Abstract: Methods for purifying adenoviruses from a contaminated sample using a hydroxyapatite medium are provided. Sodium chloride concentrations present in buffers used throughout the method are at least 150 mM to prevent the adenovirus from irreversibly binding to the hydroxyapatite. Levels of contaminants and empty adenovirus capsids from samples purified by conventional purification techniques are further reduced to provide a highly pure adenovirus preparation.

Abstract: A computer implemented online music distribution system provides for the secure delivery of audio data and related media, including text and images, over a public communications network. The online music distribution system provides security through multiple layers of encryption, and the cryptographic binding of purchased audio data to each specific purchaser. The online music distribution system also provides for previewing of audio data prior to purchase. In one embodiment, the online music distribution system is a client-server system including a content manager, a delivery server, and an HTTP server, communicating with a client system including a Web browser and a media player. The content manager provides for management of media and audio content, and processing of purchase requests. The delivery server provides delivery of the purchased media data. The Web browser and HTTP server provide a communications interface over the public network between the content manager and media players.

Abstract: Content such as computer software, data representing audiovisual works, and electronic documents can converted from a machine-bound state to user-bound state without modification to the content data itself. Instead, keys used to access the content are converted from the machine-bound state to the user-bound state. In particular, the keys are kept in a passport data structure which can represent either a machine-binding or a user-binding. A machine-bound passport can be upgraded to a user-bound passport without modifying the bound content. The private key of the machine-bound passport, in cleartext form, is included in the user-bound passport and encrypted using a user-supplied password to bind the private key to the user. In addition, private user information is collected and verified and included in the user-bound passport.

Abstract: Content such as computer software, data representing audiovisual works, and electronic documents can converted from a machine-bound state to user-bound state without modification to the content data itself. Instead, keys used to access the content are converted from the machine-bound state to the user-bound state. In particular, the keys are kept in a passport data structure which can represent either a machine-binding or a user-binding. A machine-bound passport can be upgraded to a user-bound passport without modifying the bound content. The private key of the machine-bound passport, in cleartext form, is included in the user-bound passport and encrypted using a user-supplied password to bind the private key to the user. In addition, private user information is collected and verified and included in the user-bound passport.

Abstract: A computer implemented online music distribution system provides for the secure delivery of audio data and related media, including text and images, over a public communications network. The online music distribution system provides security through multiple layers of encryption, and the cryptographic binding of purchased audio data to each specific purchaser. The online music distribution system also provides for previewing of audio data prior to purchase. In one embodiment, the online music distribution system is a client-server system including a content manager, a delivery server, and an HTTP server, communicating with a client system including a Web browser and a media player. The content manager provides for management of media and audio content, and processing of purchase requests. The delivery server provides delivery of the purchased media data. The Web browser and HTTP server provide a communications interface over the public network between the content manager and media players.

Abstract: The present invention provides methods for isolating adjunct isoforms of proteins and converting them to the desired protein. In preferred embodiments, the present invention contemplates the use of acid solutions or zinc solutions to cleave a chemical group from a desired protein. In further preferred embodiments, the present invention contemplates the oxidation of reduced sulfhydryl groups with cleavage of chemical groups to form a functional desired protein.

Abstract: Disclosed is a method for purifying Interleukin-10 (IL-10). The method is comprised of subjecting an IL-10 containing solution to cation exchange chromatography, anion exchange chromatography, hydroxyapatite chromatography, and gel filtration chromatography. The present invention is also comprised of a process for separating different IL-10 dimers present in a protein fraction from each other by subjecting the protein fraction to hydroxyapatite chromatography. The present invention is also comprised of a process for separating variants of a protein differing in an N-terminal amino acid sequence present in a protein fraction from each other by subjecting the protein fraction to hydroxyapatite chromatography.

Abstract: A method for purifying Interleukin-10 (Il-10) from a eukaryotic cell culture medium. The method is comprised of subjecting IL-10 containing cell culture medium to cation exchange chromatography, anion exchange chromatography, hydroxyapatite chromatography, and gel filtration chromatography. The present invention is also comprised of a process for separating different IL-10 dimers present in a protein fraction from each other by subjecting the protein fraction to hydroxyapatite chromatography. The present invention is also comprised of a process for separating variants of a protein differing in an N-terminal amino acid sequence present in a protein fraction from each other by subjecting the protein fraction to hydroxyapatite chromatography.