Patents by Inventor Steven D Karlen

Steven D Karlen has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 11807875
    Abstract: The invention relates to feruloyl-CoA:monolignol transferase enzymes and nucleic acids encoding the feruloyl-CoA:monolignol transferase enzymes. The enzymes and/or the nucleic acids enable incorporation of monolignol ferulates into the lignin of plants. The monolignol ferulates include, for example, p-coumaryl ferulate, coniferyl ferulate, and/or sinapyl ferulate.
    Type: Grant
    Filed: November 30, 2020
    Date of Patent: November 7, 2023
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: John Ralph, Steven D. Karlen, Rebecca Anne Smith, Brian Fox, Emily Beebe, Craig Bingman
  • Patent number: 11807876
    Abstract: The invention is directed to p-coumaroyl-CoA:monolignol transferase enzymes, nucleic acids encoding p-coumaroyl-CoA:monolignol transferase enzymes, and inhibitory nucleic acids adapted to inhibit the expression and/or translation of p-coumaroyl-CoA:monolignol transferase RNA; expression cassettes, plant cells, and plants that have or encode such nucleic acids and enzymes; and methods of making and using such nucleic acids, enzymes, expression cassettes, cells, and plants.
    Type: Grant
    Filed: November 30, 2020
    Date of Patent: November 7, 2023
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: John Ralph, Steven D. Karlen, Rebecca Anne Smith, Brian Fox, Emily Beebe, Craig Bingman
  • Patent number: 11414372
    Abstract: Methods of detecting and, optionally, determining a level of incorporation of monolignol ester conjugates into lignin. The methods include derivatizing lignin to acylate at least a portion of free phenolic and aliphatic hydroxyls and to halogenate at least a portion of benzylic alcohols present in the lignin to yield derivatized lignin, treating the derivatized lignin with a reducing agent to cleave at least a portion of the derivatized lignin to yield lignin cleavage products, acetylating at least a portion of free hydroxyl groups in the lignin cleavage products with a labeled acetylation agent to yield labeled lignin fragments, and detecting the labeled lignin fragments.
    Type: Grant
    Filed: January 21, 2019
    Date of Patent: August 16, 2022
    Assignee: WISCONSIN ALUMNI RESEARCH FOUNDATION
    Inventors: John Ralph, Steven D Karlen, Fachuang Lu, Dharshana Padmakshan
  • Publication number: 20220002744
    Abstract: The invention is directed to BAHD acyltransferase enzymes, nucleic acids encoding BAHD acyltransferase enzymes, and inhibitory nucleic acids adapted to inhibit the expression and/or translation of BAHD acyltransferase RNA; expression cassettes, plant cells, and plants that have or encode such nucleic acids and enzymes; and methods of making and using such nucleic acids, enzymes, expression cassettes, cells, and plants.
    Type: Application
    Filed: November 8, 2019
    Publication date: January 6, 2022
    Applicants: WISCONSIN ALUMNI RESEARCH FOUNDATION, THE UNIVERSITY OF BRITISH COLUMBIA
    Inventors: Steven D. Karlen, Rebecca Anne Smith, John Ralph, Emily Beebe, Craig Bingman, Brian Fox, Shawn Mansfield, Heather Mackay, Hoon Kim, Yaseen Mottiar, Faride Unda
  • Patent number: 11186882
    Abstract: A method of liquefying biomass. The method includes the steps of contacting biomass with a solution comprising an acyl halide and an acid; and then contacting the biomass with a reductant.
    Type: Grant
    Filed: July 23, 2019
    Date of Patent: November 30, 2021
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Steven D. Karlen, John Ralph
  • Publication number: 20210207104
    Abstract: The invention relates to feruloyl-CoA:monolignol transferase enzymes and nucleic acids encoding the feruloyl-CoA:monolignol transferase enzymes. The enzymes and/or the nucleic acids enable incorporation of monolignol ferulates into the lignin of plants. The monolignol ferulates include, for example, p-coumaryl ferulate, coniferyl ferulate, and/or sinapyl ferulate.
    Type: Application
    Filed: November 30, 2020
    Publication date: July 8, 2021
    Applicant: WISCONSIN ALUMNI RESEARCH FOUNDATION
    Inventors: John Ralph, Steven D. Karlen, Rebecca Anne Smith, Brian Fox, Emily Beebe, Craig Bingman
  • Publication number: 20210095263
    Abstract: The invention is directed to p-coumaroyl-CoA:monolignol transferase enzymes, nucleic acids encoding p-coumaroyl-CoA:monolignol transferase enzymes, and inhibitory nucleic acids adapted to inhibit the expression and/or translation of p-coumaroyl-CoA:monolignol transferase RNA; expression cassettes, plant cells, and plants that have or encode such nucleic acids and enzymes; and methods of making and using such nucleic acids, enzymes, expression cassettes, cells, and plants.
    Type: Application
    Filed: November 30, 2020
    Publication date: April 1, 2021
    Applicant: WISCONSIN ALUMNI RESEARCH FOUNDATION
    Inventors: John Ralph, Steven D. Karlen, Rebecca Anne Smith, Brian Fox, Emily Beebe, Craig Bingman
  • Patent number: 10883090
    Abstract: The invention is directed to p-coumaroyl-CoA:monolignol transferase enzymes, nucleic acids encoding p-coumaroyl-CoA:monolignol transferase enzymes, and inhibitory nucleic acids adapted to inhibit the expression and/or translation of p-coumaroyl-CoA:monolignol transferase RNA; expression cassettes, plant cells, and plants that have or encode such nucleic acids and enzymes; and methods of making and using such nucleic acids, enzymes, expression cassettes, cells, and plants.
    Type: Grant
    Filed: April 18, 2018
    Date of Patent: January 5, 2021
    Assignee: WISCONSIN ALUMNI RESEARCH FOUNDATION
    Inventors: John Ralph, Steven D. Karlen, Rebecca Anne Smith, Brian Fox, Emily Beebe, Craig Bingman
  • Patent number: 10883089
    Abstract: The invention relates to feruloyl-CoA:monolignol transferase enzymes and nucleic acids encoding the feruloyl-CoA:monolignol transferase enzymes. The enzymes and/or the nucleic acids enable incorporation of monolignol ferulates into the lignin of plants. The monolignol ferulates include, for example, p-coumaryl ferulate, coniferyl ferulate, and/or sinapyl ferulate.
    Type: Grant
    Filed: April 4, 2018
    Date of Patent: January 5, 2021
    Assignee: WISCONSIN ALUMNI RESEARCH FOUNDATION
    Inventors: John Ralph, Steven D. Karlen, Rebecca Anne Smith, Brian Fox, Emily Beebe, Craig Bingman
  • Publication number: 20200056252
    Abstract: A method of liquefying biomass. The method includes the steps of contacting biomass with a solution comprising an acyl halide and an acid; and then contacting the biomass with a reductant.
    Type: Application
    Filed: July 23, 2019
    Publication date: February 20, 2020
    Inventors: Steven D. Karlen, John Ralph
  • Publication number: 20190152890
    Abstract: Described is a method of detecting and/or determining levels of incorporation of monolignol ferulate conjugate esters into lignin by derivatizing the lignin, reductively cleaving the lignin, labeling the cleavage products, and then detecting cleavage products derived from monolignol ester conjugates via differential attachment of the label.
    Type: Application
    Filed: January 21, 2019
    Publication date: May 23, 2019
    Applicant: WISCONSIN ALUMNI RESEARCH FOUNDATION
    Inventors: John Ralph, Steven D. Karlen, Fachuang Lu, Dharshana Padmakshan
  • Patent number: 10227287
    Abstract: Described is a method of detecting and/or determining levels of incorporation of monolignol ferulate conjugate esters into lignin by derivatizing the lignin, reductively cleaving the lignin, labeling the cleavage products, and then detecting cleavage products derived from monolignol ester conjugates via differential attachment of the label.
    Type: Grant
    Filed: December 31, 2015
    Date of Patent: March 12, 2019
    Assignee: WISCONSIN ALUMNI RESEARCH FOUNDATION
    Inventors: John Ralph, Steven D Karlen, Fachuang Lu, Dharshana Padmakshan
  • Publication number: 20180298353
    Abstract: The invention is directed to p-coumaroyl-CoA:monolignol transferase enzymes, nucleic acids encoding p-coumaroyl-CoA:monolignol transferase enzymes, and inhibitory nucleic acids adapted to inhibit the expression and/or translation of p-coumaroyl-CoA:monolignol transferase RNA; expression cassettes, plant cells, and plants that have or encode such nucleic acids and enzymes; and methods of making and using such nucleic acids, enzymes, expression cassettes, cells, and plants.
    Type: Application
    Filed: April 18, 2018
    Publication date: October 18, 2018
    Applicant: WISCONSIN ALUMNI RESEARCH FOUNDATION
    Inventors: John Ralph, Steven D. Karlen, Rebecca Anne Smith, Brian Fox, Emily Beebe, Craig Bingman
  • Publication number: 20180282710
    Abstract: The invention relates to feruloyl-CoA:monolignol transferase enzymes and nucleic acids encoding the feruloyl-CoA:monolignol transferase enzymes. The enzymes and/or the nucleic acids enable incorporation of monolignol ferulates into the lignin of plants. The monolignol ferulates include, for example, p-coumaryl ferulate, coniferyl ferulate, and/or sinapyl ferulate.
    Type: Application
    Filed: April 4, 2018
    Publication date: October 4, 2018
    Applicant: WISCONSIN ALUMNI RESEARCH FOUNDATON
    Inventors: John Ralph, Steven D. Karlen, Rebecca Anne Smith, Brian Fox, Emily Beebe, Craig Bingman
  • Publication number: 20160187345
    Abstract: Described is a method of detecting and/or determining levels of incorporation of monolignol ferulate conjugate esters into lignin by derivatizing the lignin, reductively cleaving the lignin, labeling the cleavage products, and then detecting cleavage products derived from monolignol ester conjugates via differential attachment of the label.
    Type: Application
    Filed: December 31, 2015
    Publication date: June 30, 2016
    Applicant: WISCONSIN ALUMNI RESEARCH FOUNDATION
    Inventors: John Ralph, Steven D Karlen, Fachuang Lu, Dharshana Padmakshan