Patents by Inventor Steven D Karlen
Steven D Karlen has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Patent number: 11807875Abstract: The invention relates to feruloyl-CoA:monolignol transferase enzymes and nucleic acids encoding the feruloyl-CoA:monolignol transferase enzymes. The enzymes and/or the nucleic acids enable incorporation of monolignol ferulates into the lignin of plants. The monolignol ferulates include, for example, p-coumaryl ferulate, coniferyl ferulate, and/or sinapyl ferulate.Type: GrantFiled: November 30, 2020Date of Patent: November 7, 2023Assignee: Wisconsin Alumni Research FoundationInventors: John Ralph, Steven D. Karlen, Rebecca Anne Smith, Brian Fox, Emily Beebe, Craig Bingman
-
Patent number: 11807876Abstract: The invention is directed to p-coumaroyl-CoA:monolignol transferase enzymes, nucleic acids encoding p-coumaroyl-CoA:monolignol transferase enzymes, and inhibitory nucleic acids adapted to inhibit the expression and/or translation of p-coumaroyl-CoA:monolignol transferase RNA; expression cassettes, plant cells, and plants that have or encode such nucleic acids and enzymes; and methods of making and using such nucleic acids, enzymes, expression cassettes, cells, and plants.Type: GrantFiled: November 30, 2020Date of Patent: November 7, 2023Assignee: Wisconsin Alumni Research FoundationInventors: John Ralph, Steven D. Karlen, Rebecca Anne Smith, Brian Fox, Emily Beebe, Craig Bingman
-
Patent number: 11414372Abstract: Methods of detecting and, optionally, determining a level of incorporation of monolignol ester conjugates into lignin. The methods include derivatizing lignin to acylate at least a portion of free phenolic and aliphatic hydroxyls and to halogenate at least a portion of benzylic alcohols present in the lignin to yield derivatized lignin, treating the derivatized lignin with a reducing agent to cleave at least a portion of the derivatized lignin to yield lignin cleavage products, acetylating at least a portion of free hydroxyl groups in the lignin cleavage products with a labeled acetylation agent to yield labeled lignin fragments, and detecting the labeled lignin fragments.Type: GrantFiled: January 21, 2019Date of Patent: August 16, 2022Assignee: WISCONSIN ALUMNI RESEARCH FOUNDATIONInventors: John Ralph, Steven D Karlen, Fachuang Lu, Dharshana Padmakshan
-
Publication number: 20220002744Abstract: The invention is directed to BAHD acyltransferase enzymes, nucleic acids encoding BAHD acyltransferase enzymes, and inhibitory nucleic acids adapted to inhibit the expression and/or translation of BAHD acyltransferase RNA; expression cassettes, plant cells, and plants that have or encode such nucleic acids and enzymes; and methods of making and using such nucleic acids, enzymes, expression cassettes, cells, and plants.Type: ApplicationFiled: November 8, 2019Publication date: January 6, 2022Applicants: WISCONSIN ALUMNI RESEARCH FOUNDATION, THE UNIVERSITY OF BRITISH COLUMBIAInventors: Steven D. Karlen, Rebecca Anne Smith, John Ralph, Emily Beebe, Craig Bingman, Brian Fox, Shawn Mansfield, Heather Mackay, Hoon Kim, Yaseen Mottiar, Faride Unda
-
Patent number: 11186882Abstract: A method of liquefying biomass. The method includes the steps of contacting biomass with a solution comprising an acyl halide and an acid; and then contacting the biomass with a reductant.Type: GrantFiled: July 23, 2019Date of Patent: November 30, 2021Assignee: Wisconsin Alumni Research FoundationInventors: Steven D. Karlen, John Ralph
-
Publication number: 20210207104Abstract: The invention relates to feruloyl-CoA:monolignol transferase enzymes and nucleic acids encoding the feruloyl-CoA:monolignol transferase enzymes. The enzymes and/or the nucleic acids enable incorporation of monolignol ferulates into the lignin of plants. The monolignol ferulates include, for example, p-coumaryl ferulate, coniferyl ferulate, and/or sinapyl ferulate.Type: ApplicationFiled: November 30, 2020Publication date: July 8, 2021Applicant: WISCONSIN ALUMNI RESEARCH FOUNDATIONInventors: John Ralph, Steven D. Karlen, Rebecca Anne Smith, Brian Fox, Emily Beebe, Craig Bingman
-
Publication number: 20210095263Abstract: The invention is directed to p-coumaroyl-CoA:monolignol transferase enzymes, nucleic acids encoding p-coumaroyl-CoA:monolignol transferase enzymes, and inhibitory nucleic acids adapted to inhibit the expression and/or translation of p-coumaroyl-CoA:monolignol transferase RNA; expression cassettes, plant cells, and plants that have or encode such nucleic acids and enzymes; and methods of making and using such nucleic acids, enzymes, expression cassettes, cells, and plants.Type: ApplicationFiled: November 30, 2020Publication date: April 1, 2021Applicant: WISCONSIN ALUMNI RESEARCH FOUNDATIONInventors: John Ralph, Steven D. Karlen, Rebecca Anne Smith, Brian Fox, Emily Beebe, Craig Bingman
-
Patent number: 10883090Abstract: The invention is directed to p-coumaroyl-CoA:monolignol transferase enzymes, nucleic acids encoding p-coumaroyl-CoA:monolignol transferase enzymes, and inhibitory nucleic acids adapted to inhibit the expression and/or translation of p-coumaroyl-CoA:monolignol transferase RNA; expression cassettes, plant cells, and plants that have or encode such nucleic acids and enzymes; and methods of making and using such nucleic acids, enzymes, expression cassettes, cells, and plants.Type: GrantFiled: April 18, 2018Date of Patent: January 5, 2021Assignee: WISCONSIN ALUMNI RESEARCH FOUNDATIONInventors: John Ralph, Steven D. Karlen, Rebecca Anne Smith, Brian Fox, Emily Beebe, Craig Bingman
-
Patent number: 10883089Abstract: The invention relates to feruloyl-CoA:monolignol transferase enzymes and nucleic acids encoding the feruloyl-CoA:monolignol transferase enzymes. The enzymes and/or the nucleic acids enable incorporation of monolignol ferulates into the lignin of plants. The monolignol ferulates include, for example, p-coumaryl ferulate, coniferyl ferulate, and/or sinapyl ferulate.Type: GrantFiled: April 4, 2018Date of Patent: January 5, 2021Assignee: WISCONSIN ALUMNI RESEARCH FOUNDATIONInventors: John Ralph, Steven D. Karlen, Rebecca Anne Smith, Brian Fox, Emily Beebe, Craig Bingman
-
Publication number: 20200056252Abstract: A method of liquefying biomass. The method includes the steps of contacting biomass with a solution comprising an acyl halide and an acid; and then contacting the biomass with a reductant.Type: ApplicationFiled: July 23, 2019Publication date: February 20, 2020Inventors: Steven D. Karlen, John Ralph
-
Publication number: 20190152890Abstract: Described is a method of detecting and/or determining levels of incorporation of monolignol ferulate conjugate esters into lignin by derivatizing the lignin, reductively cleaving the lignin, labeling the cleavage products, and then detecting cleavage products derived from monolignol ester conjugates via differential attachment of the label.Type: ApplicationFiled: January 21, 2019Publication date: May 23, 2019Applicant: WISCONSIN ALUMNI RESEARCH FOUNDATIONInventors: John Ralph, Steven D. Karlen, Fachuang Lu, Dharshana Padmakshan
-
Patent number: 10227287Abstract: Described is a method of detecting and/or determining levels of incorporation of monolignol ferulate conjugate esters into lignin by derivatizing the lignin, reductively cleaving the lignin, labeling the cleavage products, and then detecting cleavage products derived from monolignol ester conjugates via differential attachment of the label.Type: GrantFiled: December 31, 2015Date of Patent: March 12, 2019Assignee: WISCONSIN ALUMNI RESEARCH FOUNDATIONInventors: John Ralph, Steven D Karlen, Fachuang Lu, Dharshana Padmakshan
-
Publication number: 20180298353Abstract: The invention is directed to p-coumaroyl-CoA:monolignol transferase enzymes, nucleic acids encoding p-coumaroyl-CoA:monolignol transferase enzymes, and inhibitory nucleic acids adapted to inhibit the expression and/or translation of p-coumaroyl-CoA:monolignol transferase RNA; expression cassettes, plant cells, and plants that have or encode such nucleic acids and enzymes; and methods of making and using such nucleic acids, enzymes, expression cassettes, cells, and plants.Type: ApplicationFiled: April 18, 2018Publication date: October 18, 2018Applicant: WISCONSIN ALUMNI RESEARCH FOUNDATIONInventors: John Ralph, Steven D. Karlen, Rebecca Anne Smith, Brian Fox, Emily Beebe, Craig Bingman
-
Publication number: 20180282710Abstract: The invention relates to feruloyl-CoA:monolignol transferase enzymes and nucleic acids encoding the feruloyl-CoA:monolignol transferase enzymes. The enzymes and/or the nucleic acids enable incorporation of monolignol ferulates into the lignin of plants. The monolignol ferulates include, for example, p-coumaryl ferulate, coniferyl ferulate, and/or sinapyl ferulate.Type: ApplicationFiled: April 4, 2018Publication date: October 4, 2018Applicant: WISCONSIN ALUMNI RESEARCH FOUNDATONInventors: John Ralph, Steven D. Karlen, Rebecca Anne Smith, Brian Fox, Emily Beebe, Craig Bingman
-
Publication number: 20160187345Abstract: Described is a method of detecting and/or determining levels of incorporation of monolignol ferulate conjugate esters into lignin by derivatizing the lignin, reductively cleaving the lignin, labeling the cleavage products, and then detecting cleavage products derived from monolignol ester conjugates via differential attachment of the label.Type: ApplicationFiled: December 31, 2015Publication date: June 30, 2016Applicant: WISCONSIN ALUMNI RESEARCH FOUNDATIONInventors: John Ralph, Steven D Karlen, Fachuang Lu, Dharshana Padmakshan