Abstract: The present invention provides methods and compositions for providing graft recipients with epidermal melanocytes. Specifically, the methods and compositions of the invention provide for populations of epidermal melanocytes that may be isolated from a patient and cultured in vitro to generate a proliferating population of epidermal melanocytes that exhibit growth, migration and melanin production. The invention is based on the discovery of a culture medium composed of natural components, for culturing epidermal melanocytes that exhibit increased proliferative capacity, migratory behaviors and melanin production. The invention provides novel in vitro methods for culturing epidermal melanocytes, including those isolated from the skin of a healthy subject, to generate a proliferating population of epidermal melanocytes. The methods and compositions of the invention may be used for transplantation to treat patients having hypopigmentation or depigmentation skin disorders.

Abstract: A system and method for exposing ocular tissue to a beam of electromagnetic radiation tuned to an NIR wavelength between about 1380 nm and about 1600 nm in the water absorption band. The laser energy is absorbed by the water, which in turn, transfers the energy to collagen molecules. The helical collagen molecules partially denature and then re-nature to form a weld across the region of the tissues to be joined. In a preferred embodiment, the electromagnetic radiation originates from a laser. The lasers may be Cr4+ doped crystals, Erbium fibers, and alloys of semiconductor laser diodes.