Patents by Inventor Steven R. Little

Steven R. Little has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20220296716
    Abstract: A method for treating an ocular disorder in a subject comprising administering a therapeutic agent-loaded carrier to an ocular site of the subject in need thereof, wherein the therapeutic agent loaded-carrier provides controlled delivery of the therapeutic agent under conditions suitable for recruiting regulatory T cells to an ocular region of interest or inducing regulatory T cells in an ocular region of interest.
    Type: Application
    Filed: June 7, 2022
    Publication date: September 22, 2022
    Applicant: University of Pittsburgh - Of the Commonwealth System of Higher Education
    Inventors: Steven R. Little, Michelle L. Guaragno, Andrew J. Glowacki, Morgan V. Fedorchak, Stephen C. Balmert
  • Patent number: 11413245
    Abstract: The present invention contemplates induction of immunological tolerance thereby providing permanent allograft acceptance. This method obviates the need for a lifelong regimen of immunosuppressive agents which can increase the risk of infection, autoimmunity, and cancer. Immunological tolerance is thought to be mediated by regulatory T lymphocytes (Treg cells) with immunosuppressive capabilities. A therapeutically relevant platform comprising artificial constructs are contemplated comprising numerous soluble and surface bound Treg cell stimulating factors that may induce tolerance following allograft transplantation. Such artificial constructs, being the size of a cell, have surface bound monoclonal antibodies specific to regulatory T-cell surface moieties and encapsulated soluble regulatory T-cell modulating factors.
    Type: Grant
    Filed: September 11, 2019
    Date of Patent: August 16, 2022
    Assignees: UNIVERSITY OF PITTSBURGH—OF THE COMMONWEALTH SYSTEM OF HIGHER EDUCATION, MASSACHUSETTS INSTITUTE OF TECHNOLOGY
    Inventor: Steven R. Little
  • Patent number: 11395853
    Abstract: A method for treating an ocular disorder in a subject comprising administering a therapeutic agent-loaded carrier to an ocular site of the subject in need thereof, wherein the therapeutic agent loaded-carrier provides controlled delivery of the therapeutic agent under conditions suitable for recruiting regulatory T cells to an ocular region of interest or inducing regulatory T cells in an ocular region of interest.
    Type: Grant
    Filed: June 20, 2017
    Date of Patent: July 26, 2022
    Assignee: University of Pittsburgh - Of the Commonwealth System of Higher Education
    Inventors: Steven R. Little, Michelle L. Guaragno, Andrew J. Glowacki, Morgan V. Fedorchak, Stephen C. Balmert
  • Publication number: 20220211632
    Abstract: A method for sustained delivery of an agent to an ocular organ in a subject, comprising topically delivering to the ocular surface a liquid thermoresponsive hydrogel comprising agent-loaded polymer microparticles, wherein the agent is sustainably released for a period of at least five days.
    Type: Application
    Filed: January 21, 2022
    Publication date: July 7, 2022
    Inventors: Morgan V. Fedorchak, Steven R. Little, Joel S. Schuman, Anthony Cugini
  • Publication number: 20220202705
    Abstract: A method for sustained delivery of an agent to an ocular organ in a subject, comprising topically delivering to the ocular surface a liquid thermoresponsive hydrogel comprising agent-loaded polymer microparticles, wherein the agent is an antibody, a fusion protein, a chemokine, an interleukin, a growth factor, albumin, immunoglobulin, an interferon, a peptide, stem cell-conditioned media, plasma or serum.
    Type: Application
    Filed: January 18, 2022
    Publication date: June 30, 2022
    Applicant: University of Pittsburgh - Of the Commonwealth System of Higher Education
    Inventors: Morgan V. Fedorchak, Steven R. Little, Joel S. Schuman
  • Patent number: 11318103
    Abstract: Methods for inhibiting tissue ossification or calcification in a subject, comprising administering a therapeutically effective amount of BMP I inhibitor-loaded microparticles to a subject in need thereof, wherein the administration provides local and sustained release of the BMP I inhibitor thereby inhibiting tissue ossification or calcification.
    Type: Grant
    Filed: November 29, 2018
    Date of Patent: May 3, 2022
    Assignees: University of Pittsburgh—Of the Commonwealth System of Higher Education, Ri.Med Foundation
    Inventors: Riccardo Gottardi, Peter Alexander, Patrick A. Bianconi, Steven R. Little
  • Publication number: 20220125729
    Abstract: A method for treating an ocular disorder in a subject comprising administering a therapeutic agent-loaded carrier to an ocular site of the subject in need thereof, wherein the therapeutic agent is a histone deactylase inhibitor.
    Type: Application
    Filed: January 7, 2022
    Publication date: April 28, 2022
    Applicant: University of Pittsburgh - Of the Commonwealth System of Higher Education
    Inventors: Steven R. Little, Michelle L. Ratay
  • Patent number: 11253480
    Abstract: A method for treating an ocular disorder in a subject comprising administering a therapeutic agent-loaded carrier to an ocular site of the subject in need thereof, wherein the therapeutic agent is a histone deactylase inhibitor.
    Type: Grant
    Filed: October 30, 2018
    Date of Patent: February 22, 2022
    Assignee: University of Pittsburgh—Of the Commonwealth System of Higher Education
    Inventors: Steven R. Little, Michelle L. Ratay
  • Patent number: 11246838
    Abstract: A method for sustained delivery of an agent to an ocular organ in a subject, comprising topically delivering to the ocular surface a liquid thermoresponsive hydrogel comprising agent-loaded polymer microparticles, wherein the agent is sustainably released for a period of at least five days.
    Type: Grant
    Filed: March 4, 2014
    Date of Patent: February 15, 2022
    Assignee: University of Pittsburgh—Of the Commonwealth System of Higher Education
    Inventors: Morgan Virginia Fedorchak, Steven R. Little, Joel Steven Schuman, Anthony Cugini
  • Publication number: 20210386825
    Abstract: The present invention provides microparticles that induce the migration of multipotent stem cells to the anatomical site of the microparticles. Various release profiles are demonstrated that depend upon the relative concentration of alginate in the chemokine-loaded microparticle. Local administration and/or intraarticular injection of the microparticles are useful in conditions such as osteoarthritis. Targeted systemic delivery of the alginate chemokine microparticles to distant anatomical sites subjected to autoimmune disease symptomology can be performed by encapsulation within liposomes having targeting ligands. Consequently, upon the creation of the appropriate chemokine gradient, multipotent stem cells will migrate to the distant anatomical site where the liposomes are attached.
    Type: Application
    Filed: August 30, 2021
    Publication date: December 16, 2021
    Applicants: UNIVERSITY OF PITTSBURGH - OF THE COMMONWEALTH SYSTEM OF HIGHER EDUCATION, Ri.MED Foundation
    Inventors: Steven R. Little, Riccardo Gottardi, Mintai Peter Hwang, Daniel DeSantis
  • Publication number: 20210379121
    Abstract: The present disclosure relates to probiotics and probiotic compositions having modified carbohydrate metabolism (e.g., modifying or metabolizing carbohydrates, or reduced glucose production), and thus regulating the absorption of the carbohydrates by the host subject. The present disclosure also relates to methods of making the probiotics and probiotic compositions. The present disclosure further relates to methods of regulating body weight in a subject, and methods of treating carbohydrate metabolism disorder, using the probiotics and probiotic compositions disclosed herein.
    Type: Application
    Filed: August 24, 2021
    Publication date: December 9, 2021
    Applicant: UNIVERSITY OF PITTSBURGH - OF THE COMMONWEALTH SYSTEM OF HIGHER EDUCATION
    Inventors: Abhinav Prakash Acharya, Steven R. Little
  • Publication number: 20210369649
    Abstract: This document provided ocular delivery systems for treatment of cystinosis as well as methods for making such ocular delivery systems and methods for using such ocular delivery systems. For example, ocular delivery systems designed to include spray-dried, cysteamine-loaded microparticles suspended in a thermoresponsive gel are provided herein.
    Type: Application
    Filed: November 18, 2019
    Publication date: December 2, 2021
    Inventors: Morgan Virginia Fedorchak, Steven R. Little, Joel S. Schuman
  • Publication number: 20210353716
    Abstract: Controlled release of VIP from PLGA microparticles was accomplished and varied through use of different polymer molecular sizes, addition of solutes to the inner aqueous phase, and use of our computer model. Released VIP from microparticles appeared to be bioactive and caused DCs to produce more CCL22 than DCs treated with blank particles at 7 and 24 hours. Additionally, DCs treated with VIP microparticle releasates recruited higher percentages of FoxP3+ T-cells in in vitro chemotaxis studies. Testing in a mouse model in vivo indicated that VIP microparticles have significant therapeutic potential to treat periodontal disease by reducing the bone loss in infected mice relative to the blank group.
    Type: Application
    Filed: June 29, 2021
    Publication date: November 18, 2021
    Applicant: UNIVERSITY OF PITTSBURGH - OF THE COMMONWEALTH SYSTEM OF HIGHER EDUCATION
    Inventors: Steven R. Little, Andrew Jason Glowacki
  • Patent number: 11129875
    Abstract: The present invention provides microparticles that induce the migration of multipotent stem cells to the anatomical site of the microparticles. Various release profiles are demonstrated that depend upon the relative concentration of alginate in the chemokine-loaded microparticle. Local administration and/or intraarticular injection of the microparticles are useful in conditions such as osteoarthritis. Targeted systemic delivery of the alginate chemokine microparticles to distant anatomical sites subjected to autoimmune disease symptomology can be performed by encapsulation within liposomes having targeting ligands. Consequently, upon the creation of the appropriate chemokine gradient, multipotent stem cells will migrate to the distant anatomical site where the liposomes are attached.
    Type: Grant
    Filed: January 7, 2019
    Date of Patent: September 28, 2021
    Assignees: UNIVERSITY OF PITTSBURGH—OF THE COMMONWEALTH SYSTEM OF HIGHER EDUCATION, RI.MED FOUNDATION
    Inventors: Steven R. Little, Riccardo Gottardi, Mintai Peter Hwang, Daniel DeSantis
  • Patent number: 11090363
    Abstract: Controlled release of VIP from PLGA microparticles was accomplished and varied through use of different polymer molecular sizes, addition of solutes to the inner aqueous phase, and use of our computer model. Released VIP from microparticles appeared to be bioactive and caused DCs to produce more CCL22 than DCs treated with blank particles at 7 and 24 hours. Additionally, DCs treated with VIP microparticle releasates recruited higher percentages of FoxP3+ T-cells in in vitro chemotaxis studies. Testing in a mouse model in vivo indicated that VIP microparticles have significant therapeutic potential to treat periodontal disease by reducing the bone loss in infected mice relative to the blank group.
    Type: Grant
    Filed: January 23, 2014
    Date of Patent: August 17, 2021
    Assignee: UNIVERSITY OF PITTSBURGH—OF THE COMMONWEALTH SYSTEM OF HIGHER EDUCATION
    Inventors: Steven R. Little, Andrew Jason Glowacki
  • Patent number: 11061033
    Abstract: A Fe(II)- and Ca+2-chelated alginate/gelatin conjugate.
    Type: Grant
    Filed: April 5, 2017
    Date of Patent: July 13, 2021
    Assignee: University of Pittsburgh—Of the Commonwealth System of Higher Education
    Inventors: Abhinav Acharya, Steven R. Little, Tatum V. Tarin
  • Publication number: 20210015770
    Abstract: Methods for treating an ear condition in a subject, comprising topically administering to the ear of the subject in need thereof a composition comprising: (i) an anti-infective agent-loaded biodegradable polymer microspheres; and (ii) a thermoresponsive hydrogel.
    Type: Application
    Filed: December 10, 2018
    Publication date: January 21, 2021
    Applicant: UNIVERSITY OF PITTSBURGH - OF THE COMMONWEALTH SYSTEM OF HIGHER EDUCATION
    Inventors: Morgan V. Fedorchak, Steven R. Little, Joel S. Schuman
  • Publication number: 20200405644
    Abstract: The absence of regulatory T cells (Treg) may underlie disorders including but not limited to autoimmunity, dermatitis, periodontitis and even transplant rejection. Enhancing local numbers of Treg through in situ Treg expansion or induction is contemplated herein as a treatment option for these disorders. Current methods for in vivo Treg expansion are not Treg specific and are associated with many adverse side-effects. The data presented herein provides in vitro testing of a Treg-inducing microparticle providing a predictable controlled release for combinations of cytokines and drugs (e.g., IL-2, TGF-?, and/or rapamycin) resulting in targeted Treg migration. These controlled release microparticles are also capable of inducing FoxP3+ Treg in human cells in vitro suggesting that these compositions be developed into an in vivo Treg induction and expansion therapy.
    Type: Application
    Filed: July 29, 2020
    Publication date: December 31, 2020
    Applicant: UNIVERSITY OF PITTSBURGH - OF THE COMMONWEALTH SYSTEM OF HIGHER EDUCATION
    Inventors: Steven R. Little, Giorgio Raimondi, Angus W. Thomson, Siddharth Jhunjhunwala
  • Publication number: 20200390829
    Abstract: The present disclosure relates to probiotics and probiotic compositions having modified energy metabolisms (e.g., modifying, metabolizing, or storing energy molecules, e.g., lipids, before the energy molecules are absorbed by a host subject), and thus modulating the absorption of the energy molecules by the host subject. The present disclosure also relates to methods of making such probiotics and probiotic compositions. The present disclosure further relates to methods of regulating (e.g., maintaining or reducing) body weight in a subject (e.g., a subject having a healthy BMI, a subject having an overweight BMI, a subject having an obese BMI), using the probiotics and probiotic compositions disclosed herein.
    Type: Application
    Filed: August 27, 2020
    Publication date: December 17, 2020
    Applicant: UNIVERSITY OF PITTSBURGH - OF THE COMMONWEALTH SYSTEM OF HIGHER EDUCATION
    Inventors: Abhinav Prakash Acharya, Steven R. Little
  • Publication number: 20200360518
    Abstract: A method for making a modified release composition, comprising: selecting a desired active agent and polymer matrix for formulating into a modified release composition; assessing degradation effect on release of the active agent from the composition including plotting polymer molecular weight (Mwr) at onset of active agent release vs. active agent molecular weight (MwA); predicting performance of multiple potential formulations for the composition based on the degradation assessment and average polymer matrix initial molecular weight (Mwo) to define a library of building blocks; determining the optimal ratio of the building blocks to satisfy a specified release profile; and making a modified release composition based on the optimal ratio determination.
    Type: Application
    Filed: May 4, 2020
    Publication date: November 19, 2020
    Applicant: University of Pittsburgh - Of the Commonwealth System of Higher Education
    Inventors: Steven R. Little, Sam N. Rothstein