Patents by Inventor Steven Brentano
Steven Brentano has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11326206Abstract: The present disclosure provides compositions, methods and systems for quantifying target sequences and identifying target sequence variants.Type: GrantFiled: April 6, 2017Date of Patent: May 10, 2022Assignee: PACIFIC BIOSCIENCES OF CALIFORNIA, INC.Inventors: Yi Zhang, Steven Brentano, Eugene Tu, Kandaswamy Vijayan
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Publication number: 20190119742Abstract: The present disclosure provides compositions, methods and systems for quantifying target sequences and identifying target sequence variants.Type: ApplicationFiled: April 6, 2017Publication date: April 25, 2019Applicant: OMNIOME, INC.Inventors: Yi ZHANG, Steven BRENTANO, Eugene TU, Kandaswamy VIJAYAN
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Publication number: 20080090246Abstract: The present invention concerns oligonucleotides containing one or more modified nucleotides which increase the binding affinity of the oligonucleotides to target nucleic acids having a complementary nucleotide base sequence. These modified oligonucleotides hybridize to the target sequence at a faster rate than unmodified oligonucleotides having an identical nucleotide base sequence. Such modified oligonucleotides include oligonucleotides containing at least one 2?-O-methylribofuranosyl moiety joined to a nitrogenous base. Oligonucleotides can be modified in accordance with the present invention to preferentially bind RNA targets. The present invention also concerns methods of using these modified oligonucleotides and kits containing the same.Type: ApplicationFiled: October 26, 2007Publication date: April 17, 2008Applicant: GEN-PROBE INCORPORATEDInventors: Michael BECKER, Mehrdad MAJLESSI, Steven Brentano
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Publication number: 20080090247Abstract: The present invention concerns oligonucleotides containing one or more modified nucleotides which increase the binding affinity of the oligonucleotides to target nucleic acids having a complementary nucleotide base sequence. These modified oligonucleotides hybridize to the target sequence at a faster rate than unmodified oligonucleotides having an identical nucleotide base sequence. Such modified oligonucleotides include oligonucleotides containing at least one 2?-O-methylribofuranosyl moiety joined to a nitrogenous base. Oligonucleotides can be modified in accordance with the present invention to preferentially bind RNA targets. The present invention also concerns methods of using these modified oligonucleotides and kits containing the same.Type: ApplicationFiled: October 26, 2007Publication date: April 17, 2008Applicant: GEN-PROBE INCORPORATEDInventors: Michael BECKER, Mehrdad MAJLESSI, Steven Brentano
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Publication number: 20070299254Abstract: The present invention is directed to novel methods of synthesizing multiple copies of a target nucleic acid sequence which are autocatalytic (i.e., able to cycle automatically without the need to modify reaction conditions such as temperature, pH, or ionic strength and using the product of one cycle in the next one). In particular, the present invention discloses a method of nucleic acid amplification which is robust and efficient, while reducing the appearance of side products. The method uses only one primer, the “priming oligonucleotide,” a 3'blocked promoter oligonucleotide and optionally, a means for terminating a primer extension reaction, to amplify RNA or DNA molecules in vitro, while reducing or eliminating the formation of side products. The method of the present invention minimizes or eliminates the emergence of side products, thus providing a high level of specificity.Type: ApplicationFiled: August 26, 2005Publication date: December 27, 2007Applicant: GEN-PROBE INCORPORATEDInventors: Michael Becker, Wal-Chung Lam, Kristin Livezey, Steven Brentano, Daniel Kolk, Astrid Schroder
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Publication number: 20070202523Abstract: Novel methods of synthesizing multiple copies of a target nucleic acid sequence which are autocatalytic are disclosed (i.e., able to cycle automatically without the need to modify reaction conditions such as temperature, pH, or ionic strength and using the product of one cycle in the next one). In particular, methods of nucleic acid amplification are disclosed which are robust and efficient, while reducing the appearance of side-products. In general, the methods use priming oligonucleotides that target only one sense of a target nucleic acid, a promoter oligonucleotide modified to prevent polymerase extension from its 3?-terminus and, optionally, a means for terminating a primer extension reaction, to amplify RNA or DNA molecules in vitro, while reducing or substantially eliminating the formation of side-pro ducts. The disclosed methods minimizes or substantially eliminate the emergence of side-products, thus providing a high level of specificity.Type: ApplicationFiled: March 1, 2007Publication date: August 30, 2007Inventors: Michael BECKER, Wai-Chung Lam, Kristin Livezey, Steven Brentano, Daniel Kolk, Astrid Schroder
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Publication number: 20060263822Abstract: A method of detecting rpoB sequences of Mycobacterium tuberculosis present in a biological sample that includes steps of amplifying the M. tuberculosis rpoB sequence in vitro in a nucleic acid amplification mixture that includes specific disclosed primer sequences, and detecting the amplified sequences by using probes that provide information by their specific hybridization to portions of the amplified nucleic acid is disclosed. Compositions for amplifying and detecting in vitro the rpoB sequences of M. tuberculosis in a sample are disclosed.Type: ApplicationFiled: July 28, 2006Publication date: November 23, 2006Applicants: GEN-PROBE INCORPORATED, BIOMERIEUX S.A.Inventors: Markus Jucker, Steven Brentano, Francisco Delgado, Philippe Cleuziat
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Publication number: 20060263823Abstract: A method of detecting rpoB sequences of Mycobacterium tuberculosis present in a biological sample that includes steps of amplifying the M. tuberculosis rpoB sequence in vitro in a nucleic acid amplification mixture that includes specific disclosed primer sequences, and detecting the amplified sequences by using probes that provide information by their specific hybridization to portions of the amplified nucleic acid is disclosed. Compositions for amplifying and detecting in vitro the rpoB sequences of M. tuberculosis in a sample are disclosed.Type: ApplicationFiled: July 27, 2006Publication date: November 23, 2006Applicants: GEN-PROBE INCORPORATED, BIOMERIEUX S.A.Inventors: Markus Jucker, Steven Brentano, Francisco Delgado, Philippe Cleuziat
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Publication number: 20060194240Abstract: Compositions are described for detecting binding of an analyte to a binding partner attached to a nucleic acid hybridization switch probe that includes first and second arm sequences and a support sequence that is at least partially complementary to both arm sequences, allowing the probe under hybridization conditions to form a first conformation in the absence of the analyte and to form a second conformation in the presence of the analyte, and a label associated with the probe that produces a signal that indicates the conformation of the probe. Methods are described for detecting an analyte that forms a specific binding pair with the binding partner attached to the hybridization switch probe, thereby changing the probe from a first to a second conformation that results in a detectable signal that indicates the presence of the analyte in the sample.Type: ApplicationFiled: February 28, 2006Publication date: August 31, 2006Applicant: Gen-Probe IncorporatedInventors: Lyle Arnold, Lizhong Dai, Steven Brentano, James Russell
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Publication number: 20060046265Abstract: The present invention is directed to novel methods of synthesizing multiple copies of a target nucleic acid sequence which are autocatalytic (i.e., able to cycle automatically without the need to modify reaction conditions such as temperature, pH, or ionic strength and using the product of one cycle in the next one). In particular, the present invention discloses a method of nucleic acid amplification which is robust and efficient, while reducing the appearance of side-products. The method uses only one primer, the “priming oligonucleotide,” a promoter oligonucleotide modified to prevent polymerase extension from its 3?-terminus and, optionally, a means for terminating a primer extension reaction, to amplify RNA or DNA molecules in vitro, while reducing or substantially eliminating the formation of side-products. The method of the present invention minimizes or substantially eliminates the emergence of side-products, thus providing a high level of specificity.Type: ApplicationFiled: August 26, 2005Publication date: March 2, 2006Applicant: Gen-Probe IncorporatedInventors: Michael Becker, Wai-Chung Lam, Kristin Livezey, Steven Brentano, Daniel Kolk, Astrid Schroder
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Publication number: 20060014142Abstract: Nucleic acid oligomeric sequences and in vitro nucleic acid amplification and detection methods for detecting the presence of HAV RNA sequences in samples are disclosed. Kits comprising nucleic acid oligomers for amplifying and detecting HAV nucleic acid sequences are disclosed.Type: ApplicationFiled: July 13, 2005Publication date: January 19, 2006Applicant: Gen-Probe IncorporatedInventors: James Carlson, Steven Brentano
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Publication number: 20050260562Abstract: The present invention describes oligonucleotides targeted to HPV Type 16 and/or Type 18 nucleic acid sequences which are particularly useful to aid in detecting HPV type 16 and or 18. The oligonucleotides can aid in detecting HPV Type 16 and/or Type 18 in different ways such as by acting as hybridization assay probes, helper probes, and/or amplification primers.Type: ApplicationFiled: June 26, 2003Publication date: November 24, 2005Inventors: Patricia Gordon, Nick Carter, Steven Brentano, Philip Hammond
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Publication number: 20050227227Abstract: Sequences of nucleic acid oligonucleotides for amplifying different portions of gag and pol genes of HIV-1 and for detecting such amplified nucleic acid sequences are disclosed. Methods of amplifying and detecting HIV-1 nucleic acid in a biological sample using the amplification oligonucleotides specific for gag and pol target sequences are disclosed.Type: ApplicationFiled: June 3, 2005Publication date: October 13, 2005Inventors: Yeasing Yang, Steven Brentano, Odile Babola, Nathalie Tran, Guy Vernet
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Publication number: 20050106610Abstract: The present invention concerns oligonucleotides containing one or more modified nucleotides which increase the binding affinity of the oligonucleotides to target nucleic acids having a complementary nucleotide base sequence. These modified oligonucleotides hybridize to the target sequence at a faster rate than unmodified oligonucleotides having an identical nucleotide base sequence. Such modified oligonucleotides include oligonucleotides containing at least one 2?-O-methylribofuranosyl moiety joined to a nitrogenous base. Oligonucleotides can be modified in accordance with the present invention to preferentially bind RNA targets. The present invention also concerns methods of using these modified oligonucleotides and kits containing the same.Type: ApplicationFiled: October 26, 2004Publication date: May 19, 2005Inventors: Michael Becker, Mehrdad Majlessi, Steven Brentano
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Publication number: 20050100915Abstract: Oligonucleotides used to prime in vitro nucleic acid amplification of 16S rRNA sequences or DNA encoding 16S rRNA sequences for many species within the genus Mycobacterium are disclosed. Kits including such oligonucleotides are disclosed. Methods of detecting Mycobacterium species using the oligonucleotides in in vitro nucleic acid amplification are disclosed.Type: ApplicationFiled: September 18, 2003Publication date: May 12, 2005Inventors: Steven Brentano, Markus Jucker, Francisco Delgado, Philippe Cleuziat, Marc Rodrigue