Abstract: The present invention relates to a tomato containing a high concentration of a precursor of vitamin D3, produced by using gene-editing technology, and a method for producing the same. Specifically, it has been confirmed that a tomato having the DWF5-1 gene deleted accumulate a high concentration of 7-dehydrocholesterol and that seeds can be produced therefrom. Thus, a tomato produced in such a way can contain a high concentration of a precursor of vitamin D3 and have a high commercial applicability.

Abstract: The present disclosure relates to a recombinant vector for plant expression system in which expression is increased by a radio frequency. According to the present disclosure, since the recombinant vector for plant expression of the present disclosure significantly increased the expression of the target protein in plants by treatment of an eco-friendly and biologically stable radio frequency, there is an effect of overcoming a low protein expression problem of a method using plant cells while having an advantageous effect capable of replacing a conventional production method using animal cells or microorganisms.

Abstract: The present disclosure provides compositions and methods for increasing mutation efficiency and homologous recombination rates of site-specific endonucleases. The compositions and methods comprise a chimeric polypeptide comprising a site-specific endonuclease or a domain thereof and a functional moiety. The current inventions relate to functional enhancement of the CRISPR-Cas enzymes. Disclosed herein include possible variants and their intended improvements.

Abstract: The present disclosure provides compositions and methods for increasing mutation efficiency and homologous recombination rates of site-specific endonucleases. The compositions and methods comprise a chimeric polypeptide comprising a site-specific endonuclease or a domain thereof and a functional moiety. The current inventions relate to functional enhancement of the CRISPR-Cas enzymes. Disclosed herein include possible variants and their intended improvements.

Abstract: The present disclosure provides compositions and methods for increasing mutation efficiency and homologous recombination rates of site-specific endonucleases. The compositions and methods comprise a chimeric polypeptide comprising a site-specific endonuclease or a domain thereof and a functional moiety. The current inventions relate to functional enhancement of the CRISPR-Cas enzymes. Disclosed herein include possible variants and their intended improvements.

Abstract: Methods for optimizing hotel revenues for a predetermined sale margin, optimizing room nights for a predetermined amount of margin, optimizing margin amounts for a predetermined amount of room nights, and reports based on the same are disclosed. Demand data is collected from conditional purchase offers submitted to the buyer-driven commerce system by potential buyers. Those conditional purchase offers which preferably are rejected are analyzed in order to determine parameters, which may result in increased revenues for the system operator of the buyer-driven commerce system or for participants who sell room nights through the system. Collected demand data may include an offer price, during which the buyer would like to purchase the room nights, or a promotional bump amount. Reports of expected changes in demand based upon the simulated changes to the business parameters are generated and presented to hotel operators, who may change acceptance parameters based on the reports.