Abstract: The present disclosure provides a sensor assembly for measuring a property of a sample. The sensing assembly comprises first and second dielectric layers. The first dielectric layer provides a well or aperture which is associated with an electrode. The second dielectric layer is provided on the first dielectric layer and provides an aperture fluidly connected to the well or aperture in the first dielectric layer.

Abstract: A biosensor comprising an electrode and inverted molecular pendulums (iMPs) is described. Each IMP includes a linker bound to the electrode, and an analyte receptor and a redox reporter both bound to the linker. The redox reporter is reactive at positive potential when the linker presents a net negative charge and reactive at negative potential when the linker presents a net positive charge. Upon application of an electric field, the biosensor is characterized by an iMPs unbound state, where no analyte is bound to the receptor, at which the iMPs are displaced towards the electrode and electron transfer from the iMPs towards the electrode occurs at an unbound electron transfer rate, and an iMPs bound state, where the analyte is bound to the receptor, at which the iMPs are displaced towards the electrode and electron transfer from the iMPs towards the electrode occurs at a bound electron transfer rate.