Abstract: The present invention is directed to new compositions that are described for the simultaneous, controlled dose delivery of a variety of biomolecules into phagocytic cells.

Abstract: An improved vaccine for immunization of waterfowl such as ducks and geese comprises an inactivated strain of a Mycoplasma infecting waterfowl, such as Mycoplasma sp. strain 1220; the vaccine can include an excipient and an adjuvant. Methods for immunization of waterfowl with the vaccine are also described.

Abstract: An improved vaccine for immunization of waterfowl such as ducks and geese comprises an inactivated strain of a mycoplasma infecting waterfowl, such as Mycoplasma sp. strain 1220; the vaccine can include an excipient and an adjuvant. Methods for immunization of waterfowl with the vaccine are also described.

Abstract: This invention provides a microparticle carrier system comprising of one or more proteins, peptides, nucleic acids, carbohydrates, lipids or other bioactive substances with or without targeting molecules attached. In addition, the invention also provides immune modulatory compositions and methods of eliciting protective immune responses both in uninfected and infected hosts as well as the induction of immune tolerance.

Abstract: An improved vaccine for immunization of waterfowl such as ducks and geese comprises an inactivated strain of a mycoplasma infecting waterfowl, such as Mycoplasma sp. strain 1220; the vaccine can include an excipient and an adjuvant. Methods for immunization of waterfowl with the vaccine are also described.

Abstract: This invention provides a microparticle carrier system comprising of one or more proteins, peptides, nucleic acids, carbohydrates, lipids or other bioactive substances with or without targeting molecules attached. In addition, the invention also provides immune modulatory compositions and methods of eliciting protective immune responses both in uninfected and infected hosts as well as the induction of immune tolerance.

Abstract: This invention provides a microparticle carrier system comprising of one or more proteins, peptides, nucleic acids, carbohydrates, lipids or other bioactive substances with or without targeting molecules attached. In addition, the invention also provides immune modulatory compositions and methods of eliciting protective immune responses both in uninfected and infected hosts as well as the induction of immune tolerance.

Abstract: The present invention is directed to new compositions that are described for the simultaneous, controlled dose delivery of a variety of biomolecules into phagocytic cells.

Abstract: This invention provides a microparticle carrier system comprising of one or more proteins, peptides, nucleic acids, carbohydrates, lipids or other bioactive substances with or without targeting molecules attached. In addition, the invention also provides immune modulatory compositions and methods of eliciting protective immune responses both in uninfected and infected hosts as well as the induction of immune tolerance.

Abstract: In general, a method for removal of pyrogens from plasma or blood according to the present invention comprises: (1) passing the biological fluid through a resin comprising two components: (a) a first component comprising a polymeric support with a multiplicity of ligands comprising a C10-C24 alkylamino group, the ligands specifically binding endotoxin; and (b) a second component comprising a halogenated quinolone moiety immobilized on an amino-group-containing solid support by the formation of an amide linkage, the first component and the second component being present in the resin at a ratio of from about 20:80 (v/v) to about 80:20 (v/v); and (2) collecting the biological fluid from which pyrogens have been removed. Preferably, the alkylamino group is a stearylamino group, and the ligands are cross-linked and quaternized by reaction with a dibromoalkanol. Preferably, the polymeric support of the first component is agarose in particulate form.

Abstract: In general, a method for removal of pyrogens from plasma or blood according to the present invention comprises: (1) passing the biological fluid through a resin comprising two components: (a) a first component comprising a polymeric support with a multiplicity of ligands comprising a C10-C24 alkylamino group, the ligands specifically binding endotoxin; and (b) a second component comprising a halogenated quinolone moiety immobilized on an amino-group-containing solid support by the formation of an amide linkage, the first component and the second component being present in the resin at a ratio of from about 20:80 (v/v) to about 80:20 (v/v); and (2) collecting the biological fluid from which pyrogens have been removed. Preferably, the alkylamino group is a stearylamino group, and the ligands are cross-linked and quaternized by reaction with a dibromoalkanol. Preferably, the polymeric support of the first component is agarose in particulate form.

Abstract: The present invention provides a decorative cosmetic composition that is visually distinct comprising a translucent or transparent particle and a cosmetic base. In addition, the present invention provides a decorative cosmetic composition that has both a functional effect, such as for cleansing or massage, and a distinct visual effect.

Abstract: A new method is described to produce large volumes of low cost water for injection directly from potable water in order to meet the needs of hemodialysis and other biological applications for ultrapure water. The source water is processed by a combination of membrane and chromatographic methods including reverse osmosis, ion exchange deionization, endotoxin-specific adsorption steps and polishing filtration to reduce contaminant levels below those specified by the US Pharmacopoiea. Endotoxin removal from dialysate was demonstrated. The endotoxin removing adsorbent also adsorbs LAL-negative pyrogens from the test samples.

Abstract: A method and device is described for direct removal of heparin from whole blood during extracorporeal therapy. In clinical situations where the blood is heparinized to prevent clotting in the extracorporeal circuit containing e.g., a hemodialysis cartridge or a heart-lung machine, it would be desirable to eliminate the systemic heparinization of the patients. The extracorporeal circuit contains a device having antithrombin III immobilized and inserted between the outlet port of the primary extracorporeal device, such as the hemodialysis cartridge or heart-lung machine and the patient. When the heparinized blood comes in contact with the immobilized antithrombin III, heparin is removed and the reperfused blood is substantially free of heparin. The advantage of the system lies in the fact that heparin is removed from the blood rather than neutralized. This helps overcome the adverse reactions and side effects associated with the use of heparin anticoagulation.

Abstract: The recovery of immunoselected cells is improved by using a specific affinity elution system utilizing members of an immobilized ligand/antiligand pair, with soluble ligand used for elution. In one embodiment, Immobilized heparin adsorbent is coated with biotinylated antithrombin III which is then crosslinked with avidin, thereby greatly increasing the binding affinity of antithrombin III for heparin. The resulting adsorbent effectively captures biotin-labeled target cells and allow facile elution of immunoselected cells with soluble heparin. The cell separation system is capable of both positive and negative selection.

Abstract: An integrated cell culture-protein purification system has been developed for the continuous, automated production of pure cell culture protein products. The instrument comprises a bioreactor subunit having a hollow fiber bioreactor to culture and maintain cells which secrete the desired product into the cell culture medium. The culture medium is circulated through a purification cartridge which adsorbs the desired product. The system is capable of continuously removing the product from the culture medium by immunoaffinity adsorption and pure product is then recovered. The product can also be recovered automatically in a discontinuous operation from the spent culture medium by either affinity, ion exchange, or hydrophobic purification techniques. The integrated system allows continuous production of high-quality, pure proteins from cell culture.

Abstract: A method as well as large bead chromatography particles are described for the direct capture of a desired product from unclarified process liquor on standard, low pressure, packed bed chromatography columns. The beads are sufficiently large so that the particulate contaminants can flow in the interparticle lumen and exit the column without obstructing the flow. The desired product is simultaneously captured by the functional groups of the beads. The beads have high dynamic capacities and allow rapid processing of large volumes of process materials.