Abstract: A primer composition, a kit and a method for detecting polymorphic STR markers based on capillary electrophoresis and applications thereof are provided, which are used to simultaneously amplify 13 STR loci on cattle genome. The primer composition includes one or more pairs of primers with sequences as shown in SEQ ID NO: 1˜26. Developmental validation indicated that the kit is of high sensitivity, fine specificity, strong stability and high accuracy. This provides an effective tool for species identification, individual identification and parental analysis of cattle samples in civil disputes and illegal crimes.

Abstract: A primer composition, a kit and a method for detecting microhaplotype loci based on next generation sequencing technology and applications thereof are provided, relating to the technical field of forensic medicine, which are used to amplify 163 microhaplotype loci on human genome. The primer composition includes one or more pairs of primers with sequences as shown in SEQ ID NO: 1˜326. The primer composition involves 163 microhaplotype loci covering 22 autosomes, which can provide more new genetic information in Asian population than the system constructed in the past. In addition, compared with the next generation sequencing kit of STR loci, the kit has better mixture detection capability. Moreover, the microhaplotype genetic markers have high ancestry information content and can distinguish populations in Africa, Europe, South Asia, and East Asia. Therefore, the microhaplotype genetic markers can also be used for ancestry inference in addition to individual identification and parentage testing.

Abstract: A caulking gun includes a push disk, a push rod, an adhesive cartridge compartment, a trigger and a gun body; the trigger is hingedly coupled to the middle part of the gun body; the rear end of the push rod is outside the gun body and provided with a braking device; the front end is in the adhesive cartridge compartment through the gun body and provided with a push disk; a push piece for driving the push rod is mounted in the gun body; the front end of the push piece is provided with a return spring; the trigger is provided with a pin position switching device for adjusting the propulsion ratio by adjusting the contact position of the push piece; adjustment of different propulsion ratios enables the caulking gun to be multifunctional to meet the construction requirements of different adhesives.

Abstract: A caulking gun includes a push disk, a push rod, an adhesive cartridge compartment, a trigger and a gun body; the trigger is hingedly coupled to the middle part of the gun body; the rear end of the push rod is outside the gun body and provided with a braking device; the front end is in the adhesive cartridge compartment through the gun body and provided with a push disk; a push piece for driving the push rod is mounted in the gun body; the front end of the push piece is provided with a return spring; the trigger is provided with a pin position switching device for adjusting the propulsion ratio by adjusting the contact position of the push piece; adjustment of different propulsion ratios enables the caulking gun to be multifunctional to meet the construction requirements of different adhesives.