Abstract: The disclosure provides compositions and methods for high-throughput screening a plurality of transposases to identify rare mutations that affect desired features of the transposase. Compositions of the disclosures are provided that facilitate the high-throughput screening methods of the disclosure.

Abstract: A zonal isolation device in the form of an active external casing packer is provided which includes a tubular section such as a casing or liner and at least one sleeve member positioned on the exterior of the casing or liner and sealed thereto. The method detailed herein provides zonal isolation by use of a pair of spaced apart sleeve members during a frac operation where frac fluid is supplied to a zone (in between the pair of sleeve members) requiring to be frac'd, where the frac pressure acts not only on the outside of the zonal isolation device but also on the interior of the sleeve member to enhance the seal provided thereby.

Abstract: A zonal isolation device in the form of an active external casing packer is provided which includes a tubular section such as a casing or liner and at least one sleeve member positioned on the exterior of the casing or liner and sealed thereto. The method detailed herein provides zonal isolation by use of a pair of spaced apart sleeve members during a frac operation where frac fluid is supplied to a zone (in between the pair of sleeve members) requiring to be frac'd, where the frac pressure acts not only on the outside of the zonal isolation device but also on the interior of the sleeve member to enhance the seal provided thereby.

Abstract: A zonal isolation device (10; 20; 30; 300; 400; 500; 600) in the form of an active external casing packer is provided which includes a tubular section such as a casing or liner (1; 21; 31; 171; 203) arranged to be run into and secured within a larger diameter generally cylindrical structure such as an open borehole (163; 213). At least one sleeve member (3; 23; 33; 43; 173) is positioned on the exterior of the casing or liner and is sealed thereto and at least two deformable band members (201; 401, 403; 501) are provided around and are bonded to the outer circumference of the sleeve member. The sleeve member and the at least two deformable band members is/are expanded into place against the inner circumference of the open borehole by increasing the pressure within the sleeve member.

Abstract: A zonal isolation device (10; 20; 30; 300; 400; 500; 600) in the form of an active external casing packer is provided which includes a tubular section such as a casing or liner (1; 21; 31; 171; 203) arranged to be run into and secured within a larger diameter generally cylindrical structure such as an open borehole (163; 213). At least one sleeve member (3; 23; 33; 43; 173) is positioned on the exterior of the casing or liner and is sealed thereto and at least two deformable band members (201; 401, 403; 501) are provided around and are bonded to the outer circumference of the sleeve member. The sleeve member and the at least two deformable band members is/are expanded into place against the inner circumference of the open borehole by increasing the pressure within the sleeve member.

Abstract: A method of forming recesses in a substrate such as a capacitor so as to increase the surface area thereof and therefore the charge storage capacity of the capacitor. This is accomplished by utilizing a micro mask formed by agglomeration on the surface of the substrate. The agglomerated material, such as gold, titanium nitride or titanium silicide, is used as a mask for selectively etching the substrate to form recesses therein. Alternatively, an oxide transfer mask can be utilized with the agglomerated material micro mask to etch the substrate.