Patents by Inventor Sundaresh N. Brahmasandra
Sundaresh N. Brahmasandra has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20150118684Abstract: Methods and systems for processing polynucleotides (e.g., DNA) are disclosed. A processing region includes one or more surfaces (e.g., particle surfaces) modified with ligands that retain polynucleotides under a first set of conditions (e.g., temperature and pH) and release the polynucleotides under a second set of conditions (e.g., higher temperature and/or more basic pH). The processing region can be used to, for example, concentrate polynucleotides of a sample and/or separate inhibitors of amplification reactions from the polynucleotides. Microfluidic devices with a processing region are disclosed.Type: ApplicationFiled: October 3, 2014Publication date: April 30, 2015Inventors: Betty Wu, John S. Althaus, Nikhil Phadke, Sundaresh N. Brahmasandra, Kalyan Handique, Aaron Kehrer, Gene Parunak, Cecelia Haley, Ted Springer
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Publication number: 20140377850Abstract: The present invention provides control methods, control systems, and control software for microfluidic devices that operate by moving discrete micro-droplets through a sequence of determined configurations. Such microfluidic devices are preferably constructed in a hierarchical and modular fashion which is reflected in the preferred structure of the provided methods and systems. In particular, the methods are structured into low-level device component control functions, middle-level actuator control functions, and high-level micro-droplet control functions. Advantageously, a microfluidic device may thereby be instructed to perform an intended reaction or analysis by invoking micro-droplet control function that perform intuitive tasks like measuring, mixing, heating, and so forth. The systems are preferably programmable and capable of accommodating microfluidic devices controlled by low voltages and constructed in standardized configurations.Type: ApplicationFiled: June 27, 2014Publication date: December 25, 2014Inventors: Kalyan Handique, Karthik Ganesan, Sundaresh N. Brahmasandra
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Patent number: 8895311Abstract: The present invention provides control methods, control systems, and control software for microfluidic devices that operate by moving discrete micro-droplets through a sequence of determined configurations. Such microfluidic devices are preferably constructed in a hierarchical and modular fashion which is reflected in the preferred structure of the provided methods and systems. In particular, the methods are structured into low-level device component control functions, middle-level actuator control functions, and high-level micro-droplet control functions. Advantageously, a microfluidic device may thereby be instructed to perform an intended reaction or analysis by invoking micro-droplet control function that perform intuitive tasks like measuring, mixing, heating, and so forth. The systems are preferably programmable and capable of accommodating microfluidic devices controlled by low voltages and constructed in standardized configurations.Type: GrantFiled: September 18, 2002Date of Patent: November 25, 2014Assignee: HandyLab, Inc.Inventors: Kalyan Handique, Karthik Ganesan, Sundaresh N. Brahmasandra
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Patent number: 8883490Abstract: The present technology provides for an fluorescent detector that is configured to detect light emitted for a probe characteristic of a polynucleotide. The polynucleotide is undergoing amplification in a microfluidic channel with which the detector is in optical communication. The detector is configured to detect minute quantities of polynucleotide, such as would be contained in a microfluidic volume. The detector can also be multiplexed to permit multiple concurrent measurements on multiple polynucleotides concurrently.Type: GrantFiled: November 14, 2007Date of Patent: November 11, 2014Assignee: HandyLab, Inc.Inventors: Kalyan Handique, Sundaresh N. Brahmasandra
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Publication number: 20140323711Abstract: Methods for processing polynucleotide-containing biological samples, and materials for capturing polynucleotide molecules such as RNA and/or DNA from such samples. The RNA and/or DNA is captured by polyamindoamine (PAMAM (Generation 0)) bound to a surface, such as the surface of magnetic particles. The methods and materials have high efficiency of binding RNA and of DNA, and of release, and thereby permit quantitative determinations.Type: ApplicationFiled: April 25, 2014Publication date: October 30, 2014Applicant: HANDYLAB, INC.Inventors: Sundaresh N. Brahmasandra, Elizabeth Craig
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Patent number: 8852862Abstract: Methods and systems for processing polynucleotides (e.g., DNA) are disclosed. A processing region includes one or more surfaces (e.g., particle surfaces) modified with ligands that retain polynucleotides under a first set of conditions (e.g., temperature and pH) and release the polynucleotides under a second set of conditions (e.g., higher temperature and/or more basic pH). The processing region can be used to, for example, concentrate polynucleotides of a sample and/or separate inhibitors of amplification reactions from the polynucleotides. Microfluidic devices with a processing region are disclosed.Type: GrantFiled: November 16, 2005Date of Patent: October 7, 2014Assignee: HandyLab, Inc.Inventors: Betty Wu, John S. Althaus, Nikhil Phadke, Sundaresh N. Brahmasandra, Kalyan Handique, Aaron Kehrer, Gene Parunak, Cecelia Haley, Ted Springer
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Patent number: 8768517Abstract: The present invention provides control methods, control systems, and control software for microfluidic devices that operate by moving discrete micro-droplets through a sequence of determined configurations. Such microfluidic devices are preferably constructed in a hierarchical and modular fashion which is reflected in the preferred structure of the provided methods and systems. In particular, the methods are structured into low-level device component control functions, middle-level actuator control functions, and high-level micro-droplet control functions. Advantageously, a microfluidic device may thereby be instructed to perform an intended reaction or analysis by invoking micro-droplet control function that perform intuitive tasks like measuring, mixing, heating, and so forth. The systems are preferably programmable and capable of accommodating microfluidic devices controlled by low voltages and constructed in standardized configurations.Type: GrantFiled: June 24, 2013Date of Patent: July 1, 2014Assignee: HandyLab, Inc.Inventors: Kalyan Handique, Karthik Ganesan, Sundaresh N. Brahmasandra
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Patent number: 8710211Abstract: Methods for processing polynucleotide-containing biological samples, and materials for capturing polynucleotide molecules such as RNA and/or DNA from such samples. The RNA and/or DNA is captured by polyamindoamine (PAMAM (Generation 0)) bound to a surface, such as the surface of magnetic particles. The methods and materials have high efficiency of binding RNA and of DNA, and of release, and thereby permit quantitative determinations.Type: GrantFiled: December 3, 2012Date of Patent: April 29, 2014Assignee: Handylab, Inc.Inventors: Sundaresh N. Brahmasandra, Elizabeth Craig
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Publication number: 20140030798Abstract: Methods and systems for processing polynucleotides (e.g., DNA) are disclosed. A processing region includes one or more surfaces (e.g., particle surfaces) modified with ligands that regain polynucleotides under a first set of conditions (e.g., temperature and pH) and release the polynucleotides under a second set of conditions (e.g., higher temperature and/or more basic pH). The processing region can be used to, for example, concentrate polynucleotides of a sample and/or separate inhibitors of amplification reactions from the polynucleotides. Microfluidic devices with a processing region are disclosed.Type: ApplicationFiled: June 24, 2013Publication date: January 30, 2014Applicant: HANDYLAB, INC.Inventors: Betty WU, John S. Althaus, Sundaresh N. Brahmasandra, Kalyan Handique, Nikhil Phadke
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Publication number: 20130280131Abstract: The present invention provides control methods, control systems, and control software for microfluidic devices that operate by moving discrete micro-droplets through a sequence of determined configurations. Such microfluidic devices are preferably constructed in a hierarchical and modular fashion which is reflected in the preferred structure of the provided methods and systems. In particular, the methods are structured into low-level device component control functions, middle-level actuator control functions, and high-level micro-droplet control functions. Advantageously, a microfluidic device may thereby be instructed to perform an intended reaction or analysis by invoking micro-droplet control function that perform intuitive tasks like measuring, mixing, heating, and so forth. The systems are preferably programmable and capable of accommodating microfluidic devices controlled by low voltages and constructed in standardized configurations.Type: ApplicationFiled: June 24, 2013Publication date: October 24, 2013Inventors: KALYAN HANDIQUE, KARTHIK GANESAN, SUNDARESH N. BRAHMASANDRA
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Patent number: 8470586Abstract: Methods and systems for processing polynucleotides (e.g., DNA) are disclosed. A processing region includes one or more surfaces (e.g., particle surfaces) modified with ligands that regain polynucleotides under a first set of conditions (e.g., temperature and pH) and release the polynucleotides under a second set of conditions (e.g., higher temperature and/or more basic pH). The processing region can be used to, for example, concentrate polynucleotides of a sample and/or separate inhibitors of amplification reactions from the polynucleotides. Microfluidic devices with a processing region are disclosed.Type: GrantFiled: May 3, 2005Date of Patent: June 25, 2013Assignee: Handylab, Inc.Inventors: Betty Wu, John S. Althaus, Sundaresh N. Brahmasandra, Kalyan Handique, Nikhil Phadke
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Patent number: 8473104Abstract: The present invention provides control methods, control systems, and control software for microfluidic devices that operate by moving discrete micro-droplets through a sequence of determined configurations. Such microfluidic devices are preferably constructed in a hierarchical and modular fashion which is reflected in the preferred structure of the provided methods and systems. In particular, the methods are structured into low-level device component control functions, middle-level actuator control functions, and high-level micro-droplet control functions. Advantageously, a microfluidic device may thereby be instructed to perform an intended reaction or analysis by invoking micro-droplet control function that perform intuitive tasks like measuring, mixing, heating, and so forth. The systems are preferably programmable and capable of accommodating microfluidic devices controlled by low voltages and constructed in standardized configurations.Type: GrantFiled: July 22, 2011Date of Patent: June 25, 2013Assignee: Handylab, Inc.Inventors: Kalyan Handique, Karthik Ganesan, Sundaresh N. Brahmasandra
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Patent number: 8324372Abstract: Methods for processing polynucleotide-containing biological samples, and materials for capturing polynucleotide molecules such as RNA and/or DNA from such samples. The RNA and/or DNA is captured by polyamidoamine (PAMAM(Generation 0)) bound to a surface, such as the surface of magnetic particles. The methods and materials have high efficiency of binding RNA and of DNA, and of release, and thereby permit quantitative determinations.Type: GrantFiled: July 11, 2008Date of Patent: December 4, 2012Assignee: Handylab, Inc.Inventors: Sundaresh N. Brahmasandra, Elizabeth Craig
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Patent number: 8323900Abstract: The present technology provides for an apparatus for detecting polynucleotides in samples, particularly from biological samples. The technology more particularly relates to microfluidic systems that carry out PCR on nucleotides of interest within microfluidic channels, and detect those nucleotides. The apparatus includes a microfluidic cartridge that is configured to accept a plurality of samples, and which can carry out PCR on each sample individually, or a group of, or all of the plurality of samples simultaneously.Type: GrantFiled: February 25, 2011Date of Patent: December 4, 2012Assignee: HandyLab, Inc.Inventors: Kalyan Handique, Sundaresh N. Brahmasandra, Karthik Ganesan, Jeff Williams
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Patent number: 8105477Abstract: The present invention relates to an electrochemical method for detecting a target polynucleotide. An electrode comprising an electrode surface is provided. The electrode surface includes at least one probe molecule reversibly immobilized with respect to the electrode surface. A first electrochemical signal indicative of an amount of probe molecule immobilized with respect to the electrode surface is obtained. The electrode surface is contacted with a liquid comprising the target polynucleotide. Upon the contacting step, at least some of the probe molecule immobilized with respect to the electrode surface dissociates therefrom. A second electrochemical signal indicative of an amount of probe molecule immobilized with respect to the electrode surface is obtained. The presence of the target polynucleotide is determined at least partially on the basis of the first and second electrochemical signals.Type: GrantFiled: October 22, 2010Date of Patent: January 31, 2012Assignee: Handylab, Inc.Inventors: John S. Althaus, Lee Kyonghoon, Vijay Namasivayam, Sundaresh N. Brahmasandra, Kalyan Handique
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Publication number: 20120022695Abstract: The present invention provides control methods, control systems, and control software for microfluidic devices that operate by moving discrete micro-droplets through a sequence of determined configurations. Such microfluidic devices are preferably constructed in a hierarchical and modular fashion which is reflected in the preferred structure of the provided methods and systems. In particular, the methods are structured into low-level device component control functions, middle-level actuator control functions, and high-level micro-droplet control functions. Advantageously, a microfluidic device may thereby be instructed to perform an intended reaction or analysis by invoking micro-droplet control function that perform intuitive tasks like measuring, mixing, heating, and so forth. The systems are preferably programmable and capable of accommodating microfluidic devices controlled by low voltages and constructed in standardized configurations.Type: ApplicationFiled: July 22, 2011Publication date: January 26, 2012Applicant: HandyLab, Inc.Inventors: Kalyan Handique, Karthik Ganesan, Sundaresh N. Brahmasandra
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Publication number: 20110210257Abstract: The present technology provides for an fluorescent detector that is configured to detect light emitted for a probe characteristic of a polynucleotide. The polynucleotide is undergoing amplification in a microfluidic channel with which the detector is in optical communication. The detector is configured to detect minute quantities of polynucleotide, such as would be contained in a microfluidic volume. The detector can also be multiplexed to permit multiple concurrent measurements on multiple polynucleotides concurrently.Type: ApplicationFiled: November 14, 2007Publication date: September 1, 2011Inventors: Kalyan Handique, Sundaresh N. Brahmasandra
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Publication number: 20110207140Abstract: The present technology provides for an apparatus for detecting polynucleotides in samples, particularly from biological samples. The technology more particularly relates to microfluidic systems that carry out PCR on nucleotides of interest within microfluidic channels, and detect those nucleotides. The apparatus includes a microfluidic cartridge that is configured to accept a plurality of samples, and which can carry out PCR on each sample individually, or a group of, or all of the plurality of samples simultaneously.Type: ApplicationFiled: February 25, 2011Publication date: August 25, 2011Inventors: Kalyan Handique, Sundaresh N. Brahmasandra, Karthik Ganesan, Jeff Williams
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Patent number: 7998708Abstract: The present technology provides for an apparatus for detecting polynucleotides in samples, particularly from biological samples. The technology more particularly relates to microfluidic systems that carry out PCR on nucleotides of interest within microfluidic channels, and detect those nucleotides. The apparatus includes a microfluidic cartridge that is configured to accept a plurality of samples, and which can carry out PCR on each sample individually, or a group of, or all of the plurality of samples simultaneously.Type: GrantFiled: November 14, 2007Date of Patent: August 16, 2011Assignee: HandyLab, Inc.Inventors: Kalyan Handique, Sundaresh N. Brahmasandra, Karthik Ganesan, Jeff Williams
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Patent number: D665095Type: GrantFiled: April 14, 2011Date of Patent: August 7, 2012Assignee: HandyLab, Inc.Inventors: Kerry Wilson, Kalyan Handique, Jeff Williams, Sundaresh N. Brahmasandra