Abstract: Provided are compositions and methods for used in solubilizing, stabilizing and expressing proteins. The proteins are fusion proteins that contain a protein of interest. The fusion proteins contain segments of Ribose Binding Protein (RBP) or Maltose Binding Protein (MBP). The fusion proteins can have the RBP or MBP segments flanking the target protein, and the RBP or MBP segments can be in the fusion protein in the same orientation as they normally occur (except for being interrupted by the target protein) or the segments can be permuted. Novel segments of the RBP and MBP are provided and result in improved expression and/or solubility of the proteins. Some examples include one or a combination of two complete or partial Histidine tags. Some examples allow for the target protein to be separated from all or a part of the fusion protein such as by enzymatic or non-enzymatic cleavage.

Abstract: Provided are compositions and methods for enhancing recombinant protein production. The compositions and methods involve use of Ribose Binding Protein (RBP) as a segment of a fusion polypeptide, whereby the RBP segment enhances production of the fusion protein. The fusion proteins contain the RBP sequentially in a single fusion protein with a polypeptide for which enhanced expression is desired. Recombinant expression vectors encoding the fusion proteins that contain and RBP segment are included, as are cells that contain the expression vectors. Methods for separating fusion proteins and for liberating a polypeptide segment that is part of the fusion protein are also provided.

Abstract: The invention provides compounds of formula (I): and salts thereof wherein ring A, R2, HET, X, n, and R3 have any of the meanings described in the specification, as well as compositions comprising such compounds and salts, and methods for treating cancer using such compounds and salts.

Abstract: The invention provides complexes of Zn2+ of formulae (la) and (IIa) that are useful for treating cancer, as well as compositions and kits comprising such complexes.

Abstract: The invention provides organic complexes of Zn2+ that are useful for treating cancer, as well as compositions and kits comprising such complexes, and intermediate monomer compounds that are useful for the preparation of such complexes.

Abstract: The invention provides compounds of formula I and II and salts thereof, wherein R1, R2, Y, R3, and R4 have any of the meanings described in the specification, as well as compositions comprising such compounds and salts, and methods for treating cancer using such compounds and salts.

Abstract: Provided are compositions and methods for used in solubilizing, stabilizing and expressing proteins. The proteins are fusion proteins that contain a protein of interest. The fusion proteins contain segments of Ribose Binding Protein (RBP) or Maltose Binding Protein (MBP). The fusion proteins can have the RBP or MBP segments flanking the target protein, and the RBP or MBP segments can be in the fusion protein in the same orientation as they normally occur (except for being interrupted by the target protein) or the segments can be permuted. Novel segments of the RBP and MBP are provided and result in improved expression and/or solubility of the proteins. Some examples include one or a combination of two complete or partial Histidine tags. Some examples allow for the target protein to be separated from all or a part of the fusion protein such as by enzymatic or non-enzymatic cleavage.

Abstract: The invention provides organic complexes of Zn2+ that are useful for treating cancer, as well as compositions and kits comprising such complexes, and intermediate monomer compounds that are useful for the preparation of such complexes.

Abstract: Provided are compositions and methods for enhancing recombinant protein production. The compositions and methods involve use of Ribose Binding Protein (RBP) as a segment of a fusion polypeptide, whereby the RBP segment enhances production of the fusion protein. The fusion proteins contain the RBP sequentially in a single fusion protein with a polypeptide for which enhanced expression is desired. Recombinant expression vectors encoding the fusion proteins that contain and RBP segment are included, as are cells that contain the expression vectors. Methods for separating fusion proteins and for liberating a polypeptide segment that is part of the fusion protein are also provided.

Abstract: The invention provides organic complexes of Zn2+ of formula (I) that are useful for treating cancer, as well as compositions and kits comprising such complexes, and intermediate monomer compounds that are useful for the preparation of such complexes.

Abstract: Provided are compositions and methods for enhancing recombinant protein production. The compositions and methods involve use of Ribose Binding Protein (RBP) as a segment of a fusion polypeptide, whereby the RBP segment enhances production of the fusion protein. The fusion proteins contain the RBP sequentially in a single fusion protein with a polypeptide for which enhanced expression is desired. Recombinant expression vectors encoding the fusion proteins that contain and RBP segment are included, as are cells that contain the expression vectors. Methods for separating fusion proteins and for liberating a polypeptide segment that is part of the fusion protein are also provided.

Abstract: Provided are compositions and methods for enhancing recombinant protein production. The compositions and methods involve use of Ribose Binding Protein (RBP) as a segment of a fusion polypeptide, whereby the RBP segment enhances production of the fusion protein. The fusion proteins contain the RBP sequentially in a single fusion protein with a polypeptide for which enhanced expression is desired. Recombinant expression vectors encoding the fusion proteins that contain and RBP segment are included, as are cells that contain the expression vectors. Methods for separating fusion proteins and for liberating a polypeptide segment that is part of the fusion protein are also provided.

Abstract: The invention provides compounds of formula (I): and salts thereof wherein ring A, R2, HET, X, n, and R3 have any of the meanings described in the specification, as well as compositions comprising such compounds and salts, and methods for treating cancer using such compounds and salts.

Abstract: The invention provides organic complexes of Zn2+ of formula (I) that are useful for treating cancer, as well as compositions and kits comprising such complexes, and intermediate monomer compounds that are useful for the preparation of such complexes.

Abstract: The invention provides complexes of Zn2+ of formulae (la) and (IIa) that are useful for treating cancer, as well as compositions and kits comprising such complexes.

Abstract: The invention provides compounds of formula I and II and salts thereof, wherein R1, R2, Y, R3, and R4 have any of the meanings described in the specification, as well as compositions comprising such compounds and salts, and methods for treating cancer using such compounds and salts.

Abstract: The invention is a fusion protein, embodying a mutually exclusive folding domain molecular switch, wherein the free energy released by folding of a first domain of the fusion protein drives an unfolding of a second domain of the fusion protein, and vice versa. The fusion protein is engineered so that folding the first domain unfolds the second domain, and vice versa, making the folded and unfolded states of the domains mutually exclusive. This is accomplished by insertion of an insert protein GCN4 into a surface loop of a target, protein barnase, subject to die topological design, criterion that the N—C terminal length of GCN4 be at least two-times greater than the C?-C? length of a surface loop of barnase. In the absence of the ligand AP-1, barnase is more stable and is folded and active. The presence of AP-1 induces folding of GCN4, forcibly unfolding and inactivating barnase.