Abstract: The method and system are adapted to brew a matcha beverage into a single-serve pod where the beverage mixture has excess pressure during the brewing process. The beverage mixture includes matcha powder and tencha, where the tencha does not affect the taste of the matcha because the tencha is the precursor to making the matcha by grinding the tencha to make matcha powder. The tencha is coarser than matcha powder, so that the water may flow through the beverage mixture to reduce the pressure. Alternatively, sugar may be incorporated into the beverage mixture to increase the extraction of the matcha. The water flow rate through the pod may be lowered from the beginning to the end of the brewing process, so that the later cycle of the brewing process may have a lower pressure.

Abstract: This application provides methods and compositions related to constructing nucleic acid hydrogels (e.g., RNA hydrogels) having repetitive monomer units, each monomer unit includes one or more G-quadruplex sequences. These G-quadruplex sequences cross-link the nucleic acid concatemer such that it self-assembles into a hydrogel under appropriate conditions. In some embodiments, each monomeric unit of the nucleic acid concatemer comprises a coding sequence for polypeptide of interest; and the nucleic acid hydrogel formed by the nucleic acid concatemer can be used for expressing the polypeptide in high quantities. In some embodiments, at least two RNA concatemers comprising G-quadruplex sequences are produced, one further comprising a spacer and the other further comprising a sequence encoding a polypeptide of interest. These two RNA concatemers are combined and self assembled to form a single, wideband RNA hydrogel.

Abstract: A beverage pod is assembled to allow the upper extension of the filter to separate from the upper rim of the container upon an application of force upon the extension. An energy director may be provided between the extension and the rim such that when a linear vertical high-frequency energy is applied to the energy director, the energy director may melt and releasably join the extension to the rim. At least one of the extension and/or the rim may have an energy director protruding therefrom. Separating the extension from the rim may form a gap between the extension and the rim to allow the beverage formed within the pod to drain therethrough.

Abstract: A system of assembling first and second types of pods includes a first assembly apparatus for packing first type of pods such as low-pressure coffee pods, and a second assembly apparatus for partially packing second types of pods where a second type of filter is packed with a second beverage ingredient without a container. The assembled filter with the second beverage ingredient is passed to the first assembly apparatus where the outer container and the lid may be applied to assemble the second type of pods to brew high-pressure beverages such as espresso. The second type of pods can optionally include a third beverage ingredient separated by a perforated barrier between the second and third beverage ingredients.

Abstract: A redispersible polymer powder composition includes at least one solvent selected from a process oil and an organic solvent, and a vinylacetate-ethylene (VAE)-based redispersible polymer powder pre-swelled by the solvent. The swelling ratio of the vinylacetate-ethylene (VAE)-based redispersible polymer powder exceeds 55%, measured by the equation swelling ratio (%)=[(B?A)/A]×100, where A is the initial mass of the vinylacetate-ethylene (VAE)-based redispersible polymer powder before adding the solvent, and B is the swelled mass of the vinylacetate-ethylene (VAE)-based redispersible polymer powder after adding the solvent and stirring at 300 rpm at room temperature (20° C.) for 30 minutes provided that the mixing ratio of the vinylacetate-ethylene (VAE)-based redispersible polymer powder and the solvent is 1:1 by weight.

Abstract: This application provides methods and compositions related to constructing nucleic acid hydrogels (e.g., RNA hydrogels) having repetitive monomer units, each monomer unit includes one or more G-quadruplex sequences. These G-quadruplex sequences cross-link the nucleic acid concatemer such that it self-assembles into a hydrogel under appropriate conditions. In some embodiments, each monomeric unit of the nucleic acid concatemer comprises a coding sequence for polypeptide of interest; and the nucleic acid hydrogel formed by the nucleic acid concatemer can be used for expressing the polypeptide in high quantities. In some embodiments, at least two RNA concatemers comprising G-quadruplex sequences are produced, one further comprising a spacer and the other further comprising a sequence encoding a polypeptide of interest. These two RNA concatemers are combined and self assembled to form a single, wideband RNA hydrogel.

Abstract: The present invention relates to a nucleic acid template for detection of a target nucleic acid based on a G-quadruplex sequence and a use thereof. A nucleic acid template for detection of a target nucleic acid or a method for detection or diagnosis of a target nucleic acid, using same according to the present invention, allows for simple and rapid detection of a target nucleic acid at room temperature with high specificity through addition and reaction of a reaction enzyme even without a separate PCR machine or a complicated temperature control procedure.

Abstract: A brewing chamber works with a pod to brew a beverage and drains the beverage from the pod without being obstructed by the brewing mechanism to substantially avoid contaminating the beverage and brewing mechanism. The pod is designed to drain the beverage once a gap is formed between an extension of an internal filter that is peelably coupled with a rim of the outer container. The brewing chamber is adapted to hold the pod in a substantially horizontal orientation where a hook slides to separate the extension from the rim to form the gap and the hook is tucked away so that the beverage may drain from the pod unobstructed by the hook.

Abstract: This application provides methods and compositions related to constructing nucleic acid hydrogels (e.g., RNA hydrogels) having repetitive monomer units, each monomer unit includes one or more G-quadruplex sequences. These G-quadruplex sequences cross-link the nucleic acid concatemer such that it self-assembles into a hydrogel under appropriate conditions. In some embodiments, each monomeric unit of the nucleic acid concatemer comprises a coding sequence for polypeptide of interest; and the nucleic acid hydrogel formed by the nucleic acid concatemer can be used for expressing the polypeptide in high quantities. In some embodiments, at least two RNA concatemers comprising G-quadruplex sequences are produced, one further comprising a spacer and the other further comprising a sequence encoding a polypeptide of interest. These two RNA concatemers are combined and self assembled to form a single, wideband RNA hydrogel.

Abstract: An asphalt binder includes an asphalt and an adhesion promoter composition. The adhesion promoter composition includes a vinyl acetate-ethylene (VAE)-based redispersible polymer and a wetting agent. The wetting agent includes at least one selected from the group consisting of: polyethylene glycol phenyl ether, polyethylene glycol higher alkyl, ether dialkyl sulfosuccinate, alkyl amine, alcohol amine, polyethyleneimine, and sorbitan aliphatic ethylene oxide adduct. Further, the wetting agent is included in an amount ranging from 0.5 to 45 percent by weight (wt %) with respect to the total weight of the adhesion promoter composition.

Abstract: The cooling system (100, 300, 400, 500, 700, 750) includes an applicator (101, 501, 701) configured to hold a predetermined amount of solid coolant and fluid coolant (117) to cool a targeted area of the body (162, 570) to crystalize the lipid-rich cells underneath the targeted area to reduce the fat cells. The applicator may include a thermoelectric cooler (TEC, 136, 704, 706) where the hot side is cooled by coolant held within the applicator. The cold side of the TEC may be thermally coupled to a cooling plate (138, 560) configured to cool a targeted area of the skin at a predetermined temperature range for a predetermined period of time. The cooling system may also be used to relieve localized pain at certain area of the body and/or utilized for cryotherapy.

Abstract: A single-serve pod brews a beverage and drains the beverage from the pod without being obstructed by the brewing mechanism to substantially avoid contaminating the beverage and brewing mechanism. The pod has a proximal end and a distal end, where the heated water enters the pod in the proximal end to extract the beverage from the beverage ingredient within the filter and the beverage exits through the distal end of filter where the flow of the beverage is controlled by one or more retainer walls to slow the flow of beverage along the distal end of the pod, and the beverage is redirected to flow towards the proximal end of the pod where it drains between a gap formed during the brewing process.

Abstract: Refillable pod can be reused to brew a beverage with a single-serve brewer. The refillable pod has a funnel at about six O'clock position near the front of the cap to drain the beverage without substantially coming to contact the brewing mechanism. The refillable pod may have a handle which can be used by the user to insert the refillable pod into the brewer so that funnel can be positioned at about six O'clock position within the brewing mechanism.

Abstract: A system of assembling first and second types of pods includes a first assembly apparatus for packing first type of pods such as low-pressure coffee pods, and a second assembly apparatus for partially packing second types of pods where a second type of filter is packed with a second beverage ingredient without a container. The assembled filter with the second beverage ingredient is passed to the first assembly apparatus where the outer container and the lid may be applied to assemble the second type of pods to brew high-pressure beverages such as espresso. The second type of pods can optionally include a third beverage ingredient separated by a perforated barrier between the second and third beverage ingredients.

Abstract: This application provides methods and compositions related to constructing nucleic acid hydrogels (e.g., RNA hydrogels) having repetitive monomer units, each monomer unit includes one or more G-quadruplex sequences. These G-quadruplex sequences cross-link the nucleic acid concatemer such that it self-assembles into a hydrogel under appropriate conditions. In some embodiments, each monomeric unit of the nucleic acid concatemer comprises a coding sequence for polypeptide of interest; and the nucleic acid hydrogel formed by the nucleic acid concatemer can be used for expressing the polypeptide in high quantities. In some embodiments, at least two RNA concatemers comprising G-quadruplex sequences are produced, one further comprising a spacer and the other further comprising a sequence encoding a polypeptide of interest. These two RNA concatemers are combined and self assembled to form a single, wideband RNA hydrogel.

Abstract: A beverage brewing system works with a pod to brew low, medium and high pressure beverages in hot and/or cold format such as coffee and espresso served hot or over ice. The beverage ingredient may be packed within a filter which is enclosed by a container to hermetically seal the filter. The filter has a predetermined number and sized holes to control the pressure within the filter to brew low, medium, and high pressure beverages. The filter is releasably sealed to the container where separating the filter from the container forms a gap to allow the beverage formed within the pod to drain via the gap and directly pour into a mug.

Abstract: A beverage brewing system works with a pod to brew low, medium and high pressure beverages in hot and/or cold format such as coffee and espresso served hot or over ice. The beverage ingredient may be packed within a filter which is enclosed by a container to hermetically seal the filter. The filter has a predetermined number and sized holes to control the pressure within the filter to brew low, medium, and high pressure beverages. The filter is releasably sealed to the container where separating the filter from the container forms a gap to allow the beverage formed within the pod to drain via the gap and directly pour into a mug.

Abstract: According to an aspect, a display device includes a display module including a display panel and a conductive layer, and an enclosure configured to surround the display module, where the enclosure includes a conductive portion. The display device includes an antenna having a structure formed by an air gap disposed between the conductive layer and the conductive portion of the enclosure. The antenna includes an antenna feed located within the air gap. The antenna feed is coupled to the conductive portion of the enclosure and to the conductive layer such that at least a portion of the display module is configured as a radiating element for wireless communication.

Abstract: According to an aspect, a computing device includes a first enclosure, a second enclosure, a hinge coupled to the first enclosure and the second enclosure, and a hinge-based antenna defined by a slot of the hinge and a portion of an air gap disposed between the first enclosure and the second enclosure, where the slot of the hinge is aligned with the portion of the air gap, and the hinge-based antenna includes an antenna feed element disposed within the slot of the hinge and configured to excite the portion of the air gap.

Abstract: An example device may comprise a metallic chassis having a tiered surface comprising an upper face, a connecting face, and a lower face. The connecting face may be arranged between the upper face and the lower face such that the upper face and the connecting face define a raised area. The device may also comprise an antenna cavity formed in the raised area and having an opening in the upper face and the connecting face.