Abstract: The present disclosure relates to a composition for cell imaging including a probe compound, and a method of cell-material imaging using the same. The composition for cell imaging and the method of cell-material imaging are provided to image the material in the cell by measuring fluorescence generated by reacting the probe and a material in a cell.

Abstract: The present application relates to a method of producing or purifying an FGF21 protein by using the heat-elasticity of FGF21. The method of producing or purifying the FGF21 protein according to an embodiment of the present application utilizes the heat stability and heat-elasticity of the FGF21 protein that is not denatured or precipitated even upon heat treatment and returns to the original structure when re-cooled after heat treatment, resulting in advantages of improving efficiency by simplifying a protein production process.

Abstract: The present disclosure relates to an artificial metabolon formed by selective self-assembly, production and use thereof, specifically, an artificial metabolon formed by de novo, in vivo assembly of multi-step metabolic pathway enzymes without using any external scaffold, production and use thereof.

Abstract: The present disclosure relates to a composition for cell imaging, including a fluorescent probe compound, and a method of cell-material imaging using the same.

Abstract: The present invention relates to novel hydrogenases isolated from novel hyperthermophilic strains belonging to Thermococcus spp., genes encoding the hydrogenases, and methods of producing hydrogen using strains having the genes. According to the hydrogen production methods of the invention, a large amount of hydrogen can be produced merely by culturing the strains in specific culture conditions. Thus, the methods of the invention have advantages in that they are more economic and efficient than existing hydrogen production methods and can produce hydrogen even at high temperature.

Abstract: The present application relates to a strain expressing the FrsA protein, and a method for producing ethanol using the same. The FrsA of the present application has a high PDC enzyme activity for a pyruvate, which is a substrate, and thus can be used in a process for producing ethanol. In addition, an FrsA mutant having improved stability in a host cell can be more effective in producing ethanol due to the increase in stability when the FrsA mutant is overexpressed together with IIAGlc, compared with when using conventional Zymomonas mobilis-derived PDC.

Abstract: The present invention relates to novel hydrogenases isolated from novel hyperthermophilic strains belonging to Thermococcus spp., genes encoding the hydrogenases, and methods of producing hydrogen using strains having the genes. According to the hydrogen production methods of the invention, a large amount of hydrogen can be produced merely by culturing the strains in specific culture conditions. Thus, the methods of the invention have advantages in that they are more economic and efficient than existing hydrogen production methods and can produce hydrogen even at high temperature.

Abstract: The present application relates to a strain expressing the FrsA protein, and a method for producing ethanol using the same. The FrsA of the present application has a high PDC enzyme activity for a pyruvate, which is a substrate, and thus can be used in a process for producing ethanol.

Abstract: The present invention relates to novel hydrogenases isolated from novel hyperthermophilic strains belonging to Thermococcus spp., genes encoding the hydrogenases, and methods of producing hydrogen using strains having the genes. According to the hydrogen production methods of the invention, a large amount of hydrogen can be produced merely by culturing the strains in specific culture conditions. Thus, the methods of the invention have advantages in that they are more economic and efficient than existing hydrogen production methods and can produce hydrogen even at high temperature.

Abstract: The present invention relates to novel hydrogenases isolated from novel hyperthermophilic strains belonging to Thermococcus spp., genes encoding the hydrogenases, and methods of producing hydrogen using strains having the genes. According to the hydrogen production methods of the invention, a large amount of hydrogen can be produced merely by culturing the strains in specific culture conditions. Thus, the methods of the invention have advantages in that they are more economic and efficient than existing hydrogen production methods and can produce hydrogen even at high temperature.

Abstract: The present invention relates to novel hydrogenases isolated from novel hyperthermophilic strains belonging to Thermococcus spp., genes encoding the hydrogenases, and methods of producing hydrogen using strains having the genes. According to the hydrogen production methods of the invention, a large amount of hydrogen can be produced merely by culturing the strains in specific culture conditions. Thus, the methods of the invention have advantages in that they are more economic and efficient than existing hydrogen production methods and can produce hydrogen even at high temperature.

Abstract: The present invention related to a method for crystallizing a CMY-10 being a ?-lactamase with extended-substrate spectrum, a crystal of CMY-10, and a crystal structure of CMY-10. With utilization of three-dimensional structure of CMY-10 protein provided by the present invention, it is possible to develop novel antibiotics or inhibitors that can prevent an emergence of resistance bacteria appeared by plasmidic class C ?-lactamases having extended-substrate specificity.

Abstract: The present invention related to a method for crystallizing a CMY-10 being a ?-lactamase with extended-substrate spectrum, a crystal of CMY-10, and a crystal structure of CMY-10. With utilization of three-dimensional structure of CMY-10 protein provided by the present invention, it is possible to develop novel antibiotics or inhibitors that can prevent an emergence of resistance bacteria appeared by plasmatic class C ?-lactamases having extended-substrate specificity.

Abstract: The present invention relates to novel hydrogenases isolated from novel hyperthermophilic strains belonging to Thermococcus spp., genes encoding the hydrogenases, and methods of producing hydrogen using strains having the genes. According to the hydrogen production methods of the invention, a large amount of hydrogen can be produced merely by culturing the strains in specific culture conditions. Thus, the methods of the invention have advantages in that they are more economic and efficient than existing hydrogen production methods and can produce hydrogen even at high temperature.

Abstract: The present invention related to a method for crystallizing a CMY-10 being a ?-lactamase with extended-substrate spectrum, a crystal of CMY-10, and a crystal structure of CMY-10. With utilization of three-dimensional structure of CMY-10 protein provided by the present invention, it is possible to develop novel antibiotics or inhibitors that can prevent an emergence of resistance bacteria appeared by plasmidic class C ?-lactamases having extended-substrate specificity.

Abstract: The present invention related to a method for crystallizing a CMY-10 being a ?-lactamase with extended-substrate spectrum, a crystal of CMY-10, and a crystal structure of CMY-10. With utilization of three-dimensional structure of CMY-10 protein provided by the present invention, it is possible to develop novel antibiotics or inhibitors that can prevent an emergence of resistance bacteria appeared by plasmidic class C ?-lactamases having extended-substrate specificity.

Abstract: The present invention relates to a method for preparing the crystal of human derived TRAIL protein and TRAIL receptor protein sDR5 complex and the crystal of TRAIL-sDR5 complex prepared by the method. The present invention also TRAIL relates to a three-dimensional structure of TRAIL protein in the form of complex with TRAIL receptor sDR5 as well as TRAIL protein itself, and specific residues existed in the contact central region of AA″ loop. The three-dimensional structure of TRAIL-sDR5 complex and the specific residues in the AA″ loop of the present invention can be effectively used for not only the development of TRAIL protein having the better cytotoxic activity and having the improved receptor biding affinity, which specifically kills viral infected cells or cancer cells but also molecular strategy for regulating the binding specificity between TNF and TNFR family.