Abstract: The present invention relates to a method and apparatus for analyzing pathology patterns of whole-slide images based on graph deep learning, which may include: a whole-slide image (WSI) compression step of compressing WSI into a superpatch graph; a graph neural networks (GNN) analysis step of embedding node features and context features into the superpatch graph through a GNN model and calculating contributions for each node and edge; a biomarker acquisition step of classifying and grouping the superpatch graph according to the contributions for each node, connecting the classified and grouped superpatch graph in units of groups to generate connected graphs, normalizing and clustering features of the connected graphs, and acquiring environmental graph biomarkers for each group; and a diagnostic information extraction step of extracting and providing diagnostic information on the WSI based on the environmental graph biomarker for each group.

Abstract: The present invention relates to a method and apparatus for analyzing pathology patterns of whole-slide images based on graph deep learning, which may include: a whole-slide image (WSI) compression step of compressing WSI into a superpatch graph; a graph neural networks (GNN) analysis step of embedding node features and context features into the superpatch graph through a GNN model and calculating contributions for each node and edge; a biomarker acquisition step of classifying and grouping the superpatch graph according to the contributions for each node, connecting the classified and grouped superpatch graph in units of groups to generate connected graphs, normalizing and clustering features of the connected graphs, and acquiring environmental graph biomarkers for each group; and a diagnostic information extraction step of extracting and providing diagnostic information on the WSI based on the environmental graph biomarker for each group.

Abstract: Provided are a method for purifying a nucleic acid library, and a kit, the method comprising the steps of: providing a nucleic acid library comprising single-stranded template nucleic acids; obtaining a library of complementary nucleic acids by binding complementary nucleic acid units to each base of the strand of the template nucleic acids; introducing at least one modified nucleic acid unit during the binding process of the nucleic acid units; and selectively selecting a nucleic acid having a desired length from the library of complementary nucleic acids using the modified nucleic acid unit. According to the present invention, the nucleic acid library may be purified regardless of the complexity, sequence or length of the nucleic acid library, and nucleic acids having different lengths may be simultaneously purified. The purification may be carried out through direct experiment or using a next-generation sequencing instrument.

Abstract: The present invention relates to a rapid antibiotic susceptibility test and an identification method capable of rapidly providing information on antibiotics suitable for microorganisms by performing an antibiotic susceptibility test and an identification test in a single process. The present invention provides a rapid antibiotic susceptibility test and an identification method comprising the steps of: (a) transferring a sample taken from a patient to a first reaction space in which a sample separation means is present; (b) separating and concentrating the pathogen in the sample from the patient's sample by capturing them with the separation means within the first reaction space; and (c) performing the identification of the pathogen or an antibiotic susceptibility test.

Abstract: One aspect relates to a nucleic acid amplification method that comprises the step of amplifying nucleic acids by adding a first primer, a second primer and a nucleic acid polymerase to a sample containing a target nucleic acid. According to the nucleic acid amplification method according to one aspect, by performing parallel multiple displacement amplification using the first primer containing the barcode sequence, the increased parallelism and throughput of MDA dramatically reduces the time and cost for NGS library production, the barcode bias phenomenon can be resolved by adjusting the ratio at which the first primer is added, and the quality of amplification can also be improved using a laser-based cell separation method. Therefore, the nucleic acid amplification method can be usefully used for large-scale single-cell whole genome analysis.

Abstract: The present specification discloses a composition, a system, and a method for predicting or diagnosing Alzheimer's disease, comprising a detecting agent of a B cell receptor clonotype specific to an Alzheimer's disease patient, and the composition according to one aspect of the present invention has an excellent effect in predicting or diagnosing Alzheimer's disease by simply using peripheral blood mononuclear cells isolated from a subject, in addition to methods such as MRI and PET scans using radioactive substances, by comprising a detecting agent of a B cell receptor (BCR) clonotype specific to an Alzheimer's disease patient.

Abstract: The polymerase chain reaction (PCR) has limitations, including a lack of efficient search strategies and inefficiencies in securing primer sequence lengths, despite its high sensitivity and specificity. Therefore, the present invention has been devised to address these issues, concerning a composition for the selection of the desired nucleic acid and a method for nucleic acid selection using it. By using the composition and method of the present invention, it becomes possible to hierarchically, efficiently, and selectively detect and amplify subsets of oligonucleotides with high specificity, which is expected to be widely utilized in the overall bio/medical field.

Abstract: The present invention relates to a method and apparatus for analyzing pathology patterns of whole-slide images based on graph deep learning, which may include: a whole-slide image (WSI) compression step of compressing WSI into a superpatch graph; a graph neural networks (GNN) analysis step of embedding node features and context features into the superpatch graph through a GNN model and calculating contributions for each node and edge; a biomarker acquisition step of classifying and grouping the superpatch graph according to the contributions for each node, connecting the classified and grouped superpatch graph in units of groups to generate connected graphs, normalizing and clustering features of the connected graphs, and acquiring environmental graph biomarkers for each group; and a diagnostic information extraction step of extracting and providing diagnostic information on the WSI based on the environmental graph biomarker for each group.

Abstract: Described are stereotypic-naïve SARS-CoV-2 neutralizing antibodies that inhibit that SARS-CoV-2 virus replication. The antibodies comprise variable heavy chain (VH) clonotypes, encoded by either immunoglobulin heavy variable (IGHV)3-53 or IGHV3-66 and immunoglobulin heavy joining (IGHJ)6, and were identified in IgM, IgG3, IgG1, IgA1, IgG2, and IgA2 subtypes, with minimal somatic mutations, and could be paired with diverse light chains, resulting in binding to the SARS-CoV-2 receptor-binding domain (RBD). One of these clonotypes potently inhibited viral replication.

Abstract: Provided is a color encoding method including providing a composition including a liquid medium and magnetic nanoparticles dispersed in the liquid medium; applying a magnetic field to the composition to align the magnetic nanoparticles; and applying a patterned energy source to the composition to solidify the composition, wherein more than one region of the composition are sequentially solidified with varying magnetic field strength to fix a plurality of color codes.

Abstract: Provided are a rapid antimicrobial susceptibility test, based on an analysis of changes in morphology and growth pattern of a microbial cell under different concentrations of various antimicrobial agents, and an automated cell image analysis system therefor. The antimicrobial susceptibility test is rapidly performed based on an analysis of changes in morphology and growth pattern of a microbial cell under different concentrations of various antimicrobial agents, and this makes it possible to obtain highly reliable test results faster by six to seven times than the standard method recommended by Clinical and Laboratory Standards Institute (CLSI).

Abstract: Disclosed is a storage method of DNA digital data, including: encoding a plurality of bit data to a plurality of base sequences including at least one degenerate base; and synthesizing at least two types of bases constituting the at least one degenerate base on a substrate based on a mixing ratio.

Abstract: Provided is a method of isolating biochemical molecules on a microarray substrate, the method including providing a microarray substrate to which clusters of different kinds of biochemical molecules being classified by individual spot units are attached, the individual spots being regularly arranged thereon; obtaining location information of the individual spot in which a desired cluster among clusters of the biochemical molecules locates; locating an extraction tool for applying energy to isolate the desired cluster according to the location information; and isolating the desired cluster from the microarray substrate by applying energy in a contact or non-contact manner using the extraction tool.

Abstract: A testing method is disclosed. The testing method includes: providing a mixture solution of a gelling agent and a microbe to a gelling device; solidifying the mixture solution to form a solid thin film in which the microbe is immobilized; supplying a bioactive agent to the solid thin film and allowing the bioactive agent to diffuse into the solid thin film; and imaging the individual responses of the single microbial cells to the bioactive agent, and determining the minimum inhibitory concentration (MIC) of the bioactive agent based on the analysis of the images to obtain AST results.

Abstract: Provided are encoded polymeric microparticles and a multiplexed bioassay using the encoded polymeric microparticles. Each of the encoded polymeric microparticles includes an encoded polymeric microparticle core and a silica shell surrounding the microparticle core. Further provided is a method for producing encoded polymeric microparticles. The method includes: mixing a photocurable material with a linker having a functional group polymerizable with the photocurable material and an alkoxysilyl group; applying patterned energy to cure the mixture, followed by encoding to obtain encoded polymeric microparticle cores; and treating the encoded polymeric microparticle cores with a silica precursor to form a silica shell on each encoded polymeric microparticle core.

Abstract: The present invention relates to a novel bioactivity testing structure for single cell tracking using a gelling agent and a bioactivity testing system including the testing structure. The present invention also relates to bioactivity testing, drug susceptibility testing, antibiotic screening, and diagnostic methods using the testing structure. The bioactivity testing structure of the present invention enables very rapid and simple drug susceptibility testing of bacteria, particularly Mycobacterium tuberculosis, drug screening, and bacterial diagnosis. Particularly, the use of the testing structure enables DST and diagnosis of bacteria only by pretreatment without the need to concentrate human sputum samples irrespective of inoculum effect, ensuring rapid, accurate, and simple testing compared to conventional tuberculosis diagnosis or DST systems. In addition, the testing structure of the present invention simultaneously enables the diagnosis and drug susceptibility testing of tuberculosis.

Abstract: Disclosed is a storage method of DNA digital data, including: encoding a plurality of bit data to a plurality of base sequences including at least one degenerate base; and synthesizing at least two types of bases constituting the at least one degenerate base on a substrate based on a mixing ratio.

Abstract: Provided are a rapid antimicrobial susceptibility test, based on an analysis of changes in morphology and growth pattern of a microbial cell under different concentrations of various antimicrobial agents, and an automated cell image analysis system therefor. The antimicrobial susceptibility test is rapidly performed based on an analysis of changes in morphology and growth pattern of a microbial cell under different concentrations of various antimicrobial agents, and this makes it possible to obtain highly reliable test results faster by six to seven times than the standard method recommended by Clinical and Laboratory Standards Institute (CLSI).

Abstract: Disclosed is a microcapsule encoded depending on the kind of a target substance included therein. The encoded microcapsule has a hydrophilic liquid core including the target substance and a hydrophobic shell surrounding the liquid core. The encoded microcapsule includes graphical codes introduced on the surface of the shell.

Abstract: Provided is a method of forming a microsphere having a structural color, which includes providing a composition for generating a structural color including a curable material and magnetic nanoparticles dispersed in the curable material, forming an emulsion by adding the composition for generating a structural color to an immiscible solvent, arranging the magnetic nanoparticles located in the emulsion droplet of the curable material in a one-dimensional chain structure by applying a magnetic field to the emulsion, and fixing the chain structure by curing the emulsion droplet.