Abstract: The present invention relates to a recombinant expression vector, pSSA-K or pSSA-H, containing oxidative stress inducible promoter, such as sweetpotato peroxidase anionic 2 (SWEPA2), a nucleotide encoding superoxide dismutase (SOD) and a nucleotide sequence encoding ascorbate peroxidase (APX) linked to the promoter, antibiotics resistant gene, tobacco etch virus leader sequence, a polynucleotide encloding a transit peptide for chloroplast targeted expression, CaMV 35S transcription terminator, and T-DNA boarder sequence, and a method for preparing multiple stress tolerant transgenic plants using the recombinant expression vector.

Abstract: The present invention relates to a recombinant expression vector for production of multiple stress tolerant plants, which is prepared by attaching multiple stress tolerant genes to oxidative stress inducible promoter, multiple stress tolerant plants transformed with the expression vector and a preparation method for the plants, more precisely, a recombinant expression vector for production of multiple stress tolerant plants that is prepared by combining oxidative stress inducible peroxidase promoter (SWPA2) originated from sweetpotato with multiple stress tolerant genes (SOD (superoxide dismutase) and APX (ascobate peroxidase)) to express the multiple stress tolerant genes in chloroplasts, multiple stress tolerant plants transformed with the expression vector above and a preparation method for the plants.

Abstract: The present invention relates to a multiple stress inducible promoter, more particularly to a multiple stress inducible peroxidase promoter derived by Ipomoea batatas, a expression vector for production of transgenic plants with enhanced tolerance to multiple stress containing the promoter, a transgenic plant cell and plants transduced by the expression vector, and a method for generating the transgenic plants. Promoter of present invention will be biotechnologically useful for development of industrial transgenic plants with enhanced toleransce to multiple stress and plant cell lines to produce useful proteins.

Abstract: The present invention relates to a method for producing target protein in plant cell culture, more particularly to the method for producing target protein comprising the steps of i) constructing vector containing SWPA2 promoter and gene for the target protein, ii) introducing said vector into Agrobacterium, iii) transforming said Agrobacterium into plant cell, and iv) mass-producing target protein using said transformed plant cells. The method of the present invention can mass-produce target proteins such as human lactoferrin. Moreover, the transformed plant can also be used to make health foods when medicinal plant is used as a host cell.

Abstract: The present invention relates to a method for high throughput screening of plant growth regulator, more particularly to the method comprising; 1) culturing phytomixotrophic cells and candidates of plant growth regulator which were added in a microwell plate, 2) treating 2,3,5-triphenyltetrazolium chloride thereto, 3) reacting thereof by adding ethanol after removing solutions from microwells, 4) transferring the reacting solution into the new microwell plate, and 5) measuring optical density with a high throughput screening reader. Since the method of the present invention can rapidly and conveniently screen many samples and can also evaluate in vivo activities of plant growth regulators, it can effectively be used as a screening method for plant growth inhibitors and activators.

Abstract: The present invention relates to a method for producing target protein in plant cell culture, more particularly to the method for producing target protein comprising the steps of i) constructing vector containing SWPA2 promoter and gene for the target protein, ii) introducing said vector into Agrobacterium, iii) transforming said Agrobacterium into plant cell, and iv) mass-producing target protein using said transformed plant cells. The method of the present invention can mass-produce target proteins such as human lactoferrin. Moreover, the transformed plant can also be used to make health foods when medicinal plant is used as a host cell.

Abstract: The present invention relates to a stress inducible promoter. Particularly, it relates to an Ipomoea batatas peroxidase isozyme gene promoter which is strongly induced under environmental stresses in cultured cells and whole plants. The promoter of the present invention can effectively be used to develop stress-tolerant plants which have resistance to environmental stresses, and to develop transformed organisms which can produce useful materials on a large scale.

Abstract: The invention relates to a transgenic plant that produces high levels of superoxide dismutase (SOD) and to a method for producing the transgenic plant. The hypocotyl section of seedlings is co-cultured with Agrobacterium transformant and regenerated by adventitious shoot induction and by root induction, where the Agrobacterium transformant contains an expression vector that comprises the promoter of a fruit-dominant ascorbate oxidase gene, an SOD gene isolated from cassava, and an herbicide-resistant bar gene. The present invention also relates to a method for inducing adventitious shoot from hypocotyl sections in plant tissue culture, thus providing a method for the efficient production of transgenic plants maintaining higher SOD activity in fruits. Therefore, the SOD transgenic cucumber of the present invention can be used for cosmetics, additives in functional foods, and medicines as well as having tolerance to herbicides and environmental stresses.