Patents by Inventor Sundaram ACHARYA

Sundaram ACHARYA has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Kinetically enhanced engineered FnCas9 and its uses thereof
    Patent number: 11970699
    Abstract: Engineered FnCas9 variants are provided that have an enhanced kinetic activity and a broader PAM recognition. The protein engineering methodology introduced specific mutations that stabilized interaction between Cas9 enzyme and target DNA. The enhanced kinetic activity increases NHEJ-mediated editing, owing to more efficient DSB generation potential than WT FnCas9, and the broadened PAM specificity increases the target range of FnCas9 variants. Thus, the scope and accessibility of CRISPR-Cas9 system targets are widened, along with generating robust and highly specific engineered FnCas9 variants.
    Type: Grant
    Filed: October 25, 2022
    Date of Patent: April 30, 2024
    Assignee: COUNCIL OF SCIENTIFIC & INDUSTRIAL RESEARCH
    Inventors: Debojyoti Chakraborty, Souvik Maiti, Sundaram Acharya
  • KINETICALLY ENHANCED ENGINEERED FnCas9 AND ITS USES THEREOF
    Publication number: 20230146664
    Abstract: Engineered FnCas9 variants are provided that have an enhanced kinetic activity and a broader PAM recognition. The protein engineering methodology introduced specific mutations that stabilized interaction between Cas9 enzyme and target DNA. The enhanced kinetic activity increases NHEJ-mediated editing, owing to more efficient DSB generation potential than WT FnCas9, and the broadened PAM specificity increases the target range of FnCas9 variants. Thus, the scope and accessibility of CRISPR-Cas9 system targets are widened, along with generating robust and highly specific engineered FnCas9 variants.
    Type: Application
    Filed: October 25, 2022
    Publication date: May 11, 2023
    Applicants: COUNCIL OF SCIENTIFIC & INDUSTRIAL RESEARCH, DEPARTMENT OF BIOLOGICAL SCIENCES, GRADUATE SCHOOL OF SCIENCE, THE UNIVERSITY OF TOKYO
    Inventors: Debojyoti CHAKRABORTY, Souvik MAITI, Osamu NUREKI, Hiroshi NISHIMASU, Sundaram ACHARYA, Seichi HIRANO, Hirano Hisato