Abstract: According to some embodiments a system is provided. The system comprises a remote monitoring computing system that is configured to receive an alarm signal from a premises monitoring system that is configured to monitor a premises where the alarm signal is associated with an alarm event at the premises, in response to the alarm signal, obtain, from a content storage computing system, metadata corresponding to video provided by a camera at the premises, in response to the metadata, store the video in a data store of the remote monitoring computing system, and enforce an access control policy on the video in the data store where the access control policy restricts access to the video in the data store based on time and a plurality of roles of a plurality of users of the remote monitoring computing system.

Abstract: An apparatus to supply print fluid (9) to a print fluid delivery system (28) of a printer, the apparatus comprising a tank (4) to store print fluid (9); a print fluid supply conduit (12) coupled to the tank (4) to supply print fluid (9) to the print fluid delivery system (28); and a vent conduit (10) extending from the tank (4); the tank (4) movable from a first position to supply print fluid (9) and in which first position the print fluid supply conduit (12) and vent conduit (10) are open to a second position to permit filling of the tank (4) and in which movement to the second position closes the print fluid supply conduit (12) and vent conduit (10).

Abstract: An apparatus to supply print fluid (19) to a print fluid delivery system (28) of a printer, the apparatus comprising a tank (4) to store print fluid (19); a print fluid supply conduit (12) coupled to the tank (4) to supply print fluid (19) to the print fluid delivery system (28); and a vent conduit (10) extending from the tank (4); the tank (4) movable from a first position to supply print fluid (19) and in which first position the print fluid supply conduit (12) and vent conduit (10) are open to a second position to permit filling of the tank (4) and in which movement to the second position closes the print fluid supply conduit (12) and vent conduit (10).

Abstract: The present invention is directed to a method of producing organisms tolerant to alcohol, that includes selecting a microorganism needing tolerance to alcohol and modifying the selected microorganism under conditions effective to overproduce inositol by the microorganism compared to when the microorganism is not modified, with the modified microorganism being tolerant to alcohol. The present invention is also directed to a method of producing alcohol that includes providing a microorganism tolerant to alcohol which is modified to overproduce inositol by the microorganism compared to when the microorganism is not modified. A fermentable feedstock is treated with the modified microorganism under conditions effective to produce the alcohol. The modified microorganism is also able to produce and tolerate alcohol in high osmolarity feedstocks.

Abstract: Methods and materials are provided for stably introducing any gene into a specific locus in the genome of a microorganism such as yeast without the addition of any drug resistance genes. Specifically provided herein are new genetically engineered inositol-overproducing Saccharomyces cerevisiae strains obtained by using a novel set of yeast integration plasmids that allow the safe, stable, and controlled introduction of homologous as well as heterologous genes into the host genome. In particular, specific loci of the S. cerevisiae yeast genome can be targeted with single or multiple copies of a specific gene that is desired to be expressed or a given set of specific genes that the host can use without the addition of any drug resistance genes. The principles of this new methodology can also be used for the construction of other recombinant yeast and bacterial strains as well as higher eukaryotic cells.

Abstract: Disclosed are compositions and methods for increasing the nutritional value of plants and plant parts. In illustrative embodiments PEAMT and ?PEAMT polynucleotide and polypeptide compositions are disclosed as well as their use in modulating the levels of lipid compounds, and particularly, regulating the level of phosphatidylcholine, and its precursors in plants and seeds derived therefrom. Also disclosed are methods for modulating the level of glycine betaine and choline-O-sulfate in cells, and increasing the tolerance of transformed plants to osmotic and cryogenic stress.

Abstract: Methods and materials are provided for stably introducing any gene into a specific locus in the genome of a microorganism such as yeast without the addition of any drug resistance genes. Specifically provided herein are new genetically engineered inositol-overproducing Saccharomyces cerevisiae strains obtained by using a novel set of yeast integration plasmids that allow the safe, stable, and controlled introduction of homologous as well as heterologous genes into the host genome. In particular, specific loci of the S. cerevisiae yeast genome can be targeted with single or multiple copies of a specific gene that is desired to be expressed or a given set of specific genes that the host can use without the addition of any drug resistance genes. The principles of this new methodology can also be used for the construction of other recombinant yeast and bacterial strains as well as higher eukaryotic cells.

Abstract: Methods and materials are provided for stably introducing any gene into a specific locus in the genome of a microorganism such as yeast without the addition of any drug resistance genes. Specifically provided herein are new genetically engineered inositol-overproducing Saccharomyces cerevisiae strains obtained by using a novel set of yeast integration plasmids that allow the safe, stable, and controlled introduction of homologous as well as heterologous genes into the host genome. In particular, specific loci of the S. cerevisiae yeast genome can be targeted with single or multiple copies of a specific gene that is desired to be expressed or a given set of specific genes that the host can use without the addition of any drug resistance genes. The principles of this new methodology can also be used for the construction of other recombinant yeast and bacterial strains as well as higher eukaryotic cells.

Abstract: Methods are provided for increased and controlled production of choline and inositol, choline and inositol metabolites, and choline- and inositol-containing phospholipids in yeast, comprising the step of increasing phosphatidylcholine (PC) turnover in yeast. Methods are also provided for detecting phospholipase D-mediated turnover of phosphatidylcholine in vivo in yeast, comprising an assay for choline excretion.

Abstract: A yeast cell, preferably Saccharomyces cerevisiae, which contains a functional stable recombinant DNA sequence that does not allow for the encoding of a negative regulator of phospholipid biosynthesis therein. In a preferred embodiment, the recombinant DNA sequence is an opil gene deletion which results in the deregulation of inositol or inositol-containing metabolites such as inositol-1-phosphate synthase. Moreover, there is a method for obtaining inositol, inositol-containing metabolites or phospholipids such as myo-inositol or inositol-1-phosphate. The method comprises the steps of genetically engineering a stable yeast cell, preferably Saccharomyces cerevisiae, to continually produce inositol, inositol-containing metabolites or phospholipids. Additionally, there is the step of then generating the inositol, inositol-containing metabolites or phospholipids.

Abstract: A yeast cell, preferably Saccharomyces cerevisiae, which contains a functional stable recombinant DNA sequence that does not allow for the encoding of a negative regulator of phospholipid biosynthesis therein and which has multiple copies of an INO1 gene. In a preferred embodiment, the recombinant DNA sequence is an OPI1 gene deletion which results in the deregulation of inositol or inositol-containing metabolites such as inositol-1-phosphate synthase. Moreover, there is a method for obtaining inositol, inositol-containing metabolites or phospholipids such as myo-inositol or inositol-1-phosphate. The method comprises the steps of genetically engineering a stable yeast cell, preferably Saccharomyces cerevisiae, to continually produce inositol, inositol-containing metabolites or phospholipids. Additionally, there is the step of then generating the inositol, inositol-containing metabolites or phospholipids.