Abstract: A method for standardizing system response of spectrophotometer which corrects the difference between spectrophotometric system responses generated due to the difference in response characteristics of a light source, wavelength selector, and sensor, and involves obtaining the difference spectrum between a master unit and a slave unit relative to a standard material and standardizing the response characteristic of the slave unit to the response characteristic of the master unit by subtracting the difference spectrum from the spectrum of each sample to be measured by the slave unit. With an instrument which is constituted of a light source, wavelength selector, and sensor and to which a NIR spectroscopy is applied, a shift of an absorbance value at each wavelength of the slave unit from the absorbance value of the master unit is similarly generated in each sample to be measured.

Abstract: A method of analyzing blood using a near infrared apparatus, in which monochromatic near infrared light in a wavelength range of 700 nm-1100 nm from the slit of the near infrared apparatus is applied to a ceramic plate through an optical fiber to measure a transmitted light intensity of the ceramic plate, which is a reference material for spectrum measurement. Next, in place of the ceramic plate, a blood collection tube containing a blood sample which has been stabilized at a predetermined temperature by a water bath has the near infrared light applied thereto. A so-called near infrared absorption spectrum in which absorbance has been plotted against wavelengths is obtained and information about object characteristics of the blood is extracted from the spectrum data using a calibration equation predetermined using the near infrared apparatus in relation to blood specimens with different, known object characteristics.

Abstract: First, monochromatic near infrared light in a wavelength range of 700 nm-1100 nm from the slit of the near infrared apparatus 1 is applied to a reference ceramic plate through the optical fiber 7 to measure a transmitted light intensity of the ceramic plate which is a reference material for spectrum measurement. Next, in place of the ceramic plate, the test tube 4 containing a liquid sample of which the temperature has been adjusted at a predetermined temperature by a water bath and the like is inserted into the housing portion 5. The transmitted light intensity of the liquid sample is then measured using the same procedure as above. A so-called near infrared absorption spectrum in which absorbance has been plotted against wavelengths is displayed on the screen of the computer 2. Information about each object characteristic is extracted from the spectrum data using a calibration equation.

Abstract: A method of analyzing blood using a near infrared apparatus, in which monochromatic near infrared light in a wavelength range of 700 nm-1100 nm from the slit of the near infrared apparatus is applied to a ceramic plate through an optical fiber to measure a transmitted light intensity of the ceramic plate, which is a reference material for spectrum measurement. Next, in place of the ceramic plate, a blood collection tube containing a blood sample which has been stabilized at a predetermined temperature by a water bath has the near infrared light applied thereto. A so-called near infrared absorption spectrum in which absorbance has been plotted against wavelengths is obtained and information about object characteristics of the blood is extracted from the spectrum data using a calibration equation predetermined using the near infrared apparatus in relation to blood specimens with different, known object characteristics.