Abstract: The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetase that can incorporate the unnatural amino acid phenylselenocysteine into proteins produced in eubacterial host cells such as E. coli. The invention provides, for example but not limited to, novel orthogonal aminoacyl-tRNA synthetases, polynucleotides encoding the novel sythetases molecules, methods for identifying and making the novel synthetases, methods for producing containing the unnatural amino acid phenylselenocysteine and translation systems. The invention further provides methods for producing modified proteins (e.g., lapidated proteins) through targeted modification of the phenylselenocysteine residue in a protein.

Abstract: Methods, devices and compositions to preserve plants, such as cut flowers. The methods include, e.g., sealing cut stems in antimicrobial preservative media, sealing the leaves in a substantially unvented compartment and sealing the flower blossoms in a less humid vented environment. Methods include, e.g., sealing cut flower ends in a preservative liquid, sealing the majority of the stem length and leaves in a stem compartment and holding the blossoms and at least a portion of the leaves in a relatively open blossom compartment. The devices include, e.g., packaging materials and preservative to vitalize and protect uprooted plants and/or cut flowers. The compositions of the invention provide, e.g., packaging systems and preservative media which inhibit microbe growth and vitalize cut plants.

Abstract: The present invention provides, e.g., methods, devices and compositions to preserve plants, such as cut flowers. The methods include, e.g., sealing cut stems in antimicrobial preservative media, sealing the leaves in a substantially unvented compartment and sealing the flower blossoms in a less humid vented environment. Methods include, e.g., sealing cut flower ends in a preservative liquid, sealing the majority of the stem length and leaves in a stem compartment and holding the blossoms and at least a portion of the leaves in a relatively open blossom compartment. The devices include, e.g., packaging materials and preservative to vitalize and protect uprooted plants and/or cut flowers. The compositions of the invention provide, e.g., packaging systems and preservative media which inhibit microbe growth and vitalize cut plants.