Abstract: The present invention relates to (1) a method for detecting a specific binding for bio-molecules; (2) a method for detecting a presence of target molecules within a specimen; and (3) a method for measuring a quantity of target molecules existing in a specimen, in which a pair of energy donor and energy acceptor displaying a FRET phenomenon is used to guide a specific binding between a pair of bio-molecules. According to the present invention, the process is conducted rapidly and easily without a label so as to screen a biochemical substance inhibiting a specific binding between a pair of bio-molecules in an ultra-high speed and measure a quantity of the substance and thereby, it can be applied to develop a novel drug. Further, this method can be used to analyze a characteristic such as change of the amount of carbohydrates in a glycoprotein derived from various cells as a drug candidate and thereby, is applicable for a quality control of proteins etc.