Abstract: A bike-holding system for transporting one or more two-wheeled vehicles in a bed of a transportive vehicle includes a first holder comprising a plate interrupted by one or more elongated slots, each slot being adapted to receive a first wheel of a two-wheeled vehicle, which first wheel may be either a front or rear wheel. The plate is inclined at an angle to the plane of the bed. The system may include a second holder comprising a second plate interrupted by one or more elongated slots or pairs of rails, each of the elongated slots or rail pairs being adapted to receive a second wheel of the two-wheeled vehicle. A two-wheeled vehicle thus installed in a holder or holders of the invention is restrained from lateral (tipping) motion or vertical motion with respect to the bed by one or more hold-downs attached to the two-wheeled vehicle and the transporting vehicle.