Abstract: The present invention relates to agricultural practices for maximizing carbon sequestration, enhanced productivity, sustainable farming and minimizing greenhouse gas emissions. In one embodiment, there is provided a method comprising: planting a Brassica carinata variety as a second crop in rotation with a first crop or to replace fallow; implementing land management practices to reduce the use of fossil fuel inputs and to maximize the capture of atmospheric carbon by the plant material of Brassica carinata; harvesting of the Brassica carinata variety to obtain the grain; and returning about 70% to about 90% of all plant material from the Brassica carinata variety aside from the grain to the soil. As a result, the overall greenhouse gas emissions associated with agriculture are reduced. In some embodiments, the method further comprises producing grain for use in the production of a plant-based feedstock for producing low carbon intensity fuels; for adding carbon in soil; and/or acquiring a carbon credit.

Abstract: The present invention relates to agricultural practices for maximizing carbon sequestration, enhanced productivity, sustainable farming and minimizing greenhouse gas emissions. In one embodiment, there is provided a method comprising: planting a Brassica carinata variety as a second crop in rotation with a first crop or to replace fallow; implementing land management practices to reduce the use of fossil fuel inputs and to maximize the capture of atmospheric carbon by the plant material of Brassica carinata; harvesting of the Brassica carinata variety to obtain the grain; and returning about 70% to about 90% of all plant material from the Brassica carinata variety aside from the grain to the soil. As a result, the overall greenhouse gas emissions associated with agriculture are reduced. In some embodiments, the method further comprises producing grain for use in the production of a plant-based feedstock for producing low carbon intensity fuels; for adding carbon in soil; and/or acquiring a carbon credit.

Abstract: The present invention relates to transgenic plants comprising a plurality of nucleic acids heterologous to said plant, each of said nucleic acid comprising a coding sequence operably linked to one or more regulatory elements for directing expression of said coding sequence in said plant, said nucleic acid being stably integrated at or adjacent to rDNA sequences, or a seed, organ, tissue, part or cell thereof, or a descendant of said plant, seed, organ, tissue, part or cell; methods of producing the transgenic plants; and methods of producing oil using the transgenic plants.

Abstract: The present invention relates to transgenic plants comprising a plurality of nucleic acids heterologous to said plant, each of said nucleic acid comprising a coding sequence operably linked to one or more regulatory elements for directing expression of said coding sequence in said plant, said nucleic acid being stably integrated at or adjacent to rDNA sequences, or a seed, organ, tissue, part or cell thereof, or a descendant of said plant, seed, organ, tissue, part or cell; methods of producing the transgenic plants; and methods of producing oil using the transgenic plants.

Abstract: The present invention relates to transgenic plants comprising a plurality of nucleic acids heterologous to said plant, each of said nucleic acid comprising a coding sequence operably linked to one or more regulatory elements for directing expression of said coding sequence in said plant, said nucleic acid being stably integrated at or adjacent to rDNA sequences, or a seed, organ, tissue, part or cell thereof, or a descendant of said plant, seed, organ, tissue, part or cell; methods of producing the transgenic plants; and methods of producing oil using the transgenic plants.

Abstract: The present invention relates to transgenic plants comprising a plurality of nucleic acids heterologous to said plant, each of said nucleic acid comprising a coding sequence operably linked to one or more regulatory elements for directing expression of said coding sequence in said plant, said nucleic acid being stably integrated at or adjacent to rDNA sequences, or a seed, organ, tissue, part or cell thereof, or a descendant of said plant, seed, organ, tissue, part or cell; methods of producing the transgenic plants; and methods of producing oil using the transgenic plants.

Abstract: Methods for preparing cell lines that contain plant artificial chromosomes, methods for preparation of plant artificial chromosomes, methods for targeted insertion of heterologous DNA into plant artificial chromosomes, and methods for delivery of plant chromosomes to selected cells and tissues are provided. In particular, plant artificial chromosomes that are substantially composed of repeated nucleic acid units of varying amounts of heterochromatin and euchromatin are provided. Also provided are methods of using plant and animal artificial chromosomes in the production of valuable transgenic plants. Methods for identifying plant genes encoding particular traits using artificial chromosomes and for producing an acrocentric plant chromosome also are provided.

Abstract: The present invention relates to transgenic plants comprising a plurality of nucleic acids heterologous to said plant, each of said nucleic acid comprising a coding sequence operably linked to one or more regulatory elements for directing expression of said coding sequence in said plant, said nucleic acid being stably integrated at or adjacent to rDNA sequences, or a seed, organ, tissue, part or cell thereof, or a descendant of said plant, seed, organ, tissue, part or cell; methods of producing the transgenic plants; and methods of producing oil using the transgenic plants.

Abstract: The present invention relates to transgenic plant cell cultures comprising transgenic plant cells comprising a plurality of nucleic acids heterologous to said plant, each of said nucleic acids comprising a coding sequence encoding a pharmaceutical product of interest operably linked to one or more regulatory elements for directing expression of said coding sequence in said plant cell, said nucleic acids being stably integrated at or adjacent to native rDNA of said plant cell; methods of producing the transgenic plant cell culture; and methods of producing a pharmaceutical product of interest using the transgenic plant cell culture.

Abstract: Methods for preparing cell lines that contain plant artificial chromosomes, methods for preparation of plant artificial chromosomes, methods for targeted insertion of heterologous DNA into plant artificial chromosomes, and methods for delivery of plant chromosomes to selected cells and tissues are provided. In particular, plant artificial chromosomes that are substantially composed of repeated nucleic acid units of varying amounts of heterochromafin and euchromatin are provided. Also provided are methods of using plant and animal artificial chromosomes in the production of valuable transgenic plants. Methods for identifying plant genes encoding particular traits using artificial chromosomes and for producing an acrocentric plant chromosome also are provided.

Abstract: Methods for preparing cell lines that contain plant artificial chromosomes, methods for preparation of plant artificial chromosomes, methods for targeted insertion of heterologous DNA into plant artificial chromosomes, and methods for delivery of plant chromosomes to selected cells and tissues are provided. In particular, plant artificial chromosomes that are substantially composed of repeated nucleic acid units of varying amounts of heterochromatin and euchromatin are provided. Also provided are methods of using plant and animal artificial chromosomes in the production of valuable transgenic plants. Methods for identifying plant genes encoding particular traits using artificial chromosomes and for producing an acrocentric plant chromosome are also provided.

Abstract: The present invention relates to methods to control the spread of recombinant DNA molecules between sexually compatible plants of differing genetic composition. The invention describes the production of transgenic plants that comprise recombinant traits of interest or concern linked to repressible lethal genes. The lethal genes are blocked by the action of repressor molecules produced by the expression of repressor genes located at a different genetic locus. The lethal phenotype is only expressed after the segregation of the repressible lethal gene construct and the repressor gene following meiosis. The present invention may be employed for both open-pollinated and hybrid seed production systems and may be used to maintain genetic purity by blocking unintended introgression of genes from plants devoid of the specific repressor gene.

Abstract: Genetic constructs, transformation vectors and methods are taught for production of transgenic plants which can be selectively removed from a growing site by application of a chemical agent or physiological stress. The invention links a target gene for the trait of commercial interest to a conditionally lethal gene, which can be selectively expressed to cause plant death. By use of the genetic constructs, transformation vectors and methods of the present invention, invasion of environments and contamination of commercial non-engineered productions by transgenic plants can be avoided. Methods are also taught for transformation of Brassica species.

Abstract: Methods for preparing cell lines that contain plant artificial chromosomes, methods for preparation of plant artificial chromosomes, methods for targeted insertion of heterologous DNA into plant artificial chromosomes, and methods for delivery of plant chromosomes to selected cells and tissues are provided. In particular, plant artificial chromosomes that are substantially composed of repeated nucleic acid units of varying amounts of heterochromatin and euchromatin are provided. Also provided are methods of using plant and animal artificial chromosomes in the production of valuable transgenic plants. Methods for identifying plant genes encoding particular traits using artificial chromosomes and for producing an acrocentric plant chromosome are also provided.