Abstract: The invention provides a method for obtaining a broadly neutralizing antibody (bNab), including screening memory B cell cultures from a donor PBMC sample for neutralization activity against a plurality of HIV-1 species, cloning a memory B cell that exhibits broad neutralization activity; and rescuing a monoclonal antibody from that memory B cell culture. The resultant monoclonal antibodies may be characterized by their ability to selectively bind epitopes from the Env proteins in native or monomeric form, as well as to inhibit infection of HIV-1 species from a plurality of clades. Compositions containing human monoclonal anti-HIV antibodies used for prophylaxis, diagnosis and treatment of HIV infection are provided. Methods for generating such antibodies by immunization using epitopes from conserved regions within the variable loops of gp120 are provided. Immunogens for generating anti-HIV1 bNAbs are also provided. Furthermore, methods for vaccination using suitable epitopes are provided.

Abstract: A safety marker includes an hollow frusto-conical body; a base having an bowl-shaped main portion and a resiliently flexible skirt for yieldably stabilizing the marker against tipping relative to a supportive surface, the body being connected in upstanding relation to an inner portion of the base and concentric therewith, the base being formed to clear the cone defined by the inside surface of the body, the bowl being sufficiently large relative to the body and the base being weighted sufficiently that the marker is self-righting, the skirt being sufficiently flexible to be deflected against the bowl for preserving the self-righting capability of the marker.