Patents by Inventor Stratis Avrameas
Stratis Avrameas has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 7628987Abstract: The present invention provides novel polypeptides which can effectively penetrate into cells thereby transporting a substance of interest into the cells.Type: GrantFiled: June 13, 2003Date of Patent: December 8, 2009Assignees: Institut Pasteur, Universite Pierre et Marie CurieInventors: Therese Ternynck, Alexandre Avrameas, Gerard Buttin, Stratis Avrameas, Marie-Francoise Saron, Bruno Blondel, Therese Couderc, Susan Michelson, Donato Zipeto
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Publication number: 20070098741Abstract: The present invention provides novel polypeptides which can effectively penetrate into cells thereby transporting a substance of interest into the cells.Type: ApplicationFiled: July 10, 2006Publication date: May 3, 2007Applicants: INSTITUT PASTEUR, Universite Pierre et Marie CurieInventors: Therese Ternynck, Alexandre Avrameas, Gerard Buttin, Stratis Avrameas, Marie-Francoise Saron, Bruno Blondel, Therese Couderc, Susan Michelson, Donato Zipeto
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Publication number: 20040259822Abstract: A product for coupling a biologically active principle with an immunovector, characterized in that the immunovector is capable of enabling the biologically active principle to be internalized into eukaryotic cells, and in that said immunovector has an affinity for the cell DNA to such an extent that it can transfer the biologically active principle into or to the immediate vicinity of the cell nuclei.Type: ApplicationFiled: June 20, 2003Publication date: December 23, 2004Applicants: INSTITUT PASTEUR, Universite Pierre et Marie CurieInventors: Stratis Avrameas, Gerard Buttin, Therese Ternynck, Faridabano Nato, Alexandre Avrameas
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Publication number: 20030206900Abstract: The present invention provides novel polypeptides which can effectively penetrate into cells thereby transporting a substance of interest into the cells.Type: ApplicationFiled: June 13, 2003Publication date: November 6, 2003Applicants: INSTITUT PASTEUR, Universite Pierre et Marie CurieInventors: Therese Ternynck, Alexandre Avrameas, Gerard Buttin, Stratis Avrameas, Marie-Francoise Saron, Bruno Blondel, Therese Couderc, Susan Michelson, Donato Zipeto
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Patent number: 6635248Abstract: The present invention provides novel polypeptides which can effectively penetrate into cells thereby transporting a substance of interest into the cells.Type: GrantFiled: February 4, 2000Date of Patent: October 21, 2003Assignees: Institut Pasteur, Universite Pierre et Marie CurieInventors: Therese Ternynck, Alexandre Avrameas, Gerard Buttin, Stratis Avrameas, Marie-Francoise Saron, Bruno Blondel, Therese Couderc, Susan Michelson, Donato Zipeto
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Patent number: 6608034Abstract: A product for coupling a biologically active principle with an immunovector, characterized in that the immunovector is capable of enabling the biologically active principle to be internalized into eukaryotic cells, and in that said immunovector has an affinity for the cell DNA to such an extent that it can transfer the biologically active principle into or to the immediate vicinity of the cell nuclei.Type: GrantFiled: April 28, 1998Date of Patent: August 19, 2003Assignees: Institut Pasteur, Universite Pierre et Marie Curie (Paris VI)Inventors: Stratis Avrameas, Gerard Buttin, Therese Ternynck, Faridabano Nato, Alexandre Avrameas
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Method of detecting and characterizing a nucleic acid or reactant for the application of this method
Patent number: 5955262Abstract: Method for detecting the possible presence of a DNA fragment, notably of a gene, in the midst of a complex sample of nucleic acids.It comprises the hybridization of the sought fragment with a RNA probe, this being, prior or subsequent to the hybridization reaction, modified by an enzyme.Application to seeking of particular genes or DNA fragments in the midst of a biological sample.Type: GrantFiled: September 8, 1992Date of Patent: September 21, 1999Assignee: Institut PasteurInventors: Philippe Kourilsky, Stratis Avrameas, Brigitte Cami Contamine, Jean-Luc Guesdon -
Patent number: 5876928Abstract: Method for detecting the possible presence of a DNA fragment, notably of a gene, in the midst of a complex sample of nucleic acids.It comprises the hybridization of the sought fragment with a RNA probe, this being, prior or subsequent to the hybridization reaction, modified by an enzyme.Application to seeking of particular genes or DNA fragments in the midst of a biological sample.Type: GrantFiled: June 6, 1995Date of Patent: March 2, 1999Assignee: Institut PasteurInventors: Philippe Kourilsky, Stratis Avrameas, Brigitte Cami, born Contamine, Jean-Luc Guesdon
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Patent number: 5605800Abstract: Detection methods and compositions of matter comprising an enzyme coupled to a nucleic acid are disclosed. A complex of the enzyme-nucleic acid hybridized to a nucleic acid to be detected is also disclosed. The nucleic acid is detected by measuring the activity of the enzyme.Type: GrantFiled: June 6, 1995Date of Patent: February 25, 1997Assignee: Institut PasteurInventors: Philippe Kourilsky, Stratis Avrameas, Brigitte Cami, born Contamine, Jean-Luc Guesdon
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Patent number: 4925921Abstract: This process involves the use of benzoquinone as cross-linking agent in large excess compared with the substance to be activated. The activation reaction is realized in a homogeneous liquid medium. Said process making it possible for example to couple antibodies to enzymes for determining or detecting said antibodies.The process can be used for the determination of antitetanic antibodies in human serum.Type: GrantFiled: March 24, 1986Date of Patent: May 15, 1990Assignee: Etablissement Declare d'Utilite Publique Dit. Institut PasteurInventors: Stratis Avrameas, Therese M. F. Ternynck
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Patent number: 4900661Abstract: This method for immunological determination of amines in biological media consists of: (a) reacting a quinone (in particular, p-benzoquinone) with the amine (for example histamine) present in the test sample; (b) introducing into the medium a monoclonal antibody which specifically recognizes the amine-quinone reaction product formed; and (c) determining one of the forms of the monoclonal antibody present. When the quinone is used in the immobilized state, the monoclonal antibody present is determined in its complexed form with the amine-quinone reaction product (direct type method); when the quinone is used in the free state, the monoclonal antibody is determined in its free form (enzymoimmunometric method or competitive immunoenzymatic method).Type: GrantFiled: February 12, 1986Date of Patent: February 13, 1990Assignees: Institut Pasteur Paris, Centre National de la Recherche Scientifique (CNRS)Inventors: Jean-Luc Guesdon, Stratis Avrameas, Jean-Claude Mazie
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Patent number: 4729961Abstract: A process for the detection and assay of a biological substance by erythroadsorption by immobilizing a substance having a binding affinity for the biological substance to be assayed; incubating the immobilized substance with a liquid medium containing the biological substance to be assayed, forming a fixed substance; incubating the fixed substance with a coupling product comprising a specific ligand and a second ligand which binds with erythrocytes; adding erythrocytes; and determining the amount of erythrocytes bound to the coupling product. The determination of the amount of bound erythrocytes can be made visually with the naked eye or determined by lysis of the erythrocytes bound to the coupling product and quantitatively measuring the released hemoglobin. The specific ligand can be an antibody or an antigen and the second ligand capable of coupling erythrocytes can, for example, be red blood cell antibodies.Type: GrantFiled: December 11, 1985Date of Patent: March 8, 1988Assignee: Institut PasteurInventors: Stratis Avrameas, Jean-Luc Guesdon
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Patent number: 4668637Abstract: A process for the detection of a biological substance immobilized on a support including(1) incubating, after washing, the substance immobilized on a support with the product of coupling of a specific ligand with a ligand capable of reacting with erythrocytes;(2) adding erythrocytes;(3) immersing the whole in a solution of a fixing agent and(4) measuring the erythroadsorption, after having turned the support over in order to permit the removal of the red cells which have not reacted.Type: GrantFiled: May 30, 1984Date of Patent: May 26, 1987Assignee: Institut PasteurInventors: Jean-Luc Guesdon, Stratis Avrameas
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Patent number: 4592998Abstract: This process involves the use of benzoquinone as cross-linking agent in large excess compared with the substance to be activated. The activation reaction is realized in a homogeneous liquid medium. Said process making it possible for example to couple antibodies to enzymes for determining or detecting said antibodies. The process can be used for the determination of antitetanic antibodies in human serum.Type: GrantFiled: December 15, 1983Date of Patent: June 3, 1986Assignee: Etablissement Declare d'Utilite Publique Dit: Institut PasteurInventors: Stratis Avrameas, Therese M. F. Ternynck
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Method of detecting and characterizing a nucleic acid or reactant for the application of this method
Patent number: 4581333Abstract: Method for detecting the possible presence of a DNA fragment, notably of a gene, in the midst of a complex sample of nucleic acids.It comprises the hybridization of the sought fragment with a RNA probe, this being, prior or subsequent to the hybridization reaction, modified by an enzyme.Application to seeking of particular genes or DNA fragments in the midst of a biological sample.Type: GrantFiled: April 29, 1982Date of Patent: April 8, 1986Assignee: Institut PasteurInventors: Philippe Kourilsky, Stratis Avrameas, Brigitte Cami nee Contamine, Jean-Luc Guesdon -
Patent number: 4526871Abstract: The present invention relates to a conjugate of a specific ligand and a lectin, by covalent bonding.The product of the invention may, for example, be the conjugate of Concanavalin A and an antigen, a hapten, an antibody, a hormone or its receptor, an enzyme or its inhibitor or a lectin. Coupling being effected by means of glutaraldehyde or p-benzoquinone.Type: GrantFiled: May 20, 1981Date of Patent: July 2, 1985Assignee: Institut PasteurInventors: Stratis Avrameas, Jean-Luc Guesdon
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Patent number: 4464468Abstract: A solution of an active protein substance and an inactive protein substance is reacted with a cross-linking agent, optionally in the presence of an inert carrier, under cross-linking conditions to produce articles comprising both active and inactive protein substances. The active protein substance comprises up to about 20 percent, e.g. from 1 to 20 percent by weight, based on the final weight of the total protein substance, whereas the cross-linking agent comprises from 0.5 to 8 percent by weight, based on the weight of the total treated mixture. The obtained articles are in the form of a solution or a suspension in aqueous medium, in the form of a film, in the form of a membrane, in the form of a fabric, in the form of a porous material, or in the form of a mass, such as granules, pills or tablets.Type: GrantFiled: April 16, 1982Date of Patent: August 7, 1984Assignee: Agence Nationale de Valorisation de la Recherche (ANVAR)Inventors: Stratis Avrameas, Georges Broun, Eric Selegny, Daniel Thomas
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Patent number: 4241176Abstract: The invention relates to a magnetic gel which is useful for immunoezymatic determinations.Said gel consisting, for example, of polyacrylamideagarose and magnetic particles, coupled with an antibody, can be used for rapid determinations of the antigens contained in a biological liquid, the presence of magnetic particles permitting rapid separation of the gel from the reaction medium by means of a magnetic field and considerably shortening the time required for determinations.Type: GrantFiled: July 28, 1978Date of Patent: December 23, 1980Assignee: Etablissement declare d'Utilite Publique diti Institut PasteurInventors: Stratis Avrameas, Jean-Luc Guesdon
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Patent number: 4193982Abstract: A process for coupling biological substances by covalent bonds that involves the use of benzoquinone as cross-linking agent in large excess compared with the substance to be activated. The activation reaction is realized in a homogeneous liquid medium. The process makes it possible, for example, to couple antibodies to enzymes for determining or detecting said antibodies.The process can be used for the determination of antitetanic antibodies in human serum.Type: GrantFiled: December 1, 1976Date of Patent: March 18, 1980Assignee: Etablissement DeClare d'Utilite Publique dit: Institut PasteurInventors: Stratis Avrameas, Therese M. F. Ternynck
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Patent number: 4004979Abstract: Articles containing active proteins, such as enzymes, crosslinked to an inactive protein are prepared by dispersing and reacting in a solvent an active protein and an inactive protein simultaneously with a crosslinking agent to crosslink the active and inactive proteins together. The degree of crosslinking can be controlled by introducing tris (hydroxymethyl) aminomethane in an amount sufficient to stop chain growth. Optionally, an inert carrier may be present during crosslinking. The resultant crosslinked active and inactive proteins can be in various forms such as solutions, suspensions, gels, films, membranes, granules, pills or tablets.Type: GrantFiled: January 15, 1975Date of Patent: January 25, 1977Assignee: Agence Nationale de Valorisation de la Recherche (ANVAR)Inventors: Stratis Avrameas, Georges Broun, Eric Selegny, Daniel Thomas