Abstract: A qualitative and quantitative hydrolase detection method includes providing a caged coelenterazine molecule having a detection molecule bonded between a first bonding site or a second bonding site of a coelenterazine molecule and a steric caging group; activating an uncaged coelenterazine molecule by cleaving the detection molecule in the caged coelenterazine molecule with a hydrolase enzyme; determining a light emission from a coelenterazine-activated bioluminescent enzyme reacting with the uncaged coelenterazine molecule activated in the presence of the hydrolase enzyme; and correlating the light emission for quantitatively and qualitatively determining the presence of the hydrolase enzyme.

Abstract: An enzyme detection method includes forming a caged substrate; releasing an uncaged substrate by cleaving a caging molecule from the caged substrate; and emitting a light emission from a Bioluminescence Resonance Energy Transfer luminescent nanocrystal conjugate reacting with the uncaged substrate.

Abstract: A modified solid-support for use as a supported reagent for use in reduction reactions, including reductive amination comprising a solid-support having an tetrasubstituted ammonium cation species ionically bonded to a borohydride anion species extending therefrom having the general formula: wherein P is a solid-support, S is a spacer group selected from the group consisting of alkylene, {including —(CH2)n—}, alkyleneoxy {including -0(CH2)n—}, alkylenethio {including —S(CH2)n—} and alkylenecarboxy, {including —O(O)C—(CH2)n—}, n=2-16. R1, R2, R3 and R are each independently selected from the group consisting of alkyl, cycloalkyl, haloalkyl and aryl. Y equals 1, 2 or 3. H is hydrogen or deuterium.