Abstract: A system and method of saving deal offers to be applied at a point-of-sale (POS) of a retail store may include receiving, by a mobile device via a communications network, multiple advertisements inclusive of selectable deal offers of products being sold at the retail store for a user to select. Responsive to a user selecting to view an advertisement of a product on the mobile device, the deal offer of the product to be presented to the POS to be applied to a cost of the product may be automatically stored. Responsive to a check out request at the mobile device, data inclusive of the deal offer to be presented to the POS to apply the deal offer to the cost of the product to which the deal offer is associated may be generated.

Abstract: In one aspect, the present invention provides an in situ process of simultaneously detecting a specific predetermined nucleic acid sequence and a specific predetermined cellular antigen in the same cell. In accordance with that process, the antigen is labeled with a biotin- or DNP-tagged antibody that specifically immunoreacts with the antigen, the specific nucleic acid sequences in the cell are amplified, the amplified nucleic acid sequences are labeled with a fluorescently-tagged nucleic acid probe that specifically hybridizes to the amplified nucleic acid sequences, and the labeled nucleic acid sequences and labeled cellular antigen are detected.

Abstract: The field of this invention is the detection and isolation of specific amplified DNA sequences by flow cytometry. More particularly, this invention relates to the detection of these specific amplified DNA sequences in cells so as to allow quantitation of viral burden of patients infected with a virus. The method is particularly adapted to detection of HIV-1 proviral DNA sequences and the assessment of activity of the virus in a cell.