Patents by Inventor Suresh Vunnum
Suresh Vunnum has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 9144755Abstract: This invention relates to methods of using weak partitioning chromatography for the purification of a product from a load fluid containing one or more impurities. Further, the invention relates to methods of weak partitioning chromatography defined by operating conditions which cause a medium to bind least 1 mg of product per mL of medium, or alternatively, defined by a partition coefficient of at least 0.1.Type: GrantFiled: November 28, 2011Date of Patent: September 29, 2015Assignee: Wyeth LLCInventors: Paul R. Brown, Jonathan Lee Coffman, Ranganathan Godavarti, Timothy Iskra, Brian D. Kelley, Suresh Vunnum, Shujun Sun, Tianning Yu, James Edward Booth, Mary Beth Switzer
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Publication number: 20120149878Abstract: Methods of reducing high molecular weight species (HMW) formation in a sample containing a protein purified using ion exchange (IEX) chromatography are disclosed, as are a number of related methods, e.g., methods of reducing on-column denaturation of a protein in a protein sample purified using an ion exchange (IEX) column or resin. The methods share characteristics of including arginine, glycine and/or histidine in the buffers used during the ion exchange (IEX) chromatography.Type: ApplicationFiled: November 23, 2011Publication date: June 14, 2012Inventors: Ronald GILLESPIE, Suresh Vunnum, Thao Nguyen, Sean Macneil
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Publication number: 20120138537Abstract: This invention relates to methods of using weak partitioning chromatography for the purification of a product from a load fluid containing one or more impurities. Further, the invention relates to methods of weak partitioning chromatography defined by operating conditions which cause a medium to bind least 1 mg of product per mL of medium, or alternatively, defined by a partition coefficient of at least 0.1.Type: ApplicationFiled: November 28, 2011Publication date: June 7, 2012Applicant: Wyeth LLCInventors: Brian D. Kelley, James E. Booth, Paul Brown, Jon Coffman, Ranganathan Godavarti, Tim Iskra, Shujun Sun, Mary B. Switzer, Suresh Vunnum, Tianning Yu
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Patent number: 8067182Abstract: This invention relates to methods of using weak partitioning chromatography for the purification of a product from a load fluid containing one or more impurities. Further, the invention relates to methods of weak partitioning chromatography defined by operating conditions which cause a medium to bind least 1 mg of product per mL of medium, or alternatively, defined by a partition coefficient of at least 0.1.Type: GrantFiled: March 10, 2006Date of Patent: November 29, 2011Assignee: Wyeth LLCInventors: Brian D. Kelley, James E. Booth, Paul Brown, Jon Coffman, Ranganathan Godavarti, Tim Iskra, Shujun Sun, Mary B. Switzer, Suresh Vunnum, Tianning Yu
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Patent number: 7820796Abstract: Methods are provided for purification of Factor VIII polypeptides by affinity chromatography and ion exchange chromatography, in which the eluate from the affinity column is diluted with a solution comprising higher salt concentration, or lower non-polar agent concentration than that of the elution solution, prior to passing the diluted solution through the ion exchange column. The affinity matrix may comprise a monoclonal antibody or a peptide ligand. The methods result in improved purification without significant yield loss.Type: GrantFiled: January 26, 2005Date of Patent: October 26, 2010Assignee: Genetics Institute, LLC.Inventors: James Booth, Suresh Vunnum, Brian D. Kelley
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Publication number: 20070060741Abstract: This invention relates to methods of using weak partitioning chromatography for the purification of a product from a load fluid containing one or more impurities. Further, the invention relates to methods of weak partitioning chromatography defined by operating conditions which cause a medium to bind least 1 mg of product per mL of medium, or alternatively, defined by a partition coefficient of at least 0.1.Type: ApplicationFiled: March 10, 2006Publication date: March 15, 2007Applicant: WyethInventors: Brian Kelley, James Booth, Paul Brown, Jon Coffman, Ranganathan Godavarti, Tim Iskra, Shujun Sun, Mary Switzer, Suresh Vunnum, Tianning Yu
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Publication number: 20070054390Abstract: This invention relates to methods of using weak partitioning chromatography for the purification of a product from a load fluid. Further, the invention relates to methods of weak partitioning chromatography defined by operating conditions which cause a medium to bind least 1 mg of product per mL of medium, or alternatively, defined by a partition coefficient of at least 0.1.Type: ApplicationFiled: August 28, 2006Publication date: March 8, 2007Applicant: WyethInventors: Brian Kelley, James Booth, Paul Brown, Jon Coffman, Ranganathan Godavarti, Tim Iskra, Shujun Sun, Mary Switzer, Suresh Vunnum, Tianning Yu
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Publication number: 20050165221Abstract: Methods are provided for purification of Factor VIII polypeptides by affinity chromatography and ion exchange chromatography, in which the eluate from the affinity column is diluted with a solution comprising higher salt concentration, or lower non-polar agent concentration than that of the elution solution, prior to passing the diluted solution through the ion exchange column. The affinity matrix may comprise a monoclonal antibody or a peptide ligand. The methods result in improved purification without significant yield loss.Type: ApplicationFiled: January 26, 2005Publication date: July 28, 2005Applicant: WyethInventors: James Booth, Suresh Vunnum, Brian Kelley
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Publication number: 20040087776Abstract: Methods are provided for purification of Factor VIII polypeptides by immunoaffinity chromatography and ion exchange chromatography, in which the eluate from the immunoaffinity column is diluted with a solution comprising higher salt concentration, or lower non-polar agent concentration than that of the elution solution, prior to passing the diluted solution through the ion exchange column. The methods result in improved purification without significant yield loss.Type: ApplicationFiled: December 3, 2003Publication date: May 6, 2004Applicant: Genetics Institute, LLCInventors: Brian D. Kelley, Suresh Vunnum, Johanna Dalborg, Anna Petersson
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Patent number: 6683159Abstract: Methods are provided for purification of Factor VIII polypeptides by immunoaffinity chromatography and ion exchange chromatography, in which the eluate from the immunoaffinity column is diluted with a solution comprising higher salt concentration, or lower non-polar agent concentration than that of the elution solution, prior to passing the diluted solution through the ion exchange column. The methods result in improved purification without significant yield loss.Type: GrantFiled: December 29, 2000Date of Patent: January 27, 2004Assignee: Genetics Institute, LLCInventors: Brian D. Kelley, Suresh Vunnum, Johanna Dalborg, Anna Petersson
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Publication number: 20020115832Abstract: Methods are provided for purification of Factor VIII polypeptides by immunoaffinity chromatography and ion exchange chromatography, in which the eluate from the immunoaffinity column is diluted with a solution comprising higher salt concentration, or lower non-polar agent concentration than that of the elution solution, prior to passing the diluted solution through the ion exchange column. The methods result in improved purification without significant yield loss.Type: ApplicationFiled: December 29, 2000Publication date: August 22, 2002Inventors: Brian D. Kelley, Suresh Vunnum, Johanna Dalborg, Anna Petersson
