Abstract: Embodiments of the disclosure concern methods and compositions for treating a subject with cancer, including overcoming resistance to a cancer drug using non-invasive radiotherapy frequency in combination with cancer drugs. In particular embodiments, radiofrequency therapy and cancer drugs in combination provide a synergistic benefit for cancer therapy. Use of radiofrequency therapy is an effective means to facilitate transport and perfusion of the cancer drugs in the subject.

Abstract: Various embodiments of the present disclosure pertain to methods of optimizing a treatment efficacy of a biological system by tuning a property of the biological system through the addition of an optimizing agent to the biological system. The tuning can include: (a) determining a property parameter of the biological system; (b) selecting an optimizing agent to be added to the biological system based on the determined property parameter; and (c) adding the optimizing agent to the biological system. The optimizing agent can include a kosmotropic material. The biological system can include a tissue, such as a tumor. The methods of the present disclosure can be utilized to enhance the efficacy of various treatments, such as the heat treatment of a biological system exposed to a radiofrequency field. The methods of the present disclosure can also include a step of treating the biological system.