Abstract: The present disclosure relates to compositions, apparatus and methods for generating one or more scaffolds, including: mixing a hydrogel material and/or an extracellular matrix (ECM) protein in an aqueous solvent to generate an aqueous process solution; and cryoelectrospinning the aqueous process solution onto a plurality of conductive probes extending from a conductive surface of a collector plate disposed within a process chamber under conditions sufficient to generate one or more scaffolds configured to mimic a preselected soft tissue decellularized extracellular matrix. Scaffold compositions are also provided having preselected or tuned characteristics. The scaffolds provide a promising stromal cell delivery vehicle for the remediation of fibrosis.

Abstract: The present disclosure relates to compositions, apparatus and methods for generating one or more scaffolds, including: mixing a hydrogel material and/or an extracellular matrix (ECM) protein in an aqueous solvent to generate an aqueous process solution; and cryoelectrospinning the aqueous process solution onto a plurality of conductive probes extending from a conductive surface of a collector plate disposed within a process chamber under conditions sufficient to generate one or more scaffolds configured to mimic a preselected soft tissue decellularized extracellular matrix. Scaffold compositions are also provided having preselected or tuned characteristics.

Abstract: Provided is a system for modeling the conventional outflow tract, including: a porous scaffold with trabecular meshwork cells attached to one surface and microvascular endothelial cells co-cultured on an opposite surface transforming the microvascular endothelial cells into Schlemm's canal cell-like cells. Also provided is a method for using the system for screening by contacting the cells with a known or suspected medicament and measuring its effects on the system such as flow of a perfusate. Also provided is a method of making the system by fabricating the porous substrate as a micropatterned scaffold.

Abstract: The present invention relates to methods of manufacture and utility of an artificial trabecular meshwork [TM] that utilizes micro- and nanofabricated materials bioengineered to mimic the structure and function of native outflow system of the eye.