especially including apoptosis of those cancer cells) and effective and/or prophylaxis of cancer, as well as numerous pathogens, including viruses, bacteria, fungi, and/or other disease states and/or conditions. In another aspect, the silica delivery platform comprises a biological package (e.g.

Abstract: The present disclosure relates to the delivery of polynucleotides and/or oligonucleotides using silica delivery platforms, e.g., silica carriers or protocells. In particular, in the present disclosure, polynucleotides in the form of plasmids expressing siRNA may be administered as cargo in the silica delivery platform to a patient or subject to inhibit and/or treat cancer in a patient. In one aspect, the silica delivery platform that have been charged with cargo comprising plasmid DNA (in particular, CRISPR ds plasmid DNA) which expresses siRNA, shRNA, mRNA and other RNA which may be used to administer these plasmids to patients in order to effect inhibition of cancer cells (especially including apoptosis of those cancer cells) and effective and/or prophylaxis of cancer, as well as numerous pathogens, including viruses, bacteria, fungi, and/or other disease states and/or conditions. In another aspect, the silica delivery platform comprises a biological package (e.g.

Abstract: A system for enhancing a nucleic acid sample may include a one pump, a denaturing chamber; a microfluidic hydroxyapatite chromatography device configured for performing hydroxyapatite chromatography on the nucleic acid sample, a sample collector, and tubing connecting the pump with the denaturing chamber, the hydroxyapatite chromatography device and the sample collector such that the pump may be used to move the nucleic acid sample from the denaturing chamber to the hydroxyapatite chromatography device and then to the sample collector.

Abstract: A method for compensating for disruption caused by trace enablement is provided. The method includes receiving a selected target to run a program, receiving a selected program that has been identified as having a problem, and receiving a selected trace type. The method also includes enabling a trace compensator for identifying non-critical code in the selected program. The non-critical code is determined by the trace type. The method further includes running the program with a trace to identify the problem, the trace compensator causing the program to suppress the non-critical code for problem reproduction to adjust for timing discrepancies.

Abstract: An emergency notification feature for a mobile station calls for automatically and periodically activating the mobile station and polling a wireless communications network for emergency messages. Notice of an emergency is provided to a user of the mobile station through at least one of a ring tone and an automatic call-back.

Abstract: In an embodiment, a source application is divided into source task subsets, which are sent to cluster members. A cluster member receives its source task subset, compiles it into a local compiled task subset, and sends the local compiled task subset to the other cluster members. The cluster member also receives compiled task subsets from other cluster members and combines them with its local compiled task subset into a compiled application. The cluster member also creates a local symbol data subset for its source task subset and sends the local symbol data subset to the other cluster members. The cluster member also receives symbol data subsets from other cluster members and combines them with its local symbol data subset into distributed symbol data. In this way, an application may be deployed to cluster members in parallel.

Abstract: An apparatus and method is described for identification of applications that benefit from HA features and assisting a system administrator in the installation of the applications in an application server with HA features configured properly for run-time operation. In preferred embodiments, an HA policy engine allows a system administrator to set up one or more general HA policies in a policy table. In a preferred embodiment, an HA run-time engine uses the policies in the policy table to configure new applications being installed in the application server. Other embodiments include an application inspection unit that introspects the byte code of the application as well as deployment descriptors to determine which features and APIs are being used that can take advantage of HA functions.

Abstract: A method for autonomically detecting and recreating exceptions occurring in a runtime environment during software development/testing. Dynamically-generated code of an exception detecting and recreating (EDR) utility is inserted into the application programming interface (API) entry points to the server to store method call parameter states by either cloning the objects or implementing Java serialization/de-serialization. The runtime listens for exceptions to be thrown and generates a java file that allows the API to be later invoked with the stored parameters for the specific interaction that generated/caused the exception. When the application is stopped, the java files generated are packaged into an application that will run on the server and allow re-execution of the problem paths.

Abstract: Maintaining connections between application servers and databases in a multitier computer architecture, including providing, through a highly available connection proxy, connections between application servers and databases, where the highly available connection proxy implements a single call point for providing the connections; monitoring by the highly available connection proxy connected databases for database failures; and replacing by the highly available connection proxy a connection to a failing database with a connection to a replica of the failing database.

Abstract: According to the preferred embodiments, an apparatus and method is described for creating a real-time class package to class archive file mapping index that improves the performance of a class loader to load needed classes in an object oriented programming environment. In preferred embodiments, the class loader includes a mapping mechanism that creates and maintains a real-time class package to file archive mapping index. In preferred embodiments, the mapping mechanism optimizes access to the file archive mapping index. In a preferred embodiment, when the mapping mechanism finds a entry in the file archive mapping index for the package, but the class is not found, the class is searched in the class path beginning from the last file archive listed in the index to optimize the search. In other embodiments, the class loader includes a large package list that is used to further optimize access to the archive index.

Abstract: An apparatus, program product and method improve software updates processes within a logically partitioned environment by creating a new, proxy partition having substantially the same operating characteristics as an original partition, in addition to an updated software application. Network connectivity is transferred seamlessly over from the original partition to the new partition. While “hidden” from the network, the original partition continues to operate with reduced resources as the new partition takes over production operations. As such, the original partition remains in a ready state against the possibility of a problem arising with the new partition. In such an instance, network connectivity is transferred back over to the original partition, which resumes production operations as before.

Abstract: An apparatus, program product and method associate a unique key with routines in an application to enable a profiler to ascertain whether to collect profile information for a routine during its execution. A key may be generated, for example, during deployment or installation of an application in an application server or other runtime environment, and subsequently associated with the routines in the application, e.g., by storing the key in the class and/or library files for the application. By doing so, each application in a runtime environment may be uniquely identified by a profiler during execution, such that profile information will only be collected for those applications for which profiling has been enabled.

Abstract: An apparatus, program product and method generate a public key and a private key in an instant messaging server. The public key and the private key may be generated in the instant messaging server in connection with the user logging into the instant messaging server. As such, the public key and the private key may be used to encrypt and/or decrypt instant messages in connection with peer to peer instant messaging.