Abstract: Provided herein are novel IGF2 peptides, fusion proteins, and nucleic acid sequences encoding novel IGF2 peptides and fusion proteins for the treatment of lysorsomal storage disorders, wherein the IGF2 peptides confer enhanced properties, such as enhanced expression, secretion and cellular uptake. The constructs provided herein are useful in treating lysosomal storage disorders by both enzyme replacement therapy and gene therapy.

Abstract: Methods, computing devices, and magnetic resonance imaging systems that improve image quality in turbo spiral echo (TSE) imaging are disclosed. With this technology, a TSE pulse sequence is generated that includes a series of radio frequency (RF) refocusing pulses to produce a corresponding series of nuclear magnetic resonance (NMR) spin echo signals. A gradient waveform including a plurality of segments is generated. The plurality of segments collectively comprise a spiral ring retraced in-out trajectory. During an interval adjacent to each of the series of RF refocusing pulses, a first gradient pulse is generated according to the gradient waveform. The first gradient pulses encode the NMR spin echo signals. An image is then constructed from digitized samples of the NMR spin echo signals obtained based at least in part on the encoding.

Abstract: Real-time, hardware-implementable Structured Similarity (SSIM)-based rate distortion optimization (RDO) techniques for video transmission are described. The disclosed techniques provide efficient application of SSIM as a distortion metric in selecting prediction modes for encoding video for transmission. A prediction mode, at a high level, specifies which previously encoded group of pixels can be utilized to encode a subsequent block of pixels in a video frame. A less compute intensive distortion metric is first used to select a subset of candidate prediction modes. Then a more compute intensive SSIM-based selection is made on the subset. By utilizing the disclosed techniques during video encoding, tradeoffs between distortion and transmission rate can be made that are more relevant to human perception.

Abstract: Real-time, hardware-implementable Structured Similarity (SSIM)-based rate distortion optimization (RDO) techniques for video transmission are described. The disclosed techniques provide efficient application of SSIM as a distortion metric in selecting prediction modes for encoding video for transmission. A prediction mode, at a high level, specifies which previously encoded group of pixels can be utilized to encode a subsequent block of pixels in a video frame. A less compute intensive distortion metric is first used to select a subset of candidate prediction modes. Then a more compute intensive SSIM-based selection is made on the subset. By utilizing the disclosed techniques during video encoding, tradeoffs between distortion and transmission rate can be made that are more relevant to human perception.

Abstract: A system and method for imaging biological samples on multiple surfaces of a support structure are disclosed. The support structure may be a flow cell through which a reagent fluid is allowed to flow and interact with the biological samples. Excitation radiation from at least one radiation source may be used to excite the biological samples on multiple surfaces. In this manner, fluorescent emission radiation may be generated from the biological samples and subsequently captured and detected by detection optics and at least one detector. The detected fluorescent emission radiation may then be used to generate image data. This imaging of multiple surfaces may be accomplished either sequentially or simultaneously. In addition, the techniques of the present invention may be used with any type of imaging system. For instance, both epifluorescent and total internal reflection methods may benefit from the techniques of the present invention.