Patents by Inventor Sumio Kitahata
Sumio Kitahata has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 5763598Abstract: Disclosed are novel branched cyclodextrins having any of an N-acetylglucosamine group, a glucosaminyl group, an N-acetylgalactosaminyl group and a galactosaminyl group as bonded to the hydroxyl group of the cyclodextrin ring by .beta.-bonding therebetween, and their salts. The novel branched cyclodextrins are produced by reacting a solution containing a cyclodextrin and an N-acetylglucosamine or N-acetylgalactosamine with an N-acetylhexosaminidase, and optionally deacetylating the resulting product.Type: GrantFiled: June 16, 1997Date of Patent: June 9, 1998Assignee: Ensuiko Sugar Refining Co., Ltd.Inventors: Kenichi Hamayasu, Tetsuya Ito, Koki Fujita, Kozo Hara, Kyoko Koizumi, Toshiko Tanimoto, Hirofumi Nakano, Sumio Kitahata
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Patent number: 5623071Abstract: A cyclodextrin having a galactosyl group which is bonded to a 6-hydroxyl group of a glucosyl group of the cyclodextrin via an .alpha.-bond or a .beta.-bond; a cyclodextrin having a mannosyl group which is bonded to a 6-hydroxyl group of the cyclodextrin via an .alpha.-bond.Type: GrantFiled: June 1, 1995Date of Patent: April 22, 1997Assignees: Ensuiko Sugar Refining Co., Ltd., Sumio KitahataInventors: Sumio Kitahata, Koji Hara, Koki Fujita, Nobuhiro Kuwahara, Kyoko Koizumi
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Patent number: 5622844Abstract: A method of producing a mannosyl-cyclodextrin having a mannosyl group bonded to the hydroxyl group of the glucosyl group of a cyclodextrin via an alpha-bond. The method includes treating a liquid containing a cyclodextrin and an alpha-mannosyl compound with an alpha-mannosyl transfer agent.Type: GrantFiled: June 1, 1995Date of Patent: April 22, 1997Assignees: Ensuiko Sugar Refining Co., Ltd., Sumio KitahataInventors: Sumio Kitahata, Koji Hara, Koki Fujita, Nobuhiro Kuwahara, Kyoko Koizumi
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Patent number: 5593869Abstract: Novel trehalase having the properties that it hydrolyzes .alpha.,.alpha.'-trehalose, 2,2'-dideoxy-.alpha.,.alpha.'-trehalose and 2-deoxy-.alpha.,.alpha.'-trehalose into the respective constituting sugars while it does not act on neotrehalose, lactose, maltose, celobiose and sucrose; its optimum pH is 5 to 6; its optimum temperature is 65.degree. C.; it is stable against heating up to 65.degree. C.; its molecular weight as measured by a gel filtration is 400,000 to 500,000 while the molecular weight of the subunit as measured by a sodium dodecylsulfate--polyacrylamide gel electrophoresis is 180,000 to 250,000; its isoelectric point as measured by an isoelectricfocusing is 2.7; and it is a glycoprotein.Type: GrantFiled: June 7, 1995Date of Patent: January 14, 1997Assignees: San-Ei Gen F.F.I, OsakaInventors: Sumio Kitahata, Hirofumi Nakano, Tsutomu Washino, Masamitsu Moriwaki
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Patent number: 5529927Abstract: Novel trehalase having the properties that it hydrolyzes .alpha.,.alpha.'-trehalose, 2,2'-dideoxy-.alpha.,.alpha.'-trehalose and 2-deoxy-.alpha.,.alpha.'-trehalose into the respective constituting sugars while it does not act on neotrehalose, lactose, maltose, celobiose and sucrose; its optimum pH is 5 to 6; its optimum temperature is 65.degree. C.; it is stable against heating up to 65.degree. C.; its molecular weight as measured by a gel filtration is 400,000 to 500,000 while the molecular weight of the subunit as measured by a sodium dodecylsulfate--polyacrylamide gel electrophoresis is 180,000 to 250,000; its isoelectric point as measured by an isoelectricfocusing is 2.7; and it is a glycoprotein.Type: GrantFiled: April 22, 1994Date of Patent: June 25, 1996Assignee: San-Ei Gen F.F.I., Inc.Inventors: Sumio Kitahata, Hirofumi Nakano, Tsutomu Washino, Masamitsu Moriwaki
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Patent number: 5523218Abstract: Disclosed are (1) a novel galactosyl-cyclodextrin having a galactosyl group as bonded to the hydroxyl group of the glucosyl group of a cyclodextrin via an .alpha.-bond and a method of producing said galactosyl-cyclodextrin; (2) a novel galactosyl-cyclodextrin having a galactosyl group as bonded to the hydroxyl group of the glucosyl group of a cyclodextrin via a .beta.-bond and a method of producing said galactosyl-cyclodextrin; and (3) a novel mannosyl-cyclodextrin having a mannosyl group as bonded to the hydroxyl group of the glucosyl group of a cyclodextrin via an .alpha.-bond and a method of producing said mannosyl-cyclodextrin. The new branched cyclodextrins are expected to be widely useful in various fields of drug industry, food industry and cosmetic industry.Type: GrantFiled: December 30, 1994Date of Patent: June 4, 1996Assignees: Ensuiko Sugar Refining Co., Ltd., Sumio KitahataInventors: Sumio Kitahata, Koji Hara, Koki Fujita, Nobuhiro Kuwahara, Kyoko Koizumi
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Patent number: 5463039Abstract: A method of preparing a hetero-branched cyclodextrin comprising a glucosyl or a maltosyl group side chain and a mannosyl group .alpha.-bonded to a hydroxyl group of the glucosyl group or the maltosyl group of the side chain, the method involving reacting a liquid containing a branched cyclodextrin having a glucosyl or maltosyl group as the side chain and an .alpha.-mannosyl compound with an .alpha.-mannosyl transfer enzyme.Type: GrantFiled: June 21, 1994Date of Patent: October 31, 1995Assignees: Ensuiko Sugar Refining Co., Ltd., Osaka-shi, Kyoko KoizumiInventors: Koji Hara, Koki Fujita, Nobuhiro Kuwahara, Sumio Kitahata, Kyoko Koizumi
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Reagent for Determining .alpha.-amylase activity and method for determining .alpha.-amylase activity
Patent number: 5393660Abstract: A reagent for determining a-amylase activity, comprising a maltooligosaccharide derivative of the following formula ##STR1## (wherein either one of R.sub.1 and R.sub.2 is .beta.-galactopyranosil and the other is hydrogen, R.sub.3 is a group bonded to the reducing terminal glucose via a bond cleavable by .alpha.-amylase, which becomes a measurable substance upon cleavage of the bond, and n is an integer of 0-2), which does not comprise adjuvant enzymes; and a method for determining .alpha.-amylase activity which comprises use of the reagent. The reagent of the present invention does not require use of any adjuvant enzyme and is stable since the substrate is not exposed to the decomposition by an adjuvant enzyme. The substrate used in the present invention has high affinity for .alpha.-amylase. Thus, the reagent and the determination method of the present invention make it possible to determine .alpha.-amylase activity with high sensitivity.Type: GrantFiled: November 4, 1993Date of Patent: February 28, 1995Assignees: Toyo Boseki Kabushiki Kaisha, Ensuiko Sugar Refining Co., Ltd.Inventors: Sumio Kitahata, Nobuhiro Kuwahara, Koki Fujita, Koji Hara, Keiichi Majima, Shin-ichi Teshima, Yuzo Hayashi -
Patent number: 5366879Abstract: Disclosed is a method of producing a branched cyclodextrin where a liquid containing a cyclodextrin and a branched oligosaccharide is treated with a debranching enzyme. By the method, a branched cyclodextrin having a maltosyl or maltotriosyl group as bonded to the glucose of a CD molecule via an .alpha.-1,6 bond is obtained efficiently.Type: GrantFiled: December 17, 1992Date of Patent: November 22, 1994Assignees: Ensuiko Sugar Refining Co., Ltd., Sumio Kitahata, Hirofumi NakanoInventors: Sumio Kitahata, Koji Hara, Koki Fujita, Nobuhiro Kuwahara, Hirofumi Nakano
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Patent number: 5356884Abstract: A hetero-branched cyclodextrin (CD) having a glucosyl or maltosyl group as the side chain, in which a mannosyl group is .alpha.-bonded to the hydroxyl group of the glucosyl group of the side chain; and a method of efficiently preparing the same by reacting a branched cyclodextrin with an .alpha.-mannosidase. The hetero-branched CDs are useful in various fields such as in drugs, foods and cosmetics.Type: GrantFiled: October 8, 1992Date of Patent: October 18, 1994Assignees: Ensuiko Sugar Refining Co., Ltd., Osaka-shi, Kyoko KoizumiInventors: Koji Hara, Koki Fujita, Nobuhiro Kuwahara, Sumio Kitahata, Kyoko Koizumi
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Patent number: 5089401Abstract: An enzymatic method for the preparation of a fructose-containing oligosaccharide, in which a .beta.-fructofuranosidase obtained by culturing Arthrobacter sp. K-1 (FERM BP-3192) as an enzyme is reacted on sucrose, raffinose or stachyose as the donor in the presence of an aldose or ketose as the receptor. The enzyme is characterized by:(1) activity on sucrose for the transglycosidation of a fructosyl group to the receptor in the presence of a monosaccharide, sugar alcohol, alkyl alcohol, glycoside or oligosaccharide;(2) activity for the decomposition of sucrose, elrose, neokestose, xylsucrose, raffinose and stachyose with inactivity on a saccharide selected from the group consisting of 1-kestose, nistose, inulobiose and levan biose;(3) optimum pH of 6.5 to 6.8 at 40.degree. C. with stability at a pH of 5.5 to 10;(4) optimum temperature of 55.degree. C. at a pH of 6.5 exhibiting at least 70% of residual activity at 60.degree. C.Type: GrantFiled: March 20, 1991Date of Patent: February 18, 1992Assignee: Ensuiko Sugar Refining Co., Ltd.Inventors: Koki Fujita, Kozo Hara, Hitoshi Hashimoto, Sumio Kitahata
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Patent number: 4454161Abstract: The present invention relates to a process for the production of branching enzyme, and a method for improving the qualities of food products therewith. According to the present invention, a large amount of branching enzyme with a high specific activity can be easily obtained, and employment of the branching enzyme in food processing remarkably improves the qualities of food products without changing the desirable inherent properties of their amylaceous constituent(s); thus, said food products retain their qualities over a long period of time, prolonging extremely their shelf lives.Type: GrantFiled: January 4, 1982Date of Patent: June 12, 1984Assignee: Kabushiki Kaisha Hayashibara Seibutsu Kagaku KenkyujoInventors: Shigetaka Okada, Sumio Kitahata, Shigeharu Yoshikawa, Toshiyuki Sugimoto, Kaname Sugimoto
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Patent number: 4254227Abstract: The present invention relates to processes for producing syrups or syrup solids containing fructose-terminated oligosaccharides, characterized by subjecting a mixture containing liquefied starch and either fructose or sucrose to the action of immobilized cyclodextrin glucanotransferase E.C. 2.4.1.19.Type: GrantFiled: March 8, 1979Date of Patent: March 3, 1981Assignee: Kabushiki Kaisha Hayashibara Seibutsu Kagaku KenkyujoInventors: Shigetaka Okada, Sumio Kitahata, Shigeharu Yoshikawa, Kentaro Miyake