Abstract: The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. The methods of the invention include correcting one or more phenomena that are encountered during nucleotide sequencing, such as using sequencing by synthesis methods. These phenomena include, without limitation, sequence lead, sequence lag, spectral crosstalk, and noise resulting from variations in illumination and/or filter responses.

Abstract: The present invention relates to methods and devices for amplifying nucleic acid, and, in particular, amplifying so as to generate products on a surface without the use of emulsions. In a preferred embodiment, a plurality of groups of amplified product are generated on the surface, each group positioned in different (typically predetermined) locations on said surface so as to create an array.

Abstract: A closure (1) having a shell (10) and a polymer insert (20). The polymer material of the insert (20) is selected to be sufficiently soft to provide an adequate sealing surface (21) and yet sufficiently stiff to define legs (22), which clip behind the internal curl (11) of the closure shell (10).

Abstract: A non-pyrotechnic cueing device for use in non-lethal gunfire activities. The cueing device includes a fluid container for containing a fluid, and a fluid discharge assembly in communication with the fluid container. The fluid discharge assembly is adapted to force the fluid from the fluid container through an opening of the fluid container upon activation. A trigger having an engaged position with respect to the fluid discharge assembly and a disengaged position with respect to the fluid discharge assembly is adapted to activate the fluid discharge assembly upon movement to the disengaged position. A thermally responsive non-pyrotechnic trigger actuator is coupled to the trigger and is adapted to selectively move the trigger to the disengaged position.

Abstract: Embodiments include a non-pyrotechnic detonation simulator. The simulator includes a substrate, a plurality of capacitors fixed with respect to the substrate, a voltage source, and a controller. The controller is electrically coupled to the capacitors and is adapted to selectively direct a burst voltage from the voltage source to one or more of the capacitors to cause the one or more of the plurality of capacitors to burst.

Abstract: A rod activated water valve includes a valve base that mates with a standard water faucet. One end of the valve base attaches to a cylindrical valve housing which has an inward protruding lip, at least two key holes at a bottom end and an outer groove. A rod activated valve mechanism is housed within the cylindrical valve housing. A cylindrical outer sleeve is rotatably held onto the cylindrical valve housing by a C-shaped spring member and substantially covers the cylindrical valve housing thereby preventing removal of the cylindrical outer sleeve from the cylindrical valve housing and allowing free rotation of the cylindrical outer sleeve with respect to the cylindrical valve housing. A tool is required to be inserted into the key holes for installation and removal of the rod activated water valve with tamper-resistant security feature to/from a faucet.

Abstract: An apparatus for communicating simulation data is disclosed. The apparatus includes an assembly for mounting the apparatus on an edifice. The apparatus further includes a receiver for receiving simulation data from a plurality of players within a specified area of the edifice and for receiving simulation data from a central controller. The apparatus further includes a transmitter for transmitting simulation data to the plurality of players and to the central controller, wherein simulation data is transmitted to the central controller only when a simulation event occurs. In one alternative, the apparatus further includes an infrared camera for capturing video and still images of simulation events. In another alternative, the apparatus includes an infrared radiator for producing infrared light for illuminating video and still images captured by the infrared camera.

Abstract: The invention provides methods and compositions, and systems for determining the identity of nucleic acids in nucleotide sequences, including sequences with one or more homopolymer regions. The methods of the invention include improvements so as to accurately identify sequences, including the difficult homopolymer sequences that are encountered during nucleotide sequencing, such as pyrosequencing.

Abstract: A rod activated water valve includes a valve base that mates with a standard water faucet. One end of the valve base attaches to a cylindrical valve housing which has an inward protruding lip, at least two key holes at a bottom end and an outer groove. A rod activated valve mechanism is housed within the cylindrical valve housing. A cylindrical outer sleeve is rotatably held onto the cylindrical valve housing by a C-shaped spring member and substantially covers the cylindrical valve housing thereby preventing removal of the cylindrical outer sleeve from the cylindrical valve housing and allowing free rotation of the cylindrical outer sleeve with respect to the cylindrical valve housing. A tool is required to be inserted into the key holes for installation and removal of the rod activated water valve with tamper-resistant security feature to/from a faucet.

Abstract: A rod activated water valve includes a valve base that mates with a standard water faucet. A valve housing attaches to the second end of the valve base, and has an inward protruding lip at a bottom end. The valve includes a water flow chamber with a solid cylindrical outer surface and a substantially flat foraminous surface at one end. An aperture is located central to the flat foraminous surface and a top edge of the water flow chamber has a lip for holding an upper plate o-ring. A rod having a rod head at one end passes through the aperture and is held in place by the rod head. A valve stem for selectively blocking the flow of water from the water faucet to the water flow chamber, activated by the rod head and providing a gradual stopping of the flow of water, thereby reducing water hammer.

Abstract: The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. A plurality of smaller flow cells is employed, each with a relatively small area to be imaged, in order to provide greater flexibility and efficiency.

Abstract: The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. The methods of the invention include correcting one or more phenomena that are encountered during nucleotide sequencing, such as using sequencing by synthesis methods. These phenomena include, without limitation, sequence lead, sequence lag, spectral crosstalk, and noise resulting from variations in illumination and/or filter responses.

Abstract: An imaging system for exciting and measuring fluorescence on or in samples comprising fluorescent materials (e.g. fluorescent labels, dyes or pigments). In one embodiment, a device is used to detect fluorescent labels on nucleic acid. In a preferred embodiment, the device is configured such that fluorescent labels in a plurality of different DNA templates are simultaneously detected.

Abstract: The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. The methods of the invention include correcting one or more phenomena that are encountered during nucleotide sequencing, such as using sequencing by synthesis methods. These phenomena include, without limitation, sequence lead, sequence lag, spectral crosstalk, and noise resulting from variations in illumination and/or filter responses.

Abstract: The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. The methods of the invention include correcting one or more phenomena that are encountered during nucleotide sequencing, such as using sequencing by synthesis methods. These phenomena include, without limitation, sequence lead, sequence lag, spectral crosstalk, and noise resulting from variations in illumination and/or filter responses.

Abstract: A closure (1) having a shell (10) and a polymer insert (20). The polymer material of the insert (20) is selected to be sufficiently soft to provide an adequate sealing surface (21) and yet sufficiently stiff to define legs (22), which clip behind the internal curl (11) of the closure shell (10).

Abstract: The present invention relates to methods and devices for amplifying nucleic acid, and, in particular, amplifying so as to generate products on a surface without the use of emulsions. In a preferred embodiment, a plurality of groups of amplified product are generated on the surface, each group positioned in different (typically predetermined) locations on said surface so as to create an array.

Abstract: An application for a rod activated water valve includes a valve base that mates with a standard water faucet. The top end has a ridge for capturing a washer. A valve housing attaches to the second end of the valve base, and has an inner diameter and an inward protruding lip at a bottom end. The valve includes a water flow chamber with a solid cylindrical outer surface and a substantially flat foraminous surface at one end. An aperture is located central to the flat foraminous surface and a top edge of the water flow chamber has a lip for holding an upper plate o-ring. A rod having a rod head at one end passes through the aperture and is held in place by the rod head. There is a valve stem for selectively blocking the flow of water from the water faucet to the water flow chamber, activated by the rod head and providing a gradual stopping of the flow of water, thereby reducing water hammer.

Abstract: The present invention relates to methods and devices for amplifying nucleic acid, and, in particular, amplifying so as to generate products on a surface without the use of emulsions. In a preferred embodiment, a plurality of groups of amplified product are generated on the surface, each group positioned in different (typically predetermined) locations on said surface so as to create an array.

Abstract: The present invention relates to methods and devices for amplifying nucleic acid, and, in particular, amplifying so as to generate products on a surface without the use of emulsions. In a preferred embodiment, a plurality of groups of amplified product are generated on the surface, each group positioned in different (typically predetermined) locations on said surface so as to create an array.