Abstract: Provided herein are capillaries for use in an electrophoretic separations. The capillaries can comprise an elongated tubular wall defining a path for fluid flow from an inlet to a tapered outlet; and a decoupler junction positioned within the elongated tubular wall upstream of the tapered outlet. The decoupler junction can comprise a plurality of microchannels penetrating through the elongated tubular wall and an ion-permeable polymer coating the plurality of microchannels. Also provided are sheathless interfaces for coupling capillary electrophoresis (CE) with mass spectrometry that employ these capillaries.

Abstract: Provided herein are capillaries for use in an electrophoretic separations. The capillaries can comprise an elongated tubular wall defining a path for fluid flow from an inlet to a tapered outlet; and a decoupler junction positioned within the elongated tubular wall upstream of the tapered outlet. The decoupler junction can comprise a plurality of microchannels penetrating through the elongated tubular wall and an ion-permeable polymer coating the plurality of microchannels. Also provided are sheathless interfaces for coupling capillary electrophoresis (CE) with mass spectrometry that employ these capillaries.

Abstract: An interface is provided for use with a multiple sample acquisition device and a capillary electrophoresis separation device. The interface includes a body member having a base and an upstanding wall defining a generally hollow interior well. The upstanding wall includes an inlet passageway and an outlet passageway. An inlet guide member has an axially extending guide passageway disposed coaxially with the inlet passageway. An outlet guide member has an axially extending guide passageway disposed coaxially with the outlet passageway. An inlet capillary tube has an outer diameter sized for being received by the axially extending passageway of the inlet guide member, and a fluid passageway through which analyte can flow. The inlet capillary tube also includes a first end disposed exteriorly of the well and a second end disposed within the well.

Abstract: Improved capillary electrophoresis (CE) apparatus (10) is provided which includes an intermediate grounding joint (34) serving to isolate delicate electrochemical detectors (32, 54, 58) from the high voltage CE power supply (20). The joint (34) includes a fracture (36) close to the detection end (22) of the apparatus (10) which is covered by a Nafion sleeve (38). The joint (34) is immersed in an ionic buffer reservoir (46) which includes a grounded cathode (48). In fabrication procedures, a capillary tube (12) is scored in proximity to one end (22) thereof, and the Nafion sleeve (38) is slid over the score line. The sleeve (38) is then sealed with epoxy (40, 42) at the ends thereof.