Patents by Inventor Steven Menchen
Steven Menchen has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Publication number: 20210277464Abstract: Provided are systems and methods for analyte detection and analysis. A system can comprise an open substrate. The open substrate may be configured to rotate or otherwise move. The open substrate can comprise an array of individually addressable locations, with analytes immobilized thereto. The substrate may be spatially indexed to identify nucleic acid molecules from one or more sources, and/or sequences thereof, with the respective one or more sources. A solution comprising a plurality of probes may be directed across the array to couple at least one of the plurality of probes with at least one of the analytes to form a bound probe. A detector can be configured to detect a signal from the bound probe via scanning of the substrate while minimizing temperature fluctuations of the substrate or optical aberrations caused by bubbles.Type: ApplicationFiled: May 5, 2021Publication date: September 9, 2021Inventors: Nathan BECKETT, Gilad ALMOGY, Nathan CASWELL, Jacob A. WOLF, Kristopher BARBEE, Denis PRISTINSKI, Mark PRATT, Gene POLOVY, Osip SCHWARTZ, Stephanie KUBECKA, Steven MENCHEN, Joseph ANTHONY, Jose Martin SOSA, Phillip You Fai LEE
-
Publication number: 20210199647Abstract: Provided are systems and methods for analyte detection and analysis. A system can comprise an open substrate. The open substrate may be configured to rotate or otherwise move. The open substrate can comprise an array of individually addressable locations, with analytes immobilized thereto. The substrate may be spatially indexed to identify nucleic acid molecules from one or more sources, and/or sequences thereof, with the respective one or more sources. A solution comprising a plurality of probes may be directed across the array to couple at least one of the plurality of probes with at least one of the analytes to form a bound probe. A detector can be configured to detect a signal from the bound probe via scanning of the substrate while minimizing temperature fluctuations of the substrate or optical aberrations caused by bubbles.Type: ApplicationFiled: February 22, 2021Publication date: July 1, 2021Inventors: Nathan BECKETT, Gilad ALMOGY, Nathan CASWELL, Jacob A. WOLF, Kristopher BARBEE, Denis PRISTINSKI, Mark PRATT, Gene POLOVY, Osip SCHWARTZ, Stephanie KUBECKA, Steven MENCHEN, Joseph ANTHONY, Jose Martin SOSA, Phillip You Fai LEE
-
Patent number: 11047004Abstract: The present disclosure provide systems, compositions, methods, reagents, kits and products for extending a nucleic acid that includes incorporating a nucleotide residue at a terminus of a nucleic acid using a polymerase enzyme and at least one nucleotide, wherein the at least one nucleotide includes a thiophosphate moiety, and wherein the at least one nucleotide is resistant to hydrolysis by phosphatase. In some embodiments, the nucleotide incorporation can be conducted in the presence of a phosphatase. In some embodiments, the nucleotide incorporation can be conducted in the presence of at least on chelation moiety that is configured to bind an orthophosphate moiety.Type: GrantFiled: May 23, 2019Date of Patent: June 29, 2021Assignee: Life Technologies CorporationInventors: Steven Menchen, Theo Nikiforov, Barnett Rosenblum
-
Patent number: 11034853Abstract: The invention is directed to methods of modifying metal oxide or hydroxylated polymer surfaces using compositions of water soluble polymers that adsorb onto such surfaces and that contain functional groups which directly modify such surfaces without further processing. In some embodiments, compositions used in such methods include water-soluble oxide-adsorbing polymers having water solubility in an indicated temperature range, an indicated concentration in the aqueous solution, and a molecular weight range, wherein each of the water-soluble oxide-adsorbing polymers comprises a linear copolymer comprising a first monomer having at least one hydrophilic moiety and a second monomer having at least one lipophilic moiety and wherein a first monomer: second monomer molecular ratio is at least 3:1.Type: GrantFiled: January 30, 2018Date of Patent: June 15, 2021Assignee: Quantapore, Inc.Inventor: Steven Menchen
-
Publication number: 20210079464Abstract: Provided are systems and methods for analyte detection and analysis. A system can comprise an open substrate. The open substrate may be configured to rotate or otherwise move. The open substrate can comprise an array of individually addressable locations, with analytes immobilized thereto. The substrate may be spatially indexed to identify nucleic acid molecules from one or more sources, and/or sequences thereof, with the respective one or more sources. A solution comprising a plurality of probes may be directed across the array to couple at least one of the plurality of probes with at least one of the analytes to form a bound probe. A detector can be configured to detect a signal from the bound probe via scanning of the substrate while minimizing temperature fluctuations of the substrate or optical aberrations caused by bubbles.Type: ApplicationFiled: November 19, 2020Publication date: March 18, 2021Inventors: Nathan BECKETT, Gilad ALMOGY, Nathan CASWELL, Jacob A. WOLF, Kristopher BARBEE, Denis PRINTINSKI, Mark PRATT, Gene POLOVY, Osip SCHWARTZ, Stephanie KUBECKA, Steven MENCHEN, Joseph ANTHONY, Jose Martin SOSA, Phillip You Fai LEE
-
Publication number: 20200326327Abstract: Provided are systems and methods for analyte detection and analysis. A system can comprise an open substrate configured to rotate. The open substrate can comprise an array of immobilized analytes. A solution comprising a plurality of probes may be directed, via centrifugal force, across the array during rotation of the substrate, to couple at least one of the plurality of probes with at least one of the analytes to form a bound probe. A detector can be configured to detect a signal from the bound probe via continuous rotational area scanning of the substrate.Type: ApplicationFiled: April 29, 2020Publication date: October 15, 2020Inventors: Kristopher BARBEE, Nathan BECKETT, Denis PRISTINSKI, Derek SCHULTE, Avishai BARTOV, Jamie SULLIVAN, Dumitru BRINZA, Abizar LAKDAWALLA, Steven MENCHEN, Gilad ALMOGY, Mark PRATT
-
Publication number: 20200318170Abstract: The disclosure provides methods for sequencing nucleic acids using, including with nucleotide analogs and subsequently appended labels.Type: ApplicationFiled: March 18, 2020Publication date: October 8, 2020Inventors: Linda LEE, Steven MENCHEN, Theo NIKIFOROV, Gilad ALMOGY, Florian OBERSTRASS
-
Publication number: 20200199652Abstract: Disclosed herein are mesylate containing compositions, methods of use of such compositions and kits of components encompassing such compositions for improving the amplification of nucleic acids, especially in the presence of enzymatic inhibitors. These find particular use in forensic and environmental analyses. The instant disclosure provides new compositions, methods and kits of components for overcoming PCR inhibition. In some embodiments disclosed herein is a PCR master mix, the PCR master mix encompassing a mesylate.Type: ApplicationFiled: June 20, 2018Publication date: June 25, 2020Inventors: Chang ZHONG, Julio MULERO, Steven MENCHEN
-
Publication number: 20200148910Abstract: The invention is directed to methods of modifying metal oxide or hydroxylated polymer surfaces using compositions of water soluble polymers that adsorb onto such surfaces and that contain functional groups which directly modify such surfaces without further processing. In some embodiments, compositions used in such methods include water-soluble oxide-adsorbing polymers having water solubility in an indicated temperature range, an indicated concentration in the aqueous solution, and a molecular weight range, wherein each of the water-soluble oxide-adsorbing polymers comprises a linear copolymer comprising a first monomer having at least one hydrophilic moiety and a second monomer having at least one lipophilic moiety and wherein a first monomer: second monomer molecular ratio is at least 3:1.Type: ApplicationFiled: January 30, 2018Publication date: May 14, 2020Applicant: Quantapore, Inc.Inventor: Steven MENCHEN
-
Patent number: 10487357Abstract: In some embodiments, the disclosure relates generally to methods, as well as related, systems, compositions, kits and apparatuses, for nucleic acid analysis that involve the use of modified nucleotides, including terminator nucleotides and/or tagged nucleotides, in a template-dependent nucleotide incorporation reaction. In some embodiments, the nucleic acid analysis can be conducted at a single reaction site, or at a plurality of reaction sites in an array of reaction sites. Optionally, the array contains a plurality of reaction sites having about 1-100 million, or about 100-250 million, or about 200-500 million, or about 500-900 million, or more reaction sites. Optionally, each reaction site is in contact with, operatively coupled, or capacitively coupled to one or more sensors that are ion-sensitive FETs (isFETs) or chemically-sensitive FETs (chemFETs) sensors. Optionally, the reaction sites are in fluid communication with each other.Type: GrantFiled: March 18, 2016Date of Patent: November 26, 2019Assignees: Life Technologies Corporation, Life Technologies GmbHInventors: Wolfgang Hinz, Steven Menchen, Ronald Graham, Peter Vander Horn, Earl Hubbell, Christian Woehler, Roman Rozhkov, Barnett Rosenblum
-
Publication number: 20190345549Abstract: The present disclosure provide systems, compositions, methods, reagents, kits and products for extending a nucleic acid that includes incorporating a nucleotide residue at a terminus of a nucleic acid using a polymerase enzyme and at least one nucleotide, wherein the at least one nucleotide includes a thiophosphate moiety, and wherein the at least one nucleotide is resistant to hydrolysis by phosphatase. In some embodiments, the nucleotide incorporation can be conducted in the presence of a phosphatase. In some embodiments, the nucleotide incorporation can be conducted in the presence of at least on chelation moiety that is configured to bind an orthophosphate moiety.Type: ApplicationFiled: May 23, 2019Publication date: November 14, 2019Inventors: Steven MENCHEN, Theo NIKIFOROV, Barnett ROSENBLUM
-
Patent number: 10344328Abstract: Provided are methods for biological sample processing and analysis. A method can comprise providing a substrate configured to rotate. The substrate can comprise an array having immobilized thereto a biological analyte. A solution comprising a plurality of probes may be directed, via centrifugal force, across the substrate during rotation of the substrate, to couple at least one of the plurality of probes with the biological analyte. A detector can be configured to detect a signal from the at least one probe coupled to the biological analyte, thereby analyzing the biological analyte.Type: GrantFiled: May 8, 2018Date of Patent: July 9, 2019Assignee: ULTIMA GENOMICS, INC.Inventors: Kristopher Barbee, Nathan Beckett, Denis Pristinski, Derek Schulte, Avishai Bartov, Jamie Sullivan, Dumitru Brinza, Abizar Lakdawalla, Steven Menchen, Gilad Almogy, Mark Pratt
-
Patent number: 10344325Abstract: The present disclosure provide systems, compositions, methods, reagents, kits and products for extending a nucleic acid that includes incorporating a nucleotide residue at a terminus of a nucleic acid using a polymerase enzyme and at least one nucleotide, wherein the at least one nucleotide includes a thiophosphate moiety, and wherein the at least one nucleotide is resistant to hydrolysis by phosphatase. In some embodiments, the nucleotide incorporation can be conducted in the presence of a phosphatase. In some embodiments, the nucleotide incorporation can be conducted in the presence of at least on chelation moiety that is configured to bind an orthophosphate moiety.Type: GrantFiled: November 10, 2015Date of Patent: July 9, 2019Assignee: Life Technologies CorporationInventors: Steven Menchen, Theo Nikiforov, Barnett Rosenblum
-
Publication number: 20190153520Abstract: Provided are systems and methods for analyte detection and analysis. A system can comprise an open substrate configured to rotate. The open substrate can comprise an array of immobilized analytes. A solution comprising a plurality of probes may be directed, via centrifugal force, across the array during rotation of the substrate, to couple at least one of the plurality of probes with at least one of the analytes to form a bound probe. A detector can be configured to detect a signal from the bound probe via continuous rotational area scanning of the substrate.Type: ApplicationFiled: May 8, 2018Publication date: May 23, 2019Inventors: Kristopher BARBEE, Nathan BECKETT, Denis PRISTINSKI, Derek SCHULTE, Avishai BARTOV, Jamie SULLIVAN, Dumitru BRINZA, Abizar LAKDAWALLA, Steven MENCHEN, Gilad ALMOGY, Mark PRATT
-
Publication number: 20190153531Abstract: Provided are methods for biological sample processing and analysis. A method can comprise providing a substrate configured to rotate. The substrate can comprise an array having immobilized thereto a biological analyte. A solution comprising a plurality of probes may be directed, via centrifugal force, across the substrate during rotation of the substrate, to couple at least one of the plurality of probes with the biological analyte. A detector can be configured to detect a signal from the at least one probe coupled to the biological analyte, thereby analyzing the biological analyte.Type: ApplicationFiled: May 8, 2018Publication date: May 23, 2019Inventors: Kristopher BARBEE, Nathan BECKETT, Denis PRISTINSKI, Derek SCHULTE, Avishai BARTOV, Jamie SULLIVAN, Dumitru BRINZA, Abizar LAKDAWALLA, Steven MENCHEN, Gilad ALMOGY, Mark PRATT
-
Patent number: 10273528Abstract: Provided are systems and methods for analyte detection and analysis. A system can comprise an open substrate configured to rotate. The open substrate can comprise an array of immobilized analytes. A solution comprising a plurality of probes may be directed, via centrifugal force, across the array during rotation of the substrate, to couple at least one of the plurality of probes with at least one of the analytes to form a bound probe. A detector can be configured to detect a signal from the bound probe via continuous rotational area scanning of the substrate.Type: GrantFiled: May 8, 2018Date of Patent: April 30, 2019Assignee: ULTIMA GENOMICS, INC.Inventors: Kristopher Barbee, Nathan Beckett, Denis Pristinski, Derek Schulte, Avishai Bartov, Jamie Sullivan, Dumitru Brinza, Abizar Lakdawalla, Steven Menchen, Gilad Almogy, Mark Pratt
-
Patent number: 10267790Abstract: Provided are systems for biological sample processing and analysis. A system can comprise a substrate configured to rotate. The substrate can comprise an array configured to immobilize a biological analyte. A fluid flow unit comprising a fluid channel can be configured to dispense a solution comprising a plurality of probes. The solution may be directed, via centrifugal force, across the substrate during rotation of the substrate, to couple at least one of the plurality of probes with the biological analyte. A detector in optical communication with the array can be configured to detect one or more signals from the at least one probe coupled to the biological analyte.Type: GrantFiled: May 8, 2018Date of Patent: April 23, 2019Assignee: ULTIMA GENOMICS, INC.Inventors: Kristopher Barbee, Nathan Beckett, Denis Pristinski, Derek Schulte, Avishai Bartov, Jamie Sullivan, Dumitru Brinza, Abizar Lakdawalla, Steven Menchen, Gilad Almogy, Mark Pratt
-
Publication number: 20180119217Abstract: In some embodiments, the disclosure relates generally to methods, as well as related, systems, compositions, kits and apparatuses, for nucleic acid analysis that involve the use of modified nucleotides, including terminator nucleotides and/or tagged nucleotides, in a template-dependent nucleotide incorporation reaction. In some embodiments, the nucleic acid analysis can be conducted at a single reaction site, or at a plurality of reaction sites in an array of reaction sites. Optionally, the array contains a plurality of reaction sites having about 1-100 million, or about 100-250 million, or about 200-500 million, or about 500-900 million, or more reaction sites. Optionally, each reaction site is in contact with, operatively coupled, or capacitively coupled to one or more sensors that are ion-sensitive FETs (isFETs) or chemically-sensitive FETs (chemFETs) sensors. Optionally, the reaction sites are in fluid communication with each other.Type: ApplicationFiled: March 18, 2016Publication date: May 3, 2018Applicants: Life Technologies Corporation, Life Technologies GmbHInventors: Wolfgang HINZ, Steven MENCHEN, Ronald GRAHAM, Peter VANDER HORN, Earl HUBBELL, Christian WOEHLER, Roman ROZHKOV, Barnett ROSENBLUM
-
Patent number: 9575069Abstract: Compositions, methods and kits for labeling proteins and uses in reporter systems for detecting, quantifying and/or characterizing analytes.Type: GrantFiled: November 8, 2013Date of Patent: February 21, 2017Assignee: APPLIED BIOSYSTEMS, LLCInventors: Steven Menchen, Joe Y. Lam, Zhaochun Ma, Khairuzzaman Bashar Mullah
-
Patent number: RE46683Abstract: Disclosed are methods and kits applicable to sequencing methods, such as Sanger dideoxy sequencing methods. The methods and kits disclosed utilize a cationically charged nucleic acid terminator in combination with a discriminatory polymerase.Type: GrantFiled: September 30, 2014Date of Patent: January 23, 2018Assignee: Applied Biosystems, LLCInventors: Barnett Rosenblum, Steven Menchen, Shaheer Khan, Paul Kenney