Patents by Inventor Stuart Builder

Stuart Builder has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Refolding of polypeptides like recombinant insulin-like growth factor IGF-I
    Patent number: 5808006
    Abstract: A composition is provided comprising about 0.1 to 15 mg/ml of a polypeptide in a buffer having a pH of about 7-12 comprising about 5-40% (v/v) of an alcoholic or polar aprotic solvent, about 0.2 to 3M of an alkaline earth, alkali metal, or ammonium salt, about 0.1 to 9M of a chaotropic agent, and about 0.01 to 15 .mu.M of a copper or manganese salt. The buffer is suitably used in a method for refolding improperly folded polypeptides.
    Type: Grant
    Filed: October 11, 1994
    Date of Patent: September 15, 1998
    Assignee: Genentech, Inc.
    Inventors: Stuart Builder, Roger Hart, Philip Lester, David Reifsnyder
  • Refolding of polypeptides
    Patent number: 5756672
    Abstract: A composition is provided comprising about 0.1 to 15 mg/mL of a polypeptide in a buffer having a pH of about 7-12 comprising about 5-40% (v/v) of an alcoholic or polar aprotic solvent, about 0.2 to 3M of an alkaline earth, alkali metal, or ammonium salt, about 0.1 to 9M of a chaotropic agent, and about 0.01 to 15 .mu.M of a copper or manganese salt. The buffer is suitably used in a method for refolding improperly folded polypeptides.
    Type: Grant
    Filed: June 6, 1995
    Date of Patent: May 26, 1998
    Assignee: Genentech, Inc.
    Inventors: Stuart Builder, Roger Hart, Philip Lester, David Reifsnyder
  • Aqueous multiple-phase isolation of polypeptide
    Patent number: 5723310
    Abstract: A method is described for isolating an exogenous polypeptide in a non-native conformation from cells, such as an aqueous fermentation broth, in which it is prepared comprising contacting the polypeptide with a chaotropic agent and preferably a reducing agent and with phase-forming species to form multiple aqueous phases, with one of the phases being enriched in the polypeptide and depleted in the biomass solids and nucleic acids originating from the cells. Preferably, the method results in two aqueous phases, with the upper phase being enriched in the polypeptide.
    Type: Grant
    Filed: February 7, 1995
    Date of Patent: March 3, 1998
    Assignee: Genentech, Inc.
    Inventors: Stuart Builder, Roger Hart, Philip Lester, John Ogez, David Reifsnyder
  • Aqueous multiple-phase isolation of polypeptide
    Patent number: 5695958
    Abstract: A method is described for isolating an exogenous polypeptide in a non-native conformation from cells, such as an aqueous fermentation broth, in which it is prepared comprising contacting the polypeptide with a chaotropic agent and preferably a reducing agent and with phase-forming species to form multiple aqueous phases, with one of the phases being enriched in the polypeptide and depleted in the biomass solids and nucleic acids originating from the cells. Preferably, the method results in two aqueous phases, with the upper phase being enriched in the polypeptide.
    Type: Grant
    Filed: May 17, 1995
    Date of Patent: December 9, 1997
    Assignee: Genentech, Inc.
    Inventors: Stuart Builder, Roger Hart, Philip Lester, John Ogez, David Reifsnyder
  • Refolding of misfolded insulin-like growth factor-I
    Patent number: 5663304
    Abstract: A composition is provided comprising about 0.1 to 15 mg/mL of a polypeptide in a buffer having a pH of about 7-12 comprising about 5-40% (v/v) of an alcoholic or polar aprotic solvent, about 0.2 to 3M of an alkaline earth, alkali metal, or ammonium salt, about 0.1 to 9M of a chaotropic agent, and about 0.01 to 15 .mu.M of a copper or manganese salt. The buffer is suitably used in a method for refolding improperly folded polypeptides.
    Type: Grant
    Filed: August 20, 1993
    Date of Patent: September 2, 1997
    Assignee: Genentech, Inc.
    Inventors: Stuart Builder, Roger Hart, Philip Lester, David Reifsnyder
  • Aqueous multiple-phase isolation of polypeptide
    Patent number: 5407810
    Abstract: A method is described for isolating an exogenous polypeptide in a non-native conformation from cells, such as an aqueous fermentation broth, in which it is prepared comprising contacting the polypeptide with a chaotropic agent and preferably a reducing agent and with phase-forming species to form multiple aqueous phases, with one of the phases being enriched in the polypeptide and depleted in the biomass solids and nucleic acids originating from the cells. Preferably, the method results in two aqueous phases, with the upper phase being enriched in the polypeptide.
    Type: Grant
    Filed: August 20, 1993
    Date of Patent: April 18, 1995
    Assignee: Genentech, Inc.
    Inventors: Stuart Builder, Roger Hart, Philip Lester, John Ogez, David Reifsnyder