Patents by Inventor Steven L. Evans
Steven L. Evans has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230272410Abstract: This disclosure concerns compositions and methods for increasing the expression of a polynucleotide of interest. Some embodiments concern novel transactivation polypeptides and variants thereof that have been identified in plants, and methods of using the same. Particular embodiments concern the use of at least one DNA-binding polypeptide in a fusion protein to target at least one transactivation polypeptide or variant thereof to a specific binding site on a nucleic acid comprising the polynucleotide of interest, such that its expression may be increased.Type: ApplicationFiled: January 20, 2023Publication date: August 31, 2023Applicant: CORTEVA AGRISCIENCE LLCInventors: JOSEPH F. PETOLINO, JIANQUAN LI, STEVEN L. EVANS, RYAN C. BLUE
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Patent number: 11603535Abstract: This disclosure concerns compositions and methods for increasing the expression of a polynucleotide of interest. Some embodiments concern novel transactivation polypeptides and variants thereof that have been identified in plants, and methods of using the same. Particular embodiments concern the use of at least one DNA-binding polypeptide in a fusion protein to target at least one transactivation polypeptide or variant thereof to a specific binding site on a nucleic acid comprising the polynucleotide of interest, such that its expression may be increased.Type: GrantFiled: October 19, 2020Date of Patent: March 14, 2023Assignee: CORTEVA AGRISCIENCE LLCInventors: Joseph F. Petolino, Jianquan Li, Steven L. Evans, Ryan C. Blue
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Publication number: 20210087241Abstract: This disclosure concerns compositions and methods for increasing the expression of a polynucleotide of interest. Some embodiments concern novel transactivation polypeptides and variants thereof that have been identified in plants, and methods of using the same. Particular embodiments concern the use of at least one DNA-binding polypeptide in a fusion protein to target at least one transactivation polypeptide or variant thereof to a specific binding site on a nucleic acid comprising the polynucleotide of interest, such that its expression may be increased.Type: ApplicationFiled: October 19, 2020Publication date: March 25, 2021Applicant: Dow AgroSciences LLCInventors: Joseph F. Petolino, Jianquan Li, Steven L. Evans, Ryan C. Blue
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Patent number: 10836804Abstract: This disclosure concerns compositions and methods for increasing the expression of a polynucleotide of interest. Some embodiments concern novel transactivation polypeptides and variants thereof that have been identified in plants, and methods of using the same. Particular embodiments concern the use of at least one DNA-binding polypeptide in a fusion protein to target at least one transactivation polypeptide or variant thereof to a specific binding site on a nucleic acid comprising the polynucleotide of interest, such that its expression may be increased.Type: GrantFiled: May 2, 2019Date of Patent: November 17, 2020Assignee: Dow Agrosciences LLCInventors: Joseph Petolino, Jianquan Li, Steven L. Evans, Ryan C. Blue
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Publication number: 20190330292Abstract: This disclosure concerns compositions and methods for increasing the expression of a polynucleotide of interest. Some embodiments concern novel transactivation polypeptides and variants thereof that have been identified in plants, and methods of using the same. Particular embodiments concern the use of at least one DNA-binding polypeptide in a fusion protein to target at least one transactivation polypeptide or variant thereof to a specific binding site on a nucleic acid comprising the polynucleotide of interest, such that its expression may be increased.Type: ApplicationFiled: May 2, 2019Publication date: October 31, 2019Inventors: Joseph Petolino, Jianquan Li, Steven L. Evans, Ryan C. Blue
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Patent number: 10415046Abstract: The present invention claims methods for the stable integration of exogenous DNA into a specific locus, E32, in the maize genome through the use of zinc finger nucleases. Maize plants and plant parts that were transformed by the methods of the invention are claimed. The invention is useful for creating desirable traits such as herbicide resistance, herbicide tolerance, insect resistance, insect tolerance, disease resistance, disease tolerance, stress tolerance, and stress resistance in maize The E32 locus represents a superior site for inserting foreign genes because native agronomic phenotypes are not disturbed.Type: GrantFiled: October 31, 2016Date of Patent: September 17, 2019Assignee: Dow AgroSciences LLCInventors: W. Michael Ainley, James W. Bing, David R. Corbin, Steven L. Evans, Joseph F. Petolino, Lakshmi Sastry-Dent, Steven A. Thompson, Steven R. Webb, Mary E. Welter, Ning Zhou
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Patent number: 10351610Abstract: This disclosure concerns compositions and methods for increasing the expression of a polynucleotide of interest. Some embodiments concern novel transactivation polypeptides and variants thereof that have been identified in plants, and methods of using the same. Particular embodiments concern the use of at least one DNA-binding polypeptide in a fusion protein to target at least one transactivation polypeptide or variant thereof to a specific binding site on a nucleic acid comprising the polynucleotide of interest, such that its expression may be increased.Type: GrantFiled: February 1, 2013Date of Patent: July 16, 2019Assignee: Dow AgroSciences LLCInventors: Joseph Petolino, Jianquan Li, Steven L Evans, Ryan C Blue
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Patent number: 10077449Abstract: A universal donor polynucleotide is described that can be inserted at targeted locations in plant genomes to facilitate rapid and high throughput integration of a donor molecule within a specific genomic location.Type: GrantFiled: November 3, 2014Date of Patent: September 18, 2018Assignee: Dow AgroSciences LLCInventors: Lakshmi Sastry-Dent, Steven L. Evans, Ryan C. Blue, Zehui Cao
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Publication number: 20170364226Abstract: A software application for use on a mobile phone or other computerized hardware system for collecting geological core samples and assigning these samples to boxes is disclosed. The mobile application can include a scanner such as a bar code scanner for reading identifying data from boxes storing cores, and also provides the ability to print barcodes for identifying boxes of cores at a printer.Type: ApplicationFiled: September 5, 2017Publication date: December 21, 2017Inventor: Steven L. Evans
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Patent number: 9752154Abstract: This invention is related to methods and systems for vector assembly for transgenic plants. A uniform modular process is used to reduce cycle time and the methods and systems provided herein can increase cloning throughput using multiple-well plates, for example 96-well plates. In some embodiments, the methods and systems provided herein eliminate or reduce the need for sequencing confirmation because no PCR is involved in the vector assembly process.Type: GrantFiled: July 23, 2013Date of Patent: September 5, 2017Assignee: Dow AgroSciences LLCInventors: Sandeep Kumar, Steven L. Evans, Manju Gupta
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Publication number: 20170044558Abstract: The present invention claims methods for the stable integration of exogenous DNA into a specific locus, E32, in the maize genome through the use of zinc finger nucleases. Maize plants and plant parts that were transformed by the methods of the invention are claimed. The invention is useful for creating desirable traits such as herbicide resistance, herbicide tolerance, insect resistance, insect tolerance, disease resistance, disease tolerance, stress tolerance, and stress resistance in maize The E32 locus represents a superior site for inserting foreign genes because native agronomic phenotypes are not disturbed.Type: ApplicationFiled: October 31, 2016Publication date: February 16, 2017Inventors: W. Michael AINLEY, James W. BING, David R. CORBIN, Steven L. EVANS, Joseph F. PETOLINO, Lakshmi SASTRY-DENT, Steven A. THOMPSON, Steven R. WEBB, Mary E. WELTER, Ning ZHOU
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Publication number: 20150176015Abstract: This invention is related to methods and systems for vector assembly for transgenic plants. A uniform modular process is used to reduce cycle time and the methods and systems provided herein can increase cloning throughput using multiple-well plates, for example 96-well plates. In some embodiments, the methods and systems provided herein eliminate or reduce the need for sequencing confirmation because no PCR is involved in the vector assembly process.Type: ApplicationFiled: July 23, 2013Publication date: June 25, 2015Applicant: DOW AGROSCIENCES LLCInventors: Sandeep Kumar, Steven L. Evans, Manju Gupta
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Publication number: 20150143588Abstract: A universal donor polynucleotide is described that can be inserted at targeted locations in plant genomes to facilitate rapid and high throughput integration of a donor molecule within a specific genomic location.Type: ApplicationFiled: November 3, 2014Publication date: May 21, 2015Inventors: Lakshmi SASTRY-DENT, Steven L. EVANS, Ryan C. BLUE, Zehui CAO
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Patent number: 8771708Abstract: Cysteine-specific radiolabeled Cry1Fa protein retains insecticidal activity against insect pests and binds to insect brush border membrane vesicle receptors in a saturable manner. The biologically-active radiolabeled Cry1Fa protein is useful in competitive binding assays with other Cry toxins.Type: GrantFiled: December 16, 2011Date of Patent: July 8, 2014Assignee: Dow AgroSciences LLCInventors: Steven L. Evans, Jianquan Li, Joel J. Sheets
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Publication number: 20140173783Abstract: The present invention claims methods for the stable integration of exogenous DNA into a specific locus, E32, in the maize genome through the use of zinc finger nucleases. Maize plants and plant parts that were transformed by the methods of the invention are claimed. The invention is useful for creating desirable traits such as herbicide resistance, herbicide tolerance, insect resistance, insect tolerance, disease resistance, disease tolerance, stress tolerance, and stress resistance in maize The E32 locus represents a superior site for inserting foreign genes because native agronomic phenotypes are not disturbed.Type: ApplicationFiled: December 13, 2013Publication date: June 19, 2014Inventors: W. Michael AINLEY, James W. BING, David H. CORBIN, Steven L. EVANS, Joseph F. PETOLINO, Lakshmi SASTRY-DENT, Steven A. THOMPSON, Steven R. WEBB, Mary E. WELTER, Ning ZHOU
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Publication number: 20140089835Abstract: A software application for use on a mobile phone or other computerized hardware system for collecting geological core samples and assigning these samples to boxes is disclosed. The mobile application can include a scanner such as a bar code scanner for reading identifying data from boxes storing cores, and also provides the ability to print barcodes for identifying boxes of cores at a printer.Type: ApplicationFiled: September 20, 2013Publication date: March 27, 2014Inventor: Steven L. Evans
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Publication number: 20120156803Abstract: Cysteine-specific radiolabeled Cry1Fa protein retains insecticidal activity against insect pests and binds to insect brush border membrane vesicle receptors in a saturable manner. The biologically-active radiolabeled Cry1Fa protein is useful in competitive binding assays with other Cry toxins.Type: ApplicationFiled: December 16, 2011Publication date: June 21, 2012Applicant: DOW AGROSCIENCES LLCInventors: STEVEN L. EVANS, JIANQUAN LI, JOEL J. SHEETS
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Patent number: 7939651Abstract: This invention provides modified, insecticidal Cry35 proteins with enhanced properties as compared to wild-type Cry35 proteins. The modifications to these proteins were based in part on analysis of the atomic coordinates and three-dimensional (3D) structure of the ˜45 kDa 149B1 protein and other proteins in the Cry35 class. The subject invention also includes polynucleotides that encode these modified proteins, and transgenic plants that produce these modified proteins. This invention further provides methods of controlling plant pests, including rootworms, with these modified proteins. The modified proteins of the subject invention include chimeric toxins involving exchanged segments, domains, and motifs as discussed herein. The subject invention also provides methods of modifying Cry35 proteins.Type: GrantFiled: March 2, 2010Date of Patent: May 10, 2011Assignee: Dow AgroSciences LLCInventors: H. Ernest Schnepf, Kenneth E. Narva, Steven L. Evans
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Patent number: 7888069Abstract: The subject application provides various compositions of matter directed to West Nile virus (WNV) polypeptides and fragments thereof and polynucleotides, vectors and transformed host cells that encode, direct the expression of, or produce WNV polypeptides as set forth herein. Methods of using the polypeptides and polynucleotides for the production of immune responses in individuals or detecting the presence of WNV specific or neutralizing antibodies are also provided herein.Type: GrantFiled: December 21, 2007Date of Patent: February 15, 2011Assignees: Dow Agrosciences LLC, The United States of America as represented by the Secretary of ArgricultureInventors: Kelley A. Smith, Steven R. Webb, Steven L. Evans, Charles A. Mihaliak, Donald J. Merlo, Geoffrey J. Letchworth
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Patent number: D663749Type: GrantFiled: December 13, 2010Date of Patent: July 17, 2012Inventor: Steven L Evans