Patents by Inventor Stuart E. Builder

Stuart E. Builder has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method for purifying polypeptides
    Patent number: 5451660
    Abstract: A process is provided for selectively separating a polypeptide of interest from components of differing hydrophobicity in a mixture comprising the steps of:(a) passing the mixture through underivatized silica particles such that the polypeptide adheres to the silica particles;(b) washing the silica particles to remove impurities; and(c) eluting the polypeptide from the silica particles with a buffer comprising an alcoholic or polar aprotic solvent and an alkaline earth, an alkali metal, or an inorganic ammonium salt.
    Type: Grant
    Filed: December 13, 1993
    Date of Patent: September 19, 1995
    Assignee: Genentech, Inc.
    Inventors: Stuart E. Builder, Charles V. Olson, David Reifsnyder
  • Purification of insulin-like growth factor
    Patent number: 5446024
    Abstract: A process for purifying IGF-I from variants of IGF-I is provided. In this process a mixture containing IGF-I and its variants is loaded onto a reversed-phase liquid chromatography column and the IGF-I is eluted from the column with a buffer at a pH of about 6-8. The buffer contains an alcoholic or polar aprotic solvent at a concentration of about 20-30% (v/v). This process can be preceded by a hydrophobic-interaction chromatography step.
    Type: Grant
    Filed: December 17, 1993
    Date of Patent: August 29, 1995
    Assignee: Genentech, Inc.
    Inventors: Stuart E. Builder, John R. Ogez, Charles V. Olson, David Reifsnyder
  • Method of purifying recombinant proteins from corresponding host cell proteins
    Patent number: 5411864
    Abstract: A method for purifying a recombinant protein, e.g. human tissue plasminogen activator (t-PA) from a corresponding endogenous protein of the host cell, e.g. Chinese hamster t-PA, is disclosed. The method comprises contacting a fluid containing the recombinant protein with antibodies specifically binding the corresponding endogenous protein. Monoclonal antibodies directed against and an assay for Chinese hamster t-PA are also disclosed.
    Type: Grant
    Filed: November 5, 1987
    Date of Patent: May 2, 1995
    Assignee: Genentech, Inc.
    Inventors: Vincent R. Anicetti, Stuart E. Builder, Billie J. Marks, John R. Ogez, Eric J. Patzer, David A. Vetterlein
  • Process for producing biologically active plasminogen activator in recombinant CHO cells using suspension culture and removing detrimental components from medium
    Patent number: 5053334
    Abstract: A method of producing biologically active human tissue plasminogen activator in suspension culture is provided wherein recombinant Chinese hamster ovary cells are cultured and certain components detrimental to recovery and biological activity are removed as a cell-free filtrate by cross-flow filtration. The human tissue plasminogen activator is recovered from the culture medium.
    Type: Grant
    Filed: May 9, 1990
    Date of Patent: October 1, 1991
    Assignee: Genentech, Inc.
    Inventors: William R. Arathoon, Stuart E. Builder, Anthony S. Lubiniecki, Robert D. van Reis
  • Stabilized human tissue plasminogen activator compositions
    Patent number: 5034225
    Abstract: Disclosed are novel, stable pharmaceutically acceptable compositions containing human tissue plasminogen activator, featuring, for example, an argininium ion containing buffer as a component. Also disclosed are associated means and methods for preparing and using such compositions in various forms.
    Type: Grant
    Filed: March 12, 1990
    Date of Patent: July 23, 1991
    Assignee: Genentech Inc.
    Inventors: William F. Bennett, Stuart E. Builder, Larry A. Gatlin
  • Stabilized human tissue plasminogen activator compositions
    Patent number: 4908205
    Abstract: Disclosed are novel, stable pharmaceutically acceptable compositions containing human tissue plasminogen activator, featuring, for example, an argininium ion containing buffer as a component. Also disclosed are associated means and methods for preparing and using such compositions in various forms.
    Type: Grant
    Filed: March 28, 1988
    Date of Patent: March 13, 1990
    Assignee: Genentech, Inc.
    Inventors: William F. Bennett, Stuart E. Builder, Larry A. Gatlin
  • Stabilized human tissue plasminogen activator compositions
    Patent number: 4777043
    Abstract: Disclosed are novel, stable pharmaceutically acceptable compositions containing human tissue plasminogen activator, featuring, for example, an argininum ion containing buffer as a component. Also disclosed are associated means and methods for preparing and using such compositions in various forms.
    Type: Grant
    Filed: December 17, 1985
    Date of Patent: October 11, 1988
    Assignee: Genentech, Inc.
    Inventors: William F. Bennett, Stuart E. Builder, Larry A. Gatlin
  • Purification and activity assurance of precipitated heterologous proteins
    Patent number: 4620948
    Abstract: A process for isolating and purifying refractile proteins from host cell culture including treating the host cell culture with a buffered solution to solubilize host protein, leaving refractile protein insoluble, treating the insoluble fraction with a strongly denaturing solution containing a reducing agent to solubilize the refractile protein, and renaturing the refractile protein.
    Type: Grant
    Filed: June 1, 1984
    Date of Patent: November 4, 1986
    Assignee: Genentech, Inc.
    Inventors: Stuart E. Builder, John R. Ogez
  • Purification and activity assurance of precipitated heterologous proteins
    Patent number: 4511502
    Abstract: A process for purifying heterologous proteins from higher molecular weight components, including dissolving the heterologous protein in a strong denaturing solution and removing the higher molecular weight components using a molecular sieve or high speed centrifugation.
    Type: Grant
    Filed: June 1, 1984
    Date of Patent: April 16, 1985
    Assignee: Genentech, Inc.
    Inventors: Stuart E. Builder, John R. Ogez