Abstract: A system and method for maintaining the vitality of an organ through negative pressure ventilation and perfusion. The system includes fluidically coupled components: an organ enclosure, a diaphragm enclosure, an actuator/pump, a perfusion system, and a reservoir. The actuator can displace a precise amount of a working fluid that displaces that precise amount of a sterile support fluid. The sterile fluid travels between the diaphragm enclosure and the organ enclosure, thereby ventilating the organ within the organ chamber. The perfusion system circulates a perfusate through the organ.

Abstract: A system and method for growing and maintaining biological material including producing a protein associated with the tissue, selecting cells associated with the tissue, expanding the cells, creating at least one tissue bio-ink including the expanded cells, printing the at least one tissue bio-ink in at least one tissue growth medium mixture, growing the tissue from the printed at least one tissue bio-ink, and maintaining viability of the tissue.

Abstract: A configurable system for repeatably performing processes related to tissue growth in a controlled environment, possibly part of an industrial production line. The system can accommodate various sizes and shapes of culture vessels, and can maintain the cells at a desired temperature in the culture vessels, thus enabling a plug and play system that can produce consistent and repeatable results. The system includes gas management, fluid management, and control of multiple processes simultaneously, and can be automatically operably coupled with a variety of supporting technologies that can enable tissue-related processes. The system can communicate with supporting technologies upstream and downstream on a manufacturing line, enabling fully automated process control, centralized data historization, and centralized control.

Abstract: System and method for exercising cells and/or tissue in a sealed bioreactor. System includes a cartridge having a stretching means and a means to retain cells on a scaffold mounted within the cartridge during seeding. System includes a bioreactor including a sealed enclosure, the sealed enclosure housing the scaffold and the cartridge, the bioreactor enabling uniform seeding of the scaffold with the cells, the seeding occurring within the sealed enclosure to protect from environmental contamination. The bioreactor system provides a means to supply fresh media to the cells and/or tissue, and a means to remove waste products.

Abstract: A system and method for growing and maintaining biological material including producing a protein associated with the tissue, selecting cells associated with the tissue, expanding the cells, creating at least one tissue bio-ink including the expanded cells, printing the at least one tissue bio-ink in at least one tissue growth medium mixture, growing the tissue from the printed at least one tissue bio-ink, and maintaining viability of the tissue.

Abstract: A system and method for growing and maintaining biological material including producing a protein associated with the tissue, selecting cells associated with the tissue, expanding the cells, creating at least one tissue bio-ink including the expanded cells, printing the at least one tissue bio-ink in at least one tissue growth medium mixture, growing the tissue from the printed at least one tissue bio-ink, and maintaining viability of the tissue.

Abstract: A system and method for automated production of a viable cell culture on a mesh supported membrane lattice that is suitable for therapeutic implantation in connection with regenerative cell therapy. At least one bioreactor vial is configured to be supported and received by an automated handling and processing system. The bioreactor vial has a reactor well therein into which a mesh-supported submicron parylene-C membrane (MSPM) scaffold is received. Various support fluids are added and subsequently RPE cells are seeded onto the MSPM. The RPE cells form a culture of monolayer of hexagonally shaped RPE cells that is adhered to the MSPM which is suitable for subsequent transplantation into an eye in order to develop in a manner that supports and maintains the photoreceptors of the retina. The system is preferably automated and configured to process multiple bioreactors simultaneously.

Abstract: Manufacturing system and method for creating multiple tissue constructs from cells. System can include a thaw subsystem (if the cells are provided in a frozen state), an expansion subsystem, a concentration subsystem, and a tissue maturation subsystem. Each of these subsystems is modular and can be reconfigured, and the process can be repeated depending on the specific tissue process being implemented. Multiple tissue types can be combined in multiple bioreactors. The activities of multiple bioreactors can be coordinated and controlled in an automated manner by a supervisor controller. The supervisor controller can receive user input at the start of the process, and can manage the process henceforth, alerting the user if user actions are required.

Abstract: A system and method for growing and maintaining biological material including producing a protein associated with the tissue, selecting cells associated with the tissue, expanding the cells, creating at least one tissue bio-ink including the expanded cells, printing the at least one tissue bio-ink in at least one tissue growth medium mixture, growing the tissue from the printed at least one tissue bio-ink, and maintaining viability of the tissue.

Abstract: A system and method for growing and maintaining biological material including producing a protein associated with the tissue, selecting cells associated with the tissue, expanding the cells, creating at least one tissue bio-ink including the expanded cells, printing the at least one tissue bio-ink in at least one tissue growth medium mixture, growing the tissue from the printed at least one tissue bio-ink, and maintaining viability of the tissue.

Abstract: A system and method for growing and maintaining biological material including producing a protein associated with the tissue, selecting cells associated with the tissue, expanding the cells, creating at least one tissue bio-ink including the expanded cells, printing the at least one tissue bio-ink in at least one tissue growth medium mixture, growing the tissue from the printed at least one tissue bio-ink, and maintaining viability of the tissue.

Abstract: A system and method for growing and maintaining biological material including producing a protein associated with the tissue, selecting cells associated with the tissue, expanding the cells, creating at least one tissue bio-ink including the expanded cells, printing the at least one tissue bio-ink in at least one tissue growth medium mixture, growing the tissue from the printed at least one tissue bio-ink, and maintaining viability of the tissue.

Abstract: A system and method for tissue perfusion to assess, maintain, mature, and possibly rehabilitate the tissue. The system of the present teachings includes a tissue enclosure having a fluid reservoir. Pumps, valves, and a controller move perfusate through the tissue. The system includes features to assist in monitoring the health of the tissue, and a removable tray to facilitate moving the tissue from a point of origin to the tissue enclosure. The system moves perfusate to and through the tissue, and provides nutrition to the tissue. The system includes an output flow rate/volume sensor, at least one infusion pump, disposable and durable parts, and a sensor suite.

Abstract: System and method for exercising cells and/or tissue in a sealed bioreactor. System includes a cartridge having a stretching means and a means to retain cells on a scaffold mounted within the cartridge during seeding. System includes a bioreactor including a sealed enclosure, the sealed enclosure housing the scaffold and the cartridge, the bioreactor enabling uniform seeding of the scaffold with the cells, the seeding occurring within the sealed enclosure to protect from environmental contamination. The bioreactor system provides a means to supply fresh media to the cells and/or tissue, and a means to remove waste products.

Abstract: Manufacturing system and method for creating multiple tissue constructs from cells. System can include a thaw subsystem (if the cells are provided in a frozen state), an expansion subsystem, a concentration subsystem, and a tissue maturation subsystem. Each of these subsystems is modular and can be reconfigured, and the process can be repeated depending on the specific tissue process being implemented. Multiple tissue types can be combined in multiple bioreactors. The activities of multiple bioreactors can be coordinated and controlled in an automated manner by a supervisor controller. The supervisor controller can receive user input at the start of the process, and can manage the process henceforth, alerting the user if user actions are required.

Abstract: A system and method to enable treatment through cell therapy. The system can enable cell injection such as, for example, injecting beta cells/islets, cartilage cells, fat cells, and others. The system and method ensure that the delivery rate and delivered volume of the material pumped through a tube and into the injection needle is consistent over the course of the start-operation-stop process from run to run. The system and method can automatically stop delivery and alarm if an occlusion is detected during delivery.

Abstract: A system and method for maintaining the vitality of an organ through negative pressure ventilation and perfusion. The system includes fluidically coupled components: an organ enclosure, a diaphragm enclosure, an actuator/pump, a perfusion system, and a reservoir. The actuator can displace a precise amount of a working fluid that displaces that precise amount of a sterile support fluid. The sterile fluid travels between the diaphragm enclosure and the organ enclosure, thereby ventilating the organ within the organ chamber. The perfusion system circulates a perfusate through the organ.

Abstract: A system and method for growing and maintaining biological material including producing a protein associated with the tissue, selecting cells associated with the tissue, expanding the cells, creating at least one tissue bio-ink including the expanded cells, printing the at least one tissue bio-ink in at least one tissue growth medium mixture, growing the tissue from the printed at least one tissue bio-ink, and maintaining viability of the tissue.

Abstract: A configurable system for repeatably performing processes related to tissue growth in a controlled environment, possibly part of an industrial production line. The system can accommodate various sizes and shapes of culture vessels, and can maintain the cells at a desired temperature in the culture vessels, thus enabling a plug and play system that can produce consistent and repeatable results. The system includes gas management, fluid management, and control of multiple processes simultaneously, and can be automatically operably coupled with a variety of supporting technologies that can enable tissue-related processes. The system can communicate with supporting technologies upstream and downstream on a manufacturing line, enabling fully automated process control, centralized data historization, and centralized control.

Abstract: A system and method for growing and maintaining biological material including producing a protein associated with the tissue, selecting cells associated with the tissue, expanding the cells, creating at least one tissue bio-ink including the expanded cells, printing the at least one tissue bio-ink in at least one tissue growth medium mixture, growing the tissue from the printed at least one tissue bio-ink, and maintaining viability of the tissue.