Abstract: Methods and kits for trapping and barcoding discrete biological units in a hydrogel are disclosed. The kits provide barcode units possessing unique identifiers and a moiety for binding biological units. Also included is a hydrogel solution or monomers for preparing a hydrogel solution, and reagents for molecular biology assays. In certain versions, the barcode units are beads that may be pre-bound to a support. The platform facilitates forming complexes between biological units—such as cells, nuclei, or DNA fragments—and the barcode units, then embedding these complexes within a polymerized hydrogel matrix. This enables analyzing gene expression, genotype, haplotype, or the epigenome by allowing reagent diffusion while keeping the biological units and their unique barcodes spatially associated.

Abstract: Disclosed are methods for trapping and barcoding discrete biological units in a hydrogel. Also disclosed are methods for analyzing gene expression, genotype, haplotype or epigenome in discrete biological units, as well as kits for implementing the methods of the present disclosure.

Abstract: Disclosed are methods for trapping and barcoding discrete biological units in a hydrogel. Also disclosed are methods for analyzing gene expression, genotype, haplotype or epigenome in discrete biological units, as well as kits for implementing the methods of the present disclosure.